5078351

LC3A Antibody - BSA Free, Novus Biologicals™

Rabbit Polyclonal Antibody

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

LC3A

Dilution

Western Blot 1:1000, Immunohistochemistry 1:400, Immunocytochemistry/Immunofluorescence 1:75, Immunohistochemistry-Paraffin 1:400

Classification

Polyclonal

Host Species

Rabbit

Research Discipline

Macroautophagy

Formulation

PBS and 30% Glycerol with 0.05% Sodium Azide

Gene Alias

ATG8E, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, LC3, LC3A, lc3-i, ii, MAP1A/1B light chain 3 A, MAP1ALC3MAP1A/MAP1B LC3 A, MAP1BLC3MAP1A/MAP1B light chain 3 A, microtubule-associated protein 1 light chain 3 alphaMAP1 light chain 3-like protein 1, microtubule-associated proteins 1A/1B light chain 3, microtubule-associated proteins 1A/1B light chain 3A

Gene Symbols

MAP1LC3B

Isotype

IgG

Purification Method

Affinity Purified

Test Specificity

This LC3I antibody specifically detects the cytosolic form of LC3 before it gets converted into LC3II during autophagy.

Concentration

1.09 mg/mL

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)

Conjugate

Unconjugated

Regulatory Status

RUO

Target Species

Human, Mouse, Rat, Porcine, Bovine, Primate

Gene Accession No.

Q9H492

Gene ID (Entrez)

84557

Immunogen

A synthetic peptide made to a C-terminal region of the human LC3 protein (within residues 50-120). [Swiss-Prot Q9H492]

Primary or Secondary

Primary

Content And Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

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Description

  • LC3A Polyclonal specifically detects LC3A in Human, Mouse, Rat, Porcine, Bovine, Primate samples
  • It is validated for Western Blot, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

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Fischer Scientific

5078351

Rabbit Polyclonal Antibody...


Antigen:
LC3A

Dilution:
Western Blot 1:1000, Immunohistochemistry 1:400, Immunocytochemistry/Immunofluorescence 1:75, Immunohistochemistry-Paraffin 1:400

Classification:
Polyclonal

Host Species:
Rabbit

Research Discipline:
Macroautophagy

Formulation:
PBS and 30% Glycerol with 0.05% Sodium Azide

Gene Alias:
ATG8E, Autophagy-related protein LC3 A, Autophagy-related ubiquitin-like modifier LC3 A, LC3, LC3A, lc3-i, ii, MAP1A/1B light chain 3 A, MAP1ALC3MAP1A/MAP1B LC3 A, MAP1BLC3MAP1A/MAP1B light chain 3 A, microtubule-associated protein 1 light chain 3 alphaMAP1 light chain 3-like protein 1, microtubule-associated proteins 1A/1B light chain 3, microtubule-associated proteins 1A/1B light chain 3A

Gene Symbols:
MAP1LC3B

Isotype:
IgG

Purification Method:
Affinity Purified

Test Specificity:
This LC3I antibody specifically detects the cytosolic form of LC3 before it gets converted into LC3II during autophagy.

Concentration:
1.09 mg/mL

Applications:
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Regulatory Status:
RUO

Target Species:
Human, Mouse, Rat, Porcine, Bovine, Primate

Gene Accession No.:
Q9H492

Gene ID (Entrez):
84557

Immunogen:
A synthetic peptide made to a C-terminal region of the human LC3 protein (within residues 50-120). [Swiss-Prot Q9H492]

Primary or Secondary:
Primary

Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

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5078398

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Antigen:
LC3B

Dilution:
Western Blot 0.5 - 2.0 ug/mL, Simple Western 1:50, Flow Cytometry, ELISA, Immunohistochemistry 1:200 - 1:400, Immunocytochemistry/Immunofluorescence 1:200, Immunoprecipitation 20 ug/500 ug of protein, Immunohistochemistry-Paraffin 1:200 - 1:400, Immunohistochemistry-Frozen, Immunoblotting, Proximity Ligation Assay, SDS-Page, Chromatin Immunoprecipitation (ChIP), Knockout Validated, KnockDown Validated

Classification:
Polyclonal

Host Species:
Rabbit

Research Discipline:
Autophagy, Cancer, Cardiovascular Biology, Cellular Markers, Hypoxia, Innate Immunity, Lipid and Metabolism, Macroautophagy, Mitophagy, Neuroscience

