5092474
SMAC/Diablo Antibody, Novus Biologicals™
Rabbit Polyclonal Antibody has been used in 16 publications
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
SMAC/Diablo
Dilution
Western Blot 0.5-2 ug/ml, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:10-1:500. Use reported in scientific literature (Hu et al (2004)), Immunohistochemistry-Paraffin 10 ug/ml
Classification
Polyclonal
Form
Purified
Regulatory Status
RUO
Formulation
PBS with 0.02% Sodium Azide
Gene Alias
0610041G12Rik, diablo, IAP-binding mitochondrial protein, Direct IAP-binding protein with low pI, mitochondrial Smac protein, Second mitochondria-derived activator of caspase, Smac, SMACmitochondrial
Gene Symbols
DIABLO
Isotype
IgG
Purification Method
Protein G purified
Test Specificity
Smac
Concentration
0.5 mg/mL
Applications
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Conjugate
Unconjugated
Host Species
Rabbit
Target Species
Human
Gene Accession No.
Q9NR28
Gene ID (Entrez)
56616
Immunogen
This rabbit polyclonal antibody was developed against two synthetic peptides corresponding to amino acids 199 to 212 and amino acids 226 to 239 of human Smac (Genbank accession no. AAF87716).
Primary or Secondary
Primary
Content And Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Description
- SMAC/Diablo Polyclonal specifically detects SMAC/Diablo in Human, Mouse samples
- It is validated for Western Blot, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.
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Antigen: SMAC/Diablo
Dilution: Western Blot 0.5-2 ug/ml, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:10-1:500. Use reported in scientific literature (Hu et al (2004)), Immunohistochemistry-Paraffin 10 ug/ml
Classification: Polyclonal
Form: Purified
Regulatory Status: RUO
Formulation: PBS with 0.02% Sodium Azide
Gene Alias: 0610041G12Rik, diablo, IAP-binding mitochondrial protein, Direct IAP-binding protein with low pI, mitochondrial Smac protein, Second mitochondria-derived activator of caspase, Smac, SMACmitochondrial
Gene Symbols: DIABLO
Isotype: IgG
Purification Method: Protein G purified
Test Specificity: Smac
Concentration: 0.5 mg/mL
Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Host Species: Rabbit
Target Species: Human
Gene Accession No.: Q9NR28
Gene ID (Entrez): 56616
Immunogen: This rabbit polyclonal antibody was developed against two synthetic peptides corresponding to amino acids 199 to 212 and amino acids 226 to 239 of human Smac (Genbank accession no. AAF87716).
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Antigen:
SMAC/Diablo
Dilution:
Western Blot 0.5-2 ug/ml, Immunohistochemistry, Immunocytochemistry/Immunofluorescence 1:10-1:500. Use reported in scientific literature (Hu et al (2004)), Immunohistochemistry-Paraffin 10 ug/ml
Classification:
Polyclonal
Form:
Purified
Regulatory Status:
RUO
Formulation:
PBS with 0.02% Sodium Azide
Gene Alias:
0610041G12Rik, diablo, IAP-binding mitochondrial protein, Direct IAP-binding protein with low pI, mitochondrial Smac protein, Second mitochondria-derived activator of caspase, Smac, SMACmitochondrial
Gene Symbols:
DIABLO
Isotype:
IgG
Purification Method:
Protein G purified
Test Specificity:
Smac
Concentration:
0.5 mg/mL
Applications:
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Host Species:
Rabbit
Target Species:
Human
Gene Accession No.:
Q9NR28
Gene ID (Entrez):
56616
Immunogen:
This rabbit polyclonal antibody was developed against two synthetic peptides corresponding to amino acids 199 to 212 and amino acids 226 to 239 of human Smac (Genbank accession no. AAF87716).
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Antigen: SMAD1/5/8/9
Dilution: Western Blot 0.5 - 2 ug/ml
Classification: Polyclonal
Form: Purified
Regulatory Status: RUO
Formulation: 0.1 ml PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias: BSP1, BSP-1, hSMAD1, JV4-1SMAD, mothers against DPP homolog 1 (Drosophila), MAD homolog 1, MADH1JV41, MADR1MAD, mothers against decapentaplegic homolog 1 (Drosophila), Mad-related protein 1, mothers against decapentaplegic homolog 1, Mothers against DPP homolog 1, SMAD family member 1SMAD 1, SMAD, mothers against DPP homolog 1, Smad1, TGF-beta signaling protein 1, transforming growth factor-beta signaling protein 1
Gene Symbols: SMAD1
Isotype: IgG
Purification Method: Protein G purified
Test Specificity: The amino acid sequences used to make this antibody are 100% conserved between SMAD1/5/8/9. Therefore, the antibody recognizes SMADs 1, 5, 8, and 9. The antibody may cross-react with SMAD2 or 3. The actual SMADs detected with this antibody may vary according to cell or tissue-type specific expression.
