5092497
SMAD1/5/8/9 Antibody, Novus Biologicals™
Rabbit Polyclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
SMAD1/5/8/9
Dilution
Western Blot 0.5 - 2 ug/ml
Classification
Polyclonal
Form
Purified
Regulatory Status
RUO
Formulation
0.1 ml PBS and 0.05% BSA with 0.05% Sodium Azide
Gene ID (Entrez)
4086
Immunogen
This antibody was raised against two synthetic peptides corresponding to amino acids 18-32 (LGWKQGDEEEKWAEK) and amino acids 315-329 (SNVNRNSTIENTRRH) of human SMAD1. GenBank Accession No. AAP36050.1. They are 100% conserved or highly conserved between human and multiple species.
Primary or Secondary
Primary
Content And Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration
0.5 mg/mL
Applications
Western Blot
Conjugate
Unconjugated
Host Species
Rabbit
Target Species
Human, Mouse, Rat
Gene Alias
BSP1, BSP-1, hSMAD1, JV4-1SMAD, mothers against DPP homolog 1 (Drosophila), MAD homolog 1, MADH1JV41, MADR1MAD, mothers against decapentaplegic homolog 1 (Drosophila), Mad-related protein 1, mothers against decapentaplegic homolog 1, Mothers against DPP homolog 1, SMAD family member 1SMAD 1, SMAD, mothers against DPP homolog 1, Smad1, TGF-beta signaling protein 1, transforming growth factor-beta signaling protein 1
Gene Symbols
SMAD1
Isotype
IgG
Purification Method
Protein G purified
Test Specificity
The amino acid sequences used to make this antibody are 100% conserved between SMAD1/5/8/9. Therefore, the antibody recognizes SMADs 1, 5, 8, and 9. The antibody may cross-react with SMAD2 or 3. The actual SMADs detected with this antibody may vary according to cell or tissue-type specific expression.
Related Products
Description
- SMAD1/5/8/9 Polyclonal specifically detects SMAD1/5/8/9 in Human, Mouse, Rat samples
- It is validated for Western Blot.
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Antigen: SMAD1/5/8/9
Dilution: Western Blot 0.5 - 2 ug/ml
Classification: Polyclonal
Form: Purified
Regulatory Status: RUO
Formulation: 0.1 ml PBS and 0.05% BSA with 0.05% Sodium Azide
Gene ID (Entrez): 4086
Immunogen: This antibody was raised against two synthetic peptides corresponding to amino acids 18-32 (LGWKQGDEEEKWAEK) and amino acids 315-329 (SNVNRNSTIENTRRH) of human SMAD1. GenBank Accession No. AAP36050.1. They are 100% conserved or highly conserved between human and multiple species.
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration: 0.5 mg/mL
Applications: Western Blot
Conjugate: Unconjugated
Host Species: Rabbit
Target Species: Human, Mouse, Rat
Gene Alias: BSP1, BSP-1, hSMAD1, JV4-1SMAD, mothers against DPP homolog 1 (Drosophila), MAD homolog 1, MADH1JV41, MADR1MAD, mothers against decapentaplegic homolog 1 (Drosophila), Mad-related protein 1, mothers against decapentaplegic homolog 1, Mothers against DPP homolog 1, SMAD family member 1SMAD 1, SMAD, mothers against DPP homolog 1, Smad1, TGF-beta signaling protein 1, transforming growth factor-beta signaling protein 1
Gene Symbols: SMAD1
Isotype: IgG
Purification Method: Protein G purified
Test Specificity: The amino acid sequences used to make this antibody are 100% conserved between SMAD1/5/8/9. Therefore, the antibody recognizes SMADs 1, 5, 8, and 9. The antibody may cross-react with SMAD2 or 3. The actual SMADs detected with this antibody may vary according to cell or tissue-type specific expression.
Antigen:
SMAD1/5/8/9
Dilution:
Western Blot 0.5 - 2 ug/ml
Classification:
Polyclonal
Form:
Purified
Regulatory Status:
RUO
Formulation:
0.1 ml PBS and 0.05% BSA with 0.05% Sodium Azide
Gene ID (Entrez):
4086
Immunogen:
This antibody was raised against two synthetic peptides corresponding to amino acids 18-32 (LGWKQGDEEEKWAEK) and amino acids 315-329 (SNVNRNSTIENTRRH) of human SMAD1. GenBank Accession No. AAP36050.1. They are 100% conserved or highly conserved between human and multiple species.
