ErbB3 (HER-3) Monoclonal Antibody (SGP1), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Description

• Description: This SGP1 monoclonal antibody reacts with human c-ErbB3, which is also known as HER3
• This epidermal growth factor (EGF) receptor family member is expressed on normal human tissues, particularly on neurons and cells of the gastrointestinal tract
• c-ErbB3 is overexpressed in a variety of cancers, including breast, ovary, and gastrointestinal carcinomas
• Upon binding its ligand heregulin beta-1, c-ErbB3 is activated by heterodimerization with ErbB2 or EGFR1
• Dependent on PI3K signaling, c-ErbB3 mediates tumor cell invasion, intravasation, and metastasis
• Expression of this cell surface receptor has been used as a prognostic marker for breast cancer
• The SGP1 antibody has been reported to stimulate anchorage-independent growth of breast cancer cell lines
• Applications Reported: This SGP1 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This SGP1 antibody has been pre-titrated and tested by flow cytometric analysis of the MCF7 cell line
• This can be used at 5 μL (0.125 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• PerCP-eFluor™ 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5
• PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm)
• ErbB3 (c-erbB-3/HER-3) is a member of the type I family of growth factor receptors and binds to ligands in the heregulin family
• ErbB3 is over-expressed in a variety of tumors in the prostate, bladder, breast, stomach, pancreas, and colon
• Heregulin and EGF stimulate tyrosine phosphorylation of c-erbB-3 to different extents
• The ErbB3 gene is located on the long arm of human chromosome 12 (12q13) and is transcribed into a 6
• 2kb mRNA which is translated into a 160/180 kDa glycoprotein
• The ErbB3 gene encodes a transmembrane receptor tyrosine kinase due to alternative splicing and forms heterodimers with other EGF receptor family members that have kinase activity
• Heterodimerization leads to the activation of pathways that lead to cell proliferation or differentiation
• Alternate transcriptional splice variants encoding different isoforms of ErbB3 have been characterized
• One ErbB3 isoform lacks the intermembrane region, is secreted outside the cell, and acts to modulate the activity of the membrane-bound form
• Additional splice variants have also been reported, but they have not been thoroughly characterized.