Aiolos Monoclonal Antibody (8B2), eFluor™ 660, eBioscience™, Invitrogen™

Description

• This 8B2 MAb reacts with mouse Aiolos, a 58-kDa member of the Ikaros family of zinc finger transcription factors
• Expression of Aiolos is restricted to the lymphoid lineage, with B cells exhibiting the highest levels of expression, followed by NK and T cells
• Studies demonstrate that Aiolos forms homodimers or heterodimerizes with Ikaros proteins
• Moreover, Aiolos exhibits tyrosine phosphorylation and nuclear localization in response to IL-2 stimulation in T cells
• Aiolos plays a role in IL-2-mediated anti-apoptosis by controlling Bcl-2 expression
• Finally, Aiolos is involved in B cell development, with upregulation of this transcription factor observed at the pre-BI to pre-BII transition in response to pre-B cell receptor signaling
• This gene encodes a member of the Ikaros family of zinc-finger proteins
• Three members of this protein family (Ikaros, Aiolos and Helios) are hematopoietic-specific transcription factors involved in the regulation of lymphocyte development
• This gene product is a transcription factor that is important in the regulation of B lymphocyte proliferation and differentiation
• Both Ikaros and Aiolos can participate in chromatin remodeling
• Regulation of gene expression in B lymphocytes by Aiolos is complex as it appears to require the sequential formation of Ikaros homodimers, Ikaros/Aiolos heterodimers, and Aiolos homodimers
• At least six alternative transcripts encoding different isoforms have been described.