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration:
0.5 mg/mL
Applications:
Western Blot
Conjugate:
Unconjugated
Host Species:
Rabbit
Target Species:
Human, Mouse, Rat
Gene Alias:
BSP1, BSP-1, hSMAD1, JV4-1SMAD, mothers against DPP homolog 1 (Drosophila), MAD homolog 1, MADH1JV41, MADR1MAD, mothers against decapentaplegic homolog 1 (Drosophila), Mad-related protein 1, mothers against decapentaplegic homolog 1, Mothers against DPP homolog 1, SMAD family member 1SMAD 1, SMAD, mothers against DPP homolog 1, Smad1, TGF-beta signaling protein 1, transforming growth factor-beta signaling protein 1
Gene Symbols:
SMAD1
Isotype:
IgG
Purification Method:
Protein G purified
Test Specificity:
The amino acid sequences used to make this antibody are 100% conserved between SMAD1/5/8/9. Therefore, the antibody recognizes SMADs 1, 5, 8, and 9. The antibody may cross-react with SMAD2 or 3. The actual SMADs detected with this antibody may vary according to cell or tissue-type specific expression.
Antigen: Smad2
Dilution: Western Blot 2-4 ug/ml, Immunohistochemistry-Paraffin 5 ug/ml
Classification: Polyclonal
Form: Purified
Regulatory Status: RUO
Formulation: PBS with 0.05% Sodium Azide
Gene ID (Entrez): 4087
Immunogen: Amino acids 234-249 (DQQLNQSMDTGSPAEL) of human SMAD2 protein was used as the immunogen.
Primary or Secondary: Primary
Content And Storage: Store at 4C. Do not freeze.
Concentration: 1.0 mg/mL
Applications: Western Blot, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Host Species: Rabbit
Target Species: Human, Mouse, Rat, Chicken
Gene Alias: hMAD-2, hSMAD2, JV18, JV18-1SMAD, mothers against DPP homolog 2 (Drosophila), MAD homolog 2, Mad protein homolog, Mad, mothers against decapentaplegic homolog 2, MADH2MGC34440, MADR2MGC22139, Mad-related protein 2, mother against DPP homolog 2, mothers against decapentaplegic homolog 2, Mothers against DPP homolog 2, Sma- and Mad-related protein 2, SMAD 2, SMAD family member 2MAD, mothers against decapentaplegic homolog 2 (Drosophila), SMAD, mothers against DPP homolog 2, Smad2
Gene Symbols: SMAD2
Isotype: IgG
Purification Method: Protein G purified
Test Specificity: The SMAD2 amino acid sequence used as immunogen is 100% homologous in human (isoform 1: 467 amino acids; isoform 2: 437 amino acids), mouse, rat, horse, cow, chicken and Xenopus.
Antigen:
Smad2
Dilution:
Western Blot 2-4 ug/ml, Immunohistochemistry-Paraffin 5 ug/ml
Classification:
Polyclonal
Form:
Purified
Regulatory Status:
RUO
Formulation:
PBS with 0.05% Sodium Azide
Gene ID (Entrez):
4087
Immunogen:
Amino acids 234-249 (DQQLNQSMDTGSPAEL) of human SMAD2 protein was used as the immunogen.
Primary or Secondary:
Primary
Content And Storage:
Store at 4C. Do not freeze.
Concentration:
1.0 mg/mL
Applications:
Western Blot, Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Host Species:
Rabbit
Target Species:
Human, Mouse, Rat, Chicken
Gene Alias:
hMAD-2, hSMAD2, JV18, JV18-1SMAD, mothers against DPP homolog 2 (Drosophila), MAD homolog 2, Mad protein homolog, Mad, mothers against decapentaplegic homolog 2, MADH2MGC34440, MADR2MGC22139, Mad-related protein 2, mother against DPP homolog 2, mothers against decapentaplegic homolog 2, Mothers against DPP homolog 2, Sma- and Mad-related protein 2, SMAD 2, SMAD family member 2MAD, mothers against decapentaplegic homolog 2 (Drosophila), SMAD, mothers against DPP homolog 2, Smad2
Gene Symbols:
SMAD2
Isotype:
IgG
Purification Method:
Protein G purified
Test Specificity:
The SMAD2 amino acid sequence used as immunogen is 100% homologous in human (isoform 1: 467 amino acids; isoform 2: 437 amino acids), mouse, rat, horse, cow, chicken and Xenopus.
