MERTK Monoclonal Antibody (DS5MMER), Super Bright™ 702, eBioscience™, Invitrogen™

Description

• Description: This JAV4 monoclonal antibody recognizes human V-Set and Immunoglobulin domain containing 4 (VSIG4), also known as Complement Receptor of the Immunoglobulin superfamily (CRIg) or Z39Ig
• There are two variants of human VSIG4 that result from alternative splicing
• Isoform 1 is longer and more prevalent than isoform 2, and this JAV4 antibody recognizes both
• VSIG4 is a type I transmembrane glycoprotein structurally related to the B7 family of immune regulatory proteins
• It contains one complete V-type Ig domain and one truncated C-type Ig domain
• VSIG4 is exclusively expressed on tissue resident and tumor infiltrating macrophages
• It has been shown to bind complement components C3b and iC3b
• This binding inhibits the alternative complement pathway and facilitates phagocytosis of complement-opsonized pathogens
• VSIG4 has also been reported to suppress T cell activation, proliferation and IL-2 production thereby playing a role in the maintece of peripheral T cell tolerance and suppression of established inflammation
• Expression of VSIG4 on tumor-infiltrating macrophages suggests its role in immune evasion
• Pro-inflammatory stimuli such as TNF and LPS have been reported to down-regulate the expression of VSIG4
• The JAV4 antibody will recognize VSIG4 on cells that have been formaldehyde-fixed and permeabilized
• This antibody does not cross-react with mouse or rat VSIG4
• Applications Reported: This JAV4 antibody has been reported for use in flow cytometric analysis
• MERTK (cMER) is a tyrosine kinase proto-oncogene and is involved in the retinal pigment epithelium (RPE) phagocytosis pathway, which is implicated in human retinal disease
• Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis)
• Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules
• Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1
• MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment
• Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis
• Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.