CD142 Monoclonal Antibody (HTF-1), APC, eBioscience™, Invitrogen™

Description

• Description: This HTF-1 monoclonal antibody reacts with human CD142, which is also known as Tissue Factor
• Expression of this type I transmembrane glycoprotein on endothelial cells, monocytes, macrophages, and platelets can be induced by inflammatory mediators (e.g., LPS, IL-1b, TNFa, PMA, or endotoxin)
• On the other hand, CD142 is expressed constitutively by some tumor cells (e.g., lung, pancreatic, breast, and colon) and non-immune tissues such as the vasculature, central nervous system, kidney, epithelia, and placenta
• Studies have also suggested that CD142 exists as a soluble form that circulates in blood
• CD142 initiates blood coagulation by associating with and activating the circulating factors VII and VIIa
• The HTF-1 antibody has been reported to exhibit blocking activity
• Applications Reported: This HTF-1 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This HTF-1 antibody has been pre-titrated and tested by flow cytometric analysis of LPS-stimulated normal human peripheral blood cells
• This can be used at 5 μL (0.06 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser
• Filtration: 0.2 μm post-manufacturing filtered
• This gene encodes coagulation factor III which is a cell surface glycoprotein
• This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII
• The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis
• Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces
• There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic
• This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described.