CCL5 (RANTES) Monoclonal Antibody (VL1), eFluor™ 660, eBioscience™, Invitrogen™

Description

• Description: The VL1 monoclonal antibody reacts with human CCL5/RANTES (Regulated upon Activation, Normal T cell Expressed and Secreted)
• RANTES is an 8 kDa member of the C-C chemokine family
• Although rapidly induced in fibroblasts and epithelial cells, RANTES is upregulated only after 3-5 days of activation in T cells
• As with other members of the C-C chemokine family, RANTES is a strong chemotractant for monocytes, NK cells, eosinophils and T cells
• RANTES is also expressed by monocytes upon activation and has recently been reported to be constitutively expressed by NK cells
• Applications Reported: This VL1 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
• Applications Tested: This VL1 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells
• This can be used at 5 μL (0.25 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• eFluor™ 660 is a replacement for Alexa Fluor™ 647
• eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm)
• Please make sure that your instrument is capable of detecting this fluorochome
• Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser
• Filtration: 0.2 μm post-manufacturing filtered
• CCL5 (RANTES) is a chemokine gene located on the q-arm of chromosome 17
• Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes
• The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide
• CCL5, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells, and eosinophils
• It causes the release of histamine from basophils and activates eosinophils
• This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells
• It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor
• Alternative splicing results in multiple transcript variants that encode different isoforms
• Diseases associated with CCL5 include Human Immunodeficiency Virus Type 1 and Periapical Granuloma.