CD326 (EpCAM) Monoclonal Antibody (1B7), eFluor™ 660, eBioscience™, Invitrogen™

Description

• Description: EpCAM (Epithelial cell adhesion molecule, CD326, KSA, TROP1) is a 40 kD cell-surface adhesion molecule participating in homophilic, calcium-independent cell-cell interactions
• EpCAM is a type-I transmembrane protein, and is expressed primarily on the basolateral surface of most epithelia
• Although normal epithelia express low levels of EpCAM, increased expression has been correlated with increased proliferation and progression to a mesenchymal phenotype
• EpCAM has also been used as a diagnostic marker on circulating metastatic carcinoma cells, while cancer cells of non-epithelial origin do not express EpCAM
• Applications Reported: This 1B7 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 1B7 antibody has been pre-titrated and tested by flow cytometric analysis of human A549 cell line
• This can be used at 5 μL (0.06 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• eFluor™ 660 is a replacement for Alexa Fluor™ 647
• eFluor™ 660 emits at 659 nm and is excited with the red laser (633 nm)
• Please make sure that your instrument is capable of detecting this fluorochome
• Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser
• Filtration: 0.2 μm post-manufacturing filtered
• Ep-CAM (epithelial adhesion molecule, epithelial specific antigen, ESA) is a transmembrane glycoprotein expressed in the epithelium with a molecular weight of approximately 40 kDa, which functions as an epithelial cell adhesion molecule
• Ep-CAM functions as a homotypic calcium-independent cell adhesion molecule, and has a direct impact on cell cycle, proliferation and metabolism of epithelial cells and fibroblasts due to its ability to rapidly induce the proto-oncogene c-myc and the cell cycle regulating genes cyclin A and E
• Ep-CAM mediates Ca2+-independent homotypic interactions
• Formation of Ep-CAM-mediated adhesions have a negative regulatory effect on adhesions mediated by classic cadherins, which may have strong effects on the differentiation and growth of epithelial cells
• Ep-CAM overexpression was suggested to be associated with enhanced epithelial proliferation
• Ep-CAM is highly expressed in human carcinomas, and is a marker for tumors of epithelial lineage
• Ep-CAM is expressed on baso-lateral cell surface in most simple epithelia and many carcinoma types
• Also, Ep-CAM reportedly distinguishes adenocarcinomas from pleural mesotheliomas.