CD170 (Siglec F) Monoclonal Antibody (1RNM44N), Super Bright™ 645, eBioscience™, Invitrogen™

Description

• Description: The 3D12 monoclonal antibody reacts with human CCR7, also known as EBI-1 and CD197
• CCR7 is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains and functions as a receptor for 6Ckine/SLC (secondary lymphoid-tissue chemokine), CCL19 and CCL21
• CCR7 has been shown to be internalized via clathrin-coated pits and the majority recycled back to the plasma membrane
• CCR7 is expressed on T cells and can be used to distinguish populations of naive from central and effector memory T cells
• CCR7 has been shown to play a role in migration of memory T cells to inflamed tissue
• Expression of CCR7 is also found on DC's
• During DC maturation CCR7 expression increases and is thought to be involved in a variety of functions: chemotaxis to the lymph node, cellular architecture, rate of endocytosis, survival and maturation
• Expression of CCR7 on the cell surface can be down regulated upon ligand binding
• Applications Reported: This 3D12 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 3D12 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
• This may be used at 5 μL (1 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• SIGLEC5 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells
• This protein binds equally to alpha2,3-linked and alpha2,6-linked sialic acid
• The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface
• This molecule is expressed by monocytic/myeloid lineage cells
• SIGLEC5 is found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus
• It is expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation.