NOTCH1 Monoclonal Antibody (22E5), PE, eBioscience™, Invitrogen™

Description

• Description: This 22E5 monoclonal antibody reacts with the extracellular domain of mouse Notch1, one of four members of the Notch family of receptors
• Notch receptors are 300-kDa single-pass transmembrane proteins
• While the extracellular domain contains numerous epidermal growth factor-like repeats for ligand binding, the intracellular domain is involved in cell signaling
• Upon binding its membrane-bound ligand (either Delta or Jagged), the Notch receptor undergoes proteolytic cleavage, first by ADAM-family metalloproteases and then by gamma-secretase
• The second cleavage event releases the Notch intracellular domain (NICD), which subsequently translocates into the nucleus, heterodimerizes with the DNA-binding protein RBP-J, recruits co-activator molecules, and ultimately activates transcription
• Notch 1 is expressed on thymocytes, bone marrow hematopoietic stem cells, T and NK cells
• Lower Notch1 expression levels can be found on B cells and monocytes
• Studies show that some subsets of T-cell acute lymphoblastic leukemia (T-ALL) arise due to Notch1 chromosomal translocation with TCRbeta, which results in the expression of constitutively active Notch1
• This cell surface receptor is involved in T cell lineage commitment, thymocyte development, and Th2 differentiation
• Applications Reported: This 22E5 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 22E5 antibody has been tested by flow cytometric analysis of mouse thymocytes
• The notch gene belongs to a family of epidermal growth factor (EGF) like homeotic genes, which encode transmembrane proteins with a variable number of cysteine-rich EGF-like repeats in the extracellular region
• Four notch genes have been described in mammals: Notch1, Notch2, Notch3 and Notch4(Int-3), which have been implicated in the differentiation of the nervous system and other structures
• The EGF-like proteins Delta and Serrate have been identified as ligands of Notch1
• Mature Notch proteins are heterodimeric receptors derived from the cleavage of Notch pre-proteins into an extracellular subunit (NEC) containing multiple EGF-like repeats and a transmembrane subunit including intracellular region (Ntm).