c-Rel Monoclonal Antibody (1RELAH5), PE, eBioscience™, Invitrogen™

Description

• Description: The 1RELAH5 monoclonal antibody reacts with mouse c-Rel
• c-Rel is a 65 kDa hematopoietic lineage-restricted member of the NF-kB family of transcription factors
• In the thymus, c-Rel is upregulated in regulatory T cells (Treg) when compared to other CD4+ thymocytes
• c-Rel is believed to play a critical role in thymic Treg (nTreg) development, but is not necessary for TGF beta-mediated T reg (iTreg) differentiation
• C-Rel is also involved in proliferation and survival in mature T cells
• Applications Reported: This 1RELAH5 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
• Applications Tested: This 1RELAH5 antibody has been tested by intracellular staining and flow cytometric analysis of mouse thymocytes using the Foxp3 Fixation/Permeabilization Buffers (cat
• 00-5521) and protocol
• This can be used at less than or equal to 0.125 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser
• Filtration: 0.2 μm post-manufacturing filtered
• The REL gene encodes c-Rel, a transcription factor that is a member of the Rel/NFKB family, which also includes RELA., RELB (604758), NFKB1, and NFKB2
• These proteins are related through a highly conserved N-terminal region termed the 'Rel domain, ' which is responsible for DNA binding, dimerization, nuclear localization, and binding to the NFKB inhibitor (Belguise and Sonenshein, 2007).