LC3A Antibody - BSA Free, Novus Biologicals™
Rabbit Polyclonal Antibody has been used in 265 publications
Manufacturer: Fischer Scientific
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Antigen
LC3A
Dilution
Western Blot 2.0 ug/ml, Simple Western 1:50, Chromatin Immunoprecipitation reported in scientific literature (PMID 33035707), Flow Cytometry reported in scientific literature (PMID 24419333), ELISA reported in scientific literature (PMID 20930550), Immunohistochemistry 1:200-1:400, Immunocytochemistry/Immunofluorescence 1:100-1:300. Use reported in scientific literature (PMID 21545732), Immunoprecipitation 20 ug/ 500 ug of lysate, Immunohistochemistry-Paraffin 1:200-1:400. Use reported in scientific literature (PMID 26571030), Immunohistochemistry-Frozen, Immunoblotting reported in scientific literature (PMID 28253371), Southern Blot, Immunohistochemistry (Whole-Mount) reported in scientific literature (PMID 31783118), Chromatin Immunoprecipitation (ChIP)
Classification
Polyclonal
Host Species
Rabbit
Research Discipline
Autophagy, Cancer, Cellular Markers, Cytoskeleton Markers, Hypoxia, Macroautophagy, Signal Transduction
Formulation
PBS with 0.02% Sodium Azide
Gene Alias
ATG8E, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, LC3, LC3A, lc3-i, ii, MAP1A/1B light chain 3 A, MAP1ALC3MAP1A/MAP1B LC3 A, MAP1BLC3MAP1A/MAP1B light chain 3 A, microtubule-associated protein 1 light chain 3 alphaMAP1 light chain 3-like protein 1, microtubule-associated proteins 1A/1B light chain 3, microtubule-associated proteins 1A/1B light chain 3A
Gene Symbols
MAP1LC3A
Isotype
IgG
Purification Method
Affinity Purified
Test Specificity
This antibody detects both LC3A and LC3B.
Concentration
1.0 mg/mL
Applications
Western Blot, ChIP Assay, Flow Cytometry, ELISA, Immunohistochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, In Situ Hybridization (ISH)
Conjugate
Unconjugated
Regulatory Status
RUO
Target Species
Human, Mouse, Rat, Amphibian, Canine, Fish, Plant, Zebrafish
Gene Accession No.
Q9GZQ8
Gene ID (Entrez)
84557
Immunogen
A synthetic peptide made to an internal portion of the human LC3 protein sequence (between residues 25-121). [Uniprot: Q9H492].
Primary or Secondary
Primary
Content And Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Related Products
Description
- LC3A Polyclonal specifically detects LC3A in Human, Mouse, Rat, Amphibian, Canine, Fish, Plant, Zebrafish samples
- It is validated for Western Blot, Simple Western, Chromatin Immunoprecipitation, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunoblotting, Southern Blot, Immunohistochemistry Whole-Mount.
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Antigen: LC3A
Dilution: Western Blot 2.0 ug/ml, Simple Western 1:50, Chromatin Immunoprecipitation reported in scientific literature (PMID 33035707), Flow Cytometry reported in scientific literature (PMID 24419333), ELISA reported in scientific literature (PMID 20930550), Immunohistochemistry 1:200-1:400, Immunocytochemistry/Immunofluorescence 1:100-1:300. Use reported in scientific literature (PMID 21545732), Immunoprecipitation 20 ug/ 500 ug of lysate, Immunohistochemistry-Paraffin 1:200-1:400. Use reported in scientific literature (PMID 26571030), Immunohistochemistry-Frozen, Immunoblotting reported in scientific literature (PMID 28253371), Southern Blot, Immunohistochemistry (Whole-Mount) reported in scientific literature (PMID 31783118), Chromatin Immunoprecipitation (ChIP)
Classification: Polyclonal
Host Species: Rabbit
Research Discipline: Autophagy, Cancer, Cellular Markers, Cytoskeleton Markers, Hypoxia, Macroautophagy, Signal Transduction
Formulation: PBS with 0.02% Sodium Azide
Gene Alias: ATG8E, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, LC3, LC3A, lc3-i, ii, MAP1A/1B light chain 3 A, MAP1ALC3MAP1A/MAP1B LC3 A, MAP1BLC3MAP1A/MAP1B light chain 3 A, microtubule-associated protein 1 light chain 3 alphaMAP1 light chain 3-like protein 1, microtubule-associated proteins 1A/1B light chain 3, microtubule-associated proteins 1A/1B light chain 3A
Gene Symbols: MAP1LC3A
Isotype: IgG
Purification Method: Affinity Purified
Test Specificity: This antibody detects both LC3A and LC3B.
