CD305 (LAIR1) Monoclonal Antibody (NKTA255), PE, eBioscience™, Invitrogen™

Description

• Description: This NKTA255 monoclonal antibody reacts with human CD305, which is also known as leukocyte-associated Ig-like receptor (LAIR) 1
• CD305 is a type I transmembrane glycoprotein that possesses an extracellular Ig-like domain and two intracellular ITIMs
• CD305 is expressed on nearly all immune cells, including T, NK, and B cells, as well as monocytes, dendritic cells, eosinophils, basophils, and mast cells
• In humans, this molecule is also expressed on CD34+ hematopoietic progenitor cells and thymocytes
• Several isoforms of CD305 exist due to alternative splicing, and their expression may differ between cell types
• As an inhibitory receptor, CD305 blocks cytokine-mediated signaling, cell proliferation, and apoptosis
• Studies have also identified collagens as the ligands for CD305
• The NKTA255 antibody has been reported to inhibit NK cytolytic activity
• Applications Reported: This NKTA255 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This NKTA255 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood cells
• This can be used at 5 μL (0.06 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
• LAIR1 (CD305) functions as an inhibitory receptor that plays a constitutive negative regulatory role on cytolytic function of natural killer (NK) cells, B-cells and T-cells
• Activation by Tyr phosphorylation results in recruitment and activation of the phosphatases PTPN6 and PTPN11
• It also reduces the increase of intracellular calcium evoked by B-cell receptor ligation
• May also play its inhibitory role independently of SH2-containing phosphatases
• Modulates cytokine production in CD4+ T-cells, down-regulating IL2 and IFNG production while inducing secretion of transforming growth factor beta
• Down-regulates also IgG and IgE production in B-cells as well as IL8, IL10 and TNF secretion
• Inhibits proliferation and induces apoptosis in myeloid leukemia cell lines as well as prevents nuclear translocation of NF-kappa-B p65 subunit/RELA and phosphorylation of I-kappa-B alpha/CHUK in these cells
• Inhibits the differentiation of peripheral blood precursors towards dendritic cells.