CD170 (Siglec F) Monoclonal Antibody (1RNM44N), Super Bright™ 436, eBioscience™, Invitrogen™

Description

• Description: This 1RNM44N monoclonal antibody recognizes mouse CD170, also known as Siglec F
• Siglec F is a cell surface lectin belonging to the Ig superfamily that binds glycoconjugates containing sialic acids that are commonly found on various cell types
• The cytoplasmic domain of Siglec F contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) that initiates intracellular signaling upon ligand binding
• Siglec F is expressed mostly on eosinophils and alveolar macrophages and lower levels of this receptor have also been reported on immature myelomonocytic cells
• Mouse Siglec F is a functional ortholog of human Siglec 8, however, unlike human Siglec 8, mouse Siglec F is not expressed on mast cells
• This 1RNM44N antibody will recognize a formaldehyde-fixed epitope
• Applications Reported: This 1RNM44N antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 1RNM44N antibody has been tested by flow cytometric analysis of thioglycolate-elicited peritoneal macrophages
• This may be used at less than or equal to 0.125 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm
• SIGLEC5 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells
• This protein binds equally to alpha2,3-linked and alpha2,6-linked sialic acid
• The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface
• This molecule is expressed by monocytic/myeloid lineage cells
• SIGLEC5 is found at high levels in peripheral blood leukocytes, spleen, bone marrow and at lower levels in lymph node, lung, appendix, placenta, pancreas and thymus
• It is expressed by monocytes and neutrophils but absent from leukemic cell lines representing early stages of myelomonocytic differentiation.