CD94 Monoclonal Antibody (HP-3D9), APC, eBioscience™, Invitrogen™

Description

• Description: The HP-3D9 monoclonal antibody reacts with human CD94, a 70-kDa type II transmembrane glycoprotein
• CD94 belongs to the C-type lectin superfamily and is expressed by NK cells, NKT cells, as well as subsets of CD8+ T cells and gamma delta T cells
• This molecule is present as a heterodimer with members of the NKG2 family of receptors on the cell surface
• CD94 dimerization with NKG2A results in an inhibitory receptor that suppresses NK cell function
• In contrast, CD94 association with NKG2C and NKG2E leads to formation of an activating receptor complex that binds DAP12, an adapter protein that contains an ITAM motif
• The CD94/NKG2 receptor complex binds the non-classical MHC Class Ib molecule HLA-E and plays important roles in adhesion and NK cell activation
• Crossblocking studies indicate that HP-3D9 and DX22 recognize the same epitope
• Applications Reported: This HP-3D9 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This HP-3D9 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells
• This can be used at 5 μL (0.125 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
• Filtration: 0.2 μm post-manufacturing filtered
• Natural killer (NK) cells, like CD94, are a distinct lineage of lymphocytes that mediate cytotoxic activity and secrete cytokines upon immune stimulation
• Several genes of the C-type lectin superfamily, including members of the NKG2 family, are expressed by NK cells and may be involved in the regulation of NK cell function
• KLRD1 (CD94) is an antigen preferentially expressed on NK cells and is classified as a type II membrane protein because it has an external C terminus
• Three transcript variants encoding two different isoforms have been found for this gene.