Applications:
Western Blot, ChIP Assay, Flow Cytometry, ELISA, Immunohistochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, In Situ Hybridization (ISH)
Research Discipline:
Autophagy, Cancer, Cellular Markers, Cytoskeleton Markers, Hypoxia, Macroautophagy, Signal Transduction
Formulation:
PBS with 0.02% Sodium Azide
Gene Alias:
ATG8E, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, LC3, LC3A, lc3-i, ii, MAP1A/1B light chain 3 A, MAP1ALC3MAP1A/MAP1B LC3 A, MAP1BLC3MAP1A/MAP1B light chain 3 A, microtubule-associated protein 1 light chain 3 alphaMAP1 light chain 3-like protein 1, microtubule-associated proteins 1A/1B light chain 3, microtubule-associated proteins 1A/1B light chain 3A
Purification Method:
Affinity Purified
Test Specificity:
This antibody detects both LC3A and LC3B.
Dilution:
Western Blot 2.0 ug/ml, Simple Western 1:50, Chromatin Immunoprecipitation reported in scientific literature (PMID 33035707), Flow Cytometry reported in scientific literature (PMID 24419333), ELISA reported in scientific literature (PMID 20930550), Immunohistochemistry 1:200-1:400, Immunocytochemistry/Immunofluorescence 1:100-1:300. Use reported in scientific literature (PMID 21545732), Immunoprecipitation 20 ug/ 500 ug of lysate, Immunohistochemistry-Paraffin 1:200-1:400. Use reported in scientific literature (PMID 26571030), Immunohistochemistry-Frozen, Immunoblotting reported in scientific literature (PMID 28253371), Southern Blot, Immunohistochemistry (Whole-Mount) reported in scientific literature (PMID 31783118), Chromatin Immunoprecipitation (ChIP)
Classification:
Polyclonal
Target Species:
Human, Mouse, Rat, Amphibian, Canine, Fish, Plant, Zebrafish
Gene Accession No.:
Q9GZQ8
Immunogen:
A synthetic peptide made to an internal portion of the human LC3 protein sequence (between residues 25-121). [Uniprot: Q9H492].
Primary or Secondary:
Primary
Content And Storage:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.