Formulation:
PBS with 0.02% Sodium Azide

Gene Alias:
anti-LC3B, ATG8F, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, LC3B, lc3b autophagy marker, LC3B ihc, LC3B immunohistochemistry, LC3B immunoprecipitation, LC3B western blot, lc3-i, ii, MAP1 light chain 3-like protein 2, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, map1lc3b, MAP1LC3B-a, microtubule associated protein 1 light chain 3 beta, microtubule associated protein 3 b, microtubule associated protein 3 beta, microtubule-associated proteins 1A/1B light chain 3, microtubule-associated proteins 1A/1B light chain 3B

Gene Symbols:
MAP1LC3B

Isotype:
IgG

Purification Method:
Affinity Purified

Test Specificity:
Human, rat, mouse, zebrafish, bacteria, hamster, and porcine. Bovine reactivity reported in scientific literature (PMID: 24895572) Primate reactivity reported in scientific literature (PMID: 25142602) The mouse detection has been reported to be weaker than the human. Immunogen sequence has 84% homology to Xenopus.

Concentration:
1.0 mg/mL

Applications:
ChIP Assay, ELISA, Flow Cytometry, Immunoassay, Immunoblot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, In situ PLA, KnockDown

Conjugate:
Unconjugated

Regulatory Status:
RUO

Target Species:
Human, Mouse, Rat, Porcine, Alligator, Avian, Bacteria, Bovine, Canine, Chicken, Guinea Pig, Hamster, Invertebrate, Monkey, Primate, Rabbit, Golden Syrian Hamster, Zebrafish

Gene Accession No.:
Q9GZQ8

Gene ID (Entrez):
81631

Immunogen:
Polyclonal LC3B Antibody was made to a synthetic peptide made to an N-terminal portion of the human LC3B protein sequence (between residues 1-100). [UniProt# Q9GZQ8]

Primary or Secondary:
Primary

Content And Storage:
Store at -20°C.

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5078510

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Antigen:
LCAT

Dilution:
Western Blot 1:1000, ELISA 1:500

Classification:
Polyclonal

Host Species:
Rabbit

Research Discipline:
Cancer, Cholesterol Metabolism, Lipid and Metabolism, Signal Transduction

Formulation:
Whole antisera with 0.05% Sodium Azide

Gene Alias:
EC 2.3.1.43, lecithin-cholesterol acyltransferasePhospholipid-cholesterol acyltransferase, phosphatidylcholine-sterol acyltransferase

Gene Symbols:
LCAT

Isotype:
IgG

Purification Method:
Unpurified

Test Specificity:
__

Concentration:
__

Applications:
Western Blot, ELISA

Conjugate:
Unconjugated

Regulatory Status:
RUO

Target Species:
Human, Mouse

Gene Accession No.:
P04180

Gene ID (Entrez):
3931

Immunogen:
Carboxyl-terminal histidine-tagged human recombinant LCAT purified from the medium of CHO cells overexpressing human LCAT cDNA. [UniProt# P04180]

Primary or Secondary:
Primary

Content And Storage:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

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5078513

Rabbit Polyclonal Antibody has been...


Antigen:
LCAT

Dilution:
Western Blot 1:1000, Immunocytochemistry/Immunofluorescence 1:500

Classification:
Polyclonal

Host Species:
Rabbit

Research Discipline:
Cancer, Cholesterol Metabolism, Lipid and Metabolism, Signal Transduction

Formulation:
Whole antisera with 0.05% Sodium Azide

Gene Alias:
EC 2.3.1.43, lecithin-cholesterol acyltransferasePhospholipid-cholesterol acyltransferase, phosphatidylcholine-sterol acyltransferase

Gene Symbols:
LCAT

Isotype:
IgG

Purification Method:
Unpurified

Test Specificity:
__

Concentration:
__

Applications:
Western Blot, Immunocytochemistry, Immunofluorescence

Conjugate:
Unconjugated

Regulatory Status:
RUO

Target Species:
Human, Mouse

Gene Accession No.:
P04180

Gene ID (Entrez):
3931

Immunogen:
Carboxyl-terminal histidine-tagged mouse recombinant LCAT purified from the medium of CHO cells overexpressing mouse LCAT cDNA. [UniProt# P16301]

Primary or Secondary:
Primary

Content And Storage:
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.