FOXP3 Monoclonal Antibody (FJK-16s), eFluor™ 615, eBioscience™, Invitrogen™

Description

• Description: The FJK-16s antibody reacts with mouse, rat, dog, porcine, bovine and cat Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined
• Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice
• Constitutive high expression of foxP3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells
• Immunoblotting with FJK-16s antibody has mapped the epitope to amino acids 75-125 of the mouse Foxp3 protein
• In the human, this region has been shown to be alternatively spliced at the mRNA level
• Both the alternatively-spliced and non-spliced isoforms are present in the CD4+CD25+ subset of lymphocytes
• Preliminary RT-PCR experiments have not revealed this alternatively-spliced isoform in mouse splenocytes, suggesting different gene regulation in the mouse and human
• Applications Reported: This FJK-16s antibody has been reported for use in immunohistochemical staining of frozen tissue (IHC-F), and immunocytochemistry (ICC)
• Applications Tested: This FJK-16s antibody has been tested by immunohistochemistry on frozen mouse spleen (IHC-F) at less than or equal to 10 μg/mL
• This product has not been validated for flow cytometric analysis
• FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis
• FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells
• FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells
• Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice
• In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome
• Transcript variants of FOXP3 encoding different isoforms have been identified
• In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.