Concentration: 1.0 mg/mL
Applications: Western Blot, ChIP Assay, Flow Cytometry, ELISA, Immunohistochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, In Situ Hybridization (ISH)
Conjugate: Unconjugated
Regulatory Status: RUO
Target Species: Human, Mouse, Rat, Amphibian, Canine, Fish, Plant, Zebrafish
Gene Accession No.: Q9GZQ8
Gene ID (Entrez): 84557
Immunogen: A synthetic peptide made to an internal portion of the human LC3 protein sequence (between residues 25-121). [Uniprot: Q9H492].
Primary or Secondary: Primary
Content And Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Antigen: LC3A
Dilution: Western Blot 1:1000, Immunohistochemistry 1:400, Immunocytochemistry/Immunofluorescence 1:75, Immunohistochemistry-Paraffin 1:400
Classification: Polyclonal
Host Species: Rabbit
Research Discipline: Macroautophagy
Formulation: PBS and 30% Glycerol with 0.05% Sodium Azide
Gene Alias: ATG8E, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, LC3, LC3A, lc3-i, ii, MAP1A/1B light chain 3 A, MAP1ALC3MAP1A/MAP1B LC3 A, MAP1BLC3MAP1A/MAP1B light chain 3 A, microtubule-associated protein 1 light chain 3 alphaMAP1 light chain 3-like protein 1, microtubule-associated proteins 1A/1B light chain 3, microtubule-associated proteins 1A/1B light chain 3A
Gene Symbols: MAP1LC3B
Isotype: IgG
Purification Method: Affinity Purified
Test Specificity: This LC3I antibody specifically detects the cytosolic form of LC3 before it gets converted into LC3II during autophagy.
Concentration: 1.09 mg/mL
Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Regulatory Status: RUO
Target Species: Human, Mouse, Rat, Porcine, Bovine, Primate
Gene Accession No.: Q9H492
Gene ID (Entrez): 84557
Immunogen: A synthetic peptide made to a C-terminal region of the human LC3 protein (within residues 50-120). [Swiss-Prot Q9H492]
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Antigen: LC3B
Dilution: Western Blot 0.5 - 2.0 ug/mL, Simple Western 1:50, Flow Cytometry, ELISA, Immunohistochemistry 1:200 - 1:400, Immunocytochemistry/Immunofluorescence 1:200, Immunoprecipitation 20 ug/500 ug of protein, Immunohistochemistry-Paraffin 1:200 - 1:400, Immunohistochemistry-Frozen, Immunoblotting, Proximity Ligation Assay, SDS-Page, Chromatin Immunoprecipitation (ChIP), Knockout Validated, KnockDown Validated
Classification: Polyclonal
Host Species: Rabbit
Research Discipline: Autophagy, Cancer, Cardiovascular Biology, Cellular Markers, Hypoxia, Innate Immunity, Lipid and Metabolism, Macroautophagy, Mitophagy, Neuroscience
Formulation: PBS with 0.02% Sodium Azide
Gene Alias: anti-LC3B, ATG8F, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, LC3B, lc3b autophagy marker, LC3B ihc, LC3B immunohistochemistry, LC3B immunoprecipitation, LC3B western blot, lc3-i, ii, MAP1 light chain 3-like protein 2, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, map1lc3b, MAP1LC3B-a, microtubule associated protein 1 light chain 3 beta, microtubule associated protein 3 b, microtubule associated protein 3 beta, microtubule-associated proteins 1A/1B light chain 3, microtubule-associated proteins 1A/1B light chain 3B
Gene Symbols: MAP1LC3B
Isotype: IgG
Purification Method: Affinity Purified
Test Specificity: Human, rat, mouse, zebrafish, bacteria, hamster, and porcine. Bovine reactivity reported in scientific literature (PMID: 24895572) Primate reactivity reported in scientific literature (PMID: 25142602) The mouse detection has been reported to be weaker than the human. Immunogen sequence has 84% homology to Xenopus.
Concentration: 1.0 mg/mL
Applications: ChIP Assay, ELISA, Flow Cytometry, Immunoassay, Immunoblot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, In situ PLA, KnockDown
Conjugate: Unconjugated
Regulatory Status: RUO
Target Species: Human, Mouse, Rat, Porcine, Alligator, Avian, Bacteria, Bovine, Canine, Chicken, Guinea Pig, Hamster, Invertebrate, Monkey, Primate, Rabbit, Golden Syrian Hamster, Zebrafish
Gene Accession No.: Q9GZQ8
Gene ID (Entrez): 81631
Immunogen: Polyclonal LC3B Antibody was made to a synthetic peptide made to an N-terminal portion of the human LC3B protein sequence (between residues 1-100). [UniProt# Q9GZQ8]
Primary or Secondary: Primary
Content And Storage: Store at -20°C.
