CD169 (Siglec-1) Monoclonal Antibody (7-239), APC, eBioscience™, Invitrogen™

Description

• Description: This 7-239 monoclonal antibody reacts with human CD169, also known as Siglec-1 and Sialoadhesin
• This type I transmembrane protein is a member of the sialic acid binding Ig-like lectin (Siglec) family
• Expressed on macrophages and dendritic cells in peripheral blood, lymph nodes, and spleen in humans, CD169 binds glycoproteins and glycolipids containing N-acetylneuraminyl alpha 2-3-galactose
• CD169 expression can also be induced on CD14+ monocytes by interferon-alpha and interferon-gamma to mediate HIV-1 infection
• Finally, CD169 has also been shown to be involved in antigen presentation to invariant NK T cells
• This monoclonal antibody has been reported to inhibit erythrocyte binding to CD169-expressing cells
• Applications Reported: This 7-239 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 7-239 antibody has been pre-titrated and tested by flow cytometric analysis of cultured human peripheral blood cells
• This can be used at 5 μL (0.06 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
• Filtration: 0.2 μm post-manufacturing filtered
• The siglecs are a family of membrane bound lectins (of the immunoglobulin superfamily) that bind sialic acid and mediate cell-cell interactions
• Family members include sialoadhesin, CD22 and CD33
• Sialoadhesin is a macrophage-specific cell adhesion molecule that is expressed on activated macrophages in chronic inflammation and in tumours.