Concentration: 0.5 mg/mL
Applications: Western Blot
Conjugate: Unconjugated
Host Species: Rabbit
Target Species: Human, Mouse, Rat
Gene Accession No.: __
Gene ID (Entrez): 4086
Immunogen: This antibody was raised against two synthetic peptides corresponding to amino acids 18-32 (LGWKQGDEEEKWAEK) and amino acids 315-329 (SNVNRNSTIENTRRH) of human SMAD1. GenBank Accession No. AAP36050.1. They are 100% conserved or highly conserved between human and multiple species.
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Antigen:
SMAD1/5/8/9
Dilution:
Western Blot 0.5 - 2 ug/ml
Classification:
Polyclonal
Form:
Purified
Regulatory Status:
RUO
Formulation:
0.1 ml PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Alias:
BSP1, BSP-1, hSMAD1, JV4-1SMAD, mothers against DPP homolog 1 (Drosophila), MAD homolog 1, MADH1JV41, MADR1MAD, mothers against decapentaplegic homolog 1 (Drosophila), Mad-related protein 1, mothers against decapentaplegic homolog 1, Mothers against DPP homolog 1, SMAD family member 1SMAD 1, SMAD, mothers against DPP homolog 1, Smad1, TGF-beta signaling protein 1, transforming growth factor-beta signaling protein 1
Gene Symbols:
SMAD1
Isotype:
IgG
Purification Method:
Protein G purified
Test Specificity:
The amino acid sequences used to make this antibody are 100% conserved between SMAD1/5/8/9. Therefore, the antibody recognizes SMADs 1, 5, 8, and 9. The antibody may cross-react with SMAD2 or 3. The actual SMADs detected with this antibody may vary according to cell or tissue-type specific expression.
Concentration:
0.5 mg/mL
Applications:
Western Blot
Conjugate:
Unconjugated
Host Species:
Rabbit
Target Species:
Human, Mouse, Rat
Gene Accession No.:
__
Gene ID (Entrez):
4086
Immunogen:
This antibody was raised against two synthetic peptides corresponding to amino acids 18-32 (LGWKQGDEEEKWAEK) and amino acids 315-329 (SNVNRNSTIENTRRH) of human SMAD1. GenBank Accession No. AAP36050.1. They are 100% conserved or highly conserved between human and multiple species.
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Antigen: Smad2
Dilution: Western Blot 2-4 ug/ml, Immunohistochemistry-Paraffin 5 ug/ml
Classification: Polyclonal
Form: Purified
Regulatory Status: RUO
Formulation: PBS with 0.05% Sodium Azide
Gene Alias: hMAD-2, hSMAD2, JV18, JV18-1SMAD, mothers against DPP homolog 2 (Drosophila), MAD homolog 2, Mad protein homolog, Mad, mothers against decapentaplegic homolog 2, MADH2MGC34440, MADR2MGC22139, Mad-related protein 2, mother against DPP homolog 2, mothers against decapentaplegic homolog 2, Mothers against DPP homolog 2, Sma- and Mad-related protein 2, SMAD 2, SMAD family member 2MAD, mothers against decapentaplegic homolog 2 (Drosophila), SMAD, mothers against DPP homolog 2, Smad2
Gene Symbols: SMAD2
Isotype: IgG
Purification Method: Protein G purified
Test Specificity: The SMAD2 amino acid sequence used as immunogen is 100% homologous in human (isoform 1: 467 amino acids; isoform 2: 437 amino acids), mouse, rat, horse, cow, chicken and Xenopus.
Concentration: 1.0 mg/mL
Applications: Western Blot, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Host Species: Rabbit
Target Species: Human, Mouse, Rat, Chicken
Gene Accession No.: NP_005892
Gene ID (Entrez): 4087
Immunogen: Amino acids 234-249 (DQQLNQSMDTGSPAEL) of human SMAD2 protein was used as the immunogen.
Primary or Secondary: Primary
Content And Storage: Store at 4C. Do not freeze.
