CD85j (ILT2) Monoclonal Antibody (HP-F1), PE-eFluor™ 610, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody HP-F1 recognizes CD85j, also known as ILT2, LILRB1, and LIR1
• CD85j is a member of the ILT (immunoglobulin-like transcript)/LIR (leukocyte Ig-like receptor)/MIR (monocyte Ig-like receptor) family
• CD85j is a single transmembrane glycoprotein with a long cytoplasmic domain containing 4 ITIMs which signal through interactions with SHP-1
• Expression is found on myeloid cells (monocytes and dendritic cells) and some lymphoid cells including, subsets of NK, T and B cells
• Expression has been correlated with leukemias such as ALL and CLL
• Expression on CD8+ cells correlates with effector cell function and plays an important role in viral infections, including HIV, Ebstein Barr and CMV
• The ligands for CD85j are MHC Class I molecules such as HLA-G, A, F, B27, E and F
• Applications Reported: This HP-F1 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This HP-F1 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
• This may be used at 5 μL (0.125 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• PE-eFluor 610 can be excited with laser lines from 488-561 nm and emits at 607 nm
• We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red)
• This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4
• The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs)
• The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response
• It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity
• Multiple transcript variants encoding different isoforms have been found for this gene.