Ly-49G2 Monoclonal Antibody (eBio4D11 (4D11)), FITC, eBioscience™, Invitrogen™

Description

• Description: The 4D11 monoclonal antibody reacts with Ly-49G2 (also known as LGL-1)
• The Ly-49 family of NK-cell receptors, members of the C-type lectin superfamily, are disulfide-linked type-II transmembrane protein homodimers with extracellular carbohydrate recognition domains, which bind to MHC class I alloantigens
• The Ly-49 family members are expressed independently, such that an individual NK or T cell may display more than one class of Ly-49 receptor
• Ly-49G2 is an inhibitory receptor expressed on subsets of natural killer (NK) cells, NK-T cells (in all strains tested), and on a population of memory CD8+ T cells (in C57BL/6 mice)
• The 4D11 antibody has been reported to cross react with the Ly-49AB6, Ly-49ABALB, and Ly-49T129/J inhibitory receptors and Ly-49LCBA/J activatingreceptors
• The proportion of NK-T cells expressing Ly-49A and Ly-49G2 is higher in the thymus than in liver, and there is evidence that down-regulation of Ly-49 receptor expression is necessary for normal NK-T-cell development to occur
• Applications Reported: This eBio4D11 (4D11) antibody has been reported for use in flow cytometric analysis
• Applications Tested: This eBio4D11 (4D11) antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Mouse Ly49 receptors exhibit allelic specificity for MHC class I Ia molecules and are thought to serve to prevent natural killer (NK) cells from attacking normal cells, while allowing them to attack infected or transformed cells in which class I molecules have been down-regulated
• These inhibitory receptors are also expressed on subpopulations of mouse CD8+ T cells.