Antigen:
Smad2
Dilution:
Western Blot 2-4 ug/ml, Immunohistochemistry-Paraffin 5 ug/ml
Classification:
Polyclonal
Form:
Purified
Regulatory Status:
RUO
Formulation:
PBS with 0.05% Sodium Azide
Gene Alias:
hMAD-2, hSMAD2, JV18, JV18-1SMAD, mothers against DPP homolog 2 (Drosophila), MAD homolog 2, Mad protein homolog, Mad, mothers against decapentaplegic homolog 2, MADH2MGC34440, MADR2MGC22139, Mad-related protein 2, mother against DPP homolog 2, mothers against decapentaplegic homolog 2, Mothers against DPP homolog 2, Sma- and Mad-related protein 2, SMAD 2, SMAD family member 2MAD, mothers against decapentaplegic homolog 2 (Drosophila), SMAD, mothers against DPP homolog 2, Smad2
Gene Symbols:
SMAD2
Isotype:
IgG
Purification Method:
Protein G purified
Test Specificity:
The SMAD2 amino acid sequence used as immunogen is 100% homologous in human (isoform 1: 467 amino acids; isoform 2: 437 amino acids), mouse, rat, horse, cow, chicken and Xenopus.
Concentration:
1.0 mg/mL
Applications:
Western Blot, Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Host Species:
Rabbit
Target Species:
Human, Mouse, Rat, Chicken
Gene Accession No.:
NP_005892
Gene ID (Entrez):
4087
Immunogen:
Amino acids 234-249 (DQQLNQSMDTGSPAEL) of human SMAD2 protein was used as the immunogen.
Primary or Secondary:
Primary
Content And Storage:
Store at 4C. Do not freeze.
Antigen: Smad3
Dilution: Western Blot 0.5-2 ug/ml, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin 5 ug/ml
Classification: Polyclonal
Form: Purified
Regulatory Status: RUO
Formulation: PBS with 0.05% Sodium Azide
Gene Alias: DKFZp586N0721, DKFZp686J10186, hMAD-3, hSMAD3, HsT17436, JV15-2MGC60396, MAD homolog 3, mad protein homolog, MAD, mothers against decapentaplegic homolog 3, MAD, mothers against decapentaplegic homolog 3 (Drosophila), mad3, MADH3mad homolog JV15-2, mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA- and MAD-related protein 3, SMAD 3, SMAD family member 3HSPC193, SMAD, mothers against DPP homolog 3, SMAD, mothers against DPP homolog 3 (Drosophila), Smad3
Gene Symbols: SMAD3
Isotype: IgG
Purification Method: Protein G purified
Test Specificity: Cross reacts with Bovine, Dog, Human, Mouse, Pig, Rat and Rhesus Monkey. Predicted to react with Zebrafish, Xenopus and Chicken. The amino acid sequence used as immunogen is 100% homologous in human, mouse, rat, dog, pig, bovine and rhesus monkey, and 90% homologous in chicken, xenopus and zebrafish.
Concentration: 1.0 mg/mL
Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Host Species: Rabbit
Target Species: Human, Mouse, Rat, Porcine, Bovine, Canine, Primate
Gene Accession No.: NP_005893
Gene ID (Entrez): 4088
Immunogen: A portion of amino acids 100-150 of human SMAD3 was used as the immunogen.
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Antigen:
Smad3
Dilution:
Western Blot 0.5-2 ug/ml, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin 5 ug/ml
Classification:
Polyclonal
Form:
Purified
Regulatory Status:
RUO
Formulation:
PBS with 0.05% Sodium Azide
Gene Alias:
DKFZp586N0721, DKFZp686J10186, hMAD-3, hSMAD3, HsT17436, JV15-2MGC60396, MAD homolog 3, mad protein homolog, MAD, mothers against decapentaplegic homolog 3, MAD, mothers against decapentaplegic homolog 3 (Drosophila), mad3, MADH3mad homolog JV15-2, mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA- and MAD-related protein 3, SMAD 3, SMAD family member 3HSPC193, SMAD, mothers against DPP homolog 3, SMAD, mothers against DPP homolog 3 (Drosophila), Smad3
Gene Symbols:
SMAD3
Isotype:
IgG
Purification Method:
Protein G purified
Test Specificity:
Cross reacts with Bovine, Dog, Human, Mouse, Pig, Rat and Rhesus Monkey. Predicted to react with Zebrafish, Xenopus and Chicken. The amino acid sequence used as immunogen is 100% homologous in human, mouse, rat, dog, pig, bovine and rhesus monkey, and 90% homologous in chicken, xenopus and zebrafish.
Concentration:
1.0 mg/mL
Applications:
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Host Species:
Rabbit
Target Species:
Human, Mouse, Rat, Porcine, Bovine, Canine, Primate
Gene Accession No.:
NP_005893
Gene ID (Entrez):
4088
Immunogen:
A portion of amino acids 100-150 of human SMAD3 was used as the immunogen.
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.