Antigen: Smad3
Dilution: Western Blot 0.5-2 ug/ml, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin 5 ug/ml
Classification: Polyclonal
Form: Purified
Regulatory Status: RUO
Formulation: PBS with 0.05% Sodium Azide
Gene ID (Entrez): 4088
Immunogen: A portion of amino acids 100-150 of human SMAD3 was used as the immunogen.
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration: 1.0 mg/mL
Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Host Species: Rabbit
Target Species: Human, Mouse, Rat, Porcine, Bovine, Canine, Primate
Gene Alias: DKFZp586N0721, DKFZp686J10186, hMAD-3, hSMAD3, HsT17436, JV15-2MGC60396, MAD homolog 3, mad protein homolog, MAD, mothers against decapentaplegic homolog 3, MAD, mothers against decapentaplegic homolog 3 (Drosophila), mad3, MADH3mad homolog JV15-2, mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA- and MAD-related protein 3, SMAD 3, SMAD family member 3HSPC193, SMAD, mothers against DPP homolog 3, SMAD, mothers against DPP homolog 3 (Drosophila), Smad3
Gene Symbols: SMAD3
Isotype: IgG
Purification Method: Protein G purified
Test Specificity: Cross reacts with Bovine, Dog, Human, Mouse, Pig, Rat and Rhesus Monkey. Predicted to react with Zebrafish, Xenopus and Chicken. The amino acid sequence used as immunogen is 100% homologous in human, mouse, rat, dog, pig, bovine and rhesus monkey, and 90% homologous in chicken, xenopus and zebrafish.
Antigen:
Smad3
Dilution:
Western Blot 0.5-2 ug/ml, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin 5 ug/ml
Classification:
Polyclonal
Form:
Purified
Regulatory Status:
RUO
Formulation:
PBS with 0.05% Sodium Azide
Gene ID (Entrez):
4088
Immunogen:
A portion of amino acids 100-150 of human SMAD3 was used as the immunogen.
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration:
1.0 mg/mL
Applications:
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Host Species:
Rabbit
Target Species:
Human, Mouse, Rat, Porcine, Bovine, Canine, Primate
Gene Alias:
DKFZp586N0721, DKFZp686J10186, hMAD-3, hSMAD3, HsT17436, JV15-2MGC60396, MAD homolog 3, mad protein homolog, MAD, mothers against decapentaplegic homolog 3, MAD, mothers against decapentaplegic homolog 3 (Drosophila), mad3, MADH3mad homolog JV15-2, mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA- and MAD-related protein 3, SMAD 3, SMAD family member 3HSPC193, SMAD, mothers against DPP homolog 3, SMAD, mothers against DPP homolog 3 (Drosophila), Smad3
Gene Symbols:
SMAD3
Isotype:
IgG
Purification Method:
Protein G purified
Test Specificity:
Cross reacts with Bovine, Dog, Human, Mouse, Pig, Rat and Rhesus Monkey. Predicted to react with Zebrafish, Xenopus and Chicken. The amino acid sequence used as immunogen is 100% homologous in human, mouse, rat, dog, pig, bovine and rhesus monkey, and 90% homologous in chicken, xenopus and zebrafish.
Antigen: Smad3
Dilution: Western Blot 0.5-2 ug/ml, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin 5 ug/ml
Classification: Polyclonal
Form: Purified
Regulatory Status: RUO
Formulation: PBS with 0.05% Sodium Azide
Gene ID (Entrez): 4088
Immunogen: A portion of amino acids 100-150 of human SMAD3 was used as the immunogen.
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration: 1.0 mg/mL
Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Host Species: Rabbit
Target Species: Human, Mouse, Rat, Porcine, Bovine, Canine, Primate
Gene Alias: DKFZp586N0721, DKFZp686J10186, hMAD-3, hSMAD3, HsT17436, JV15-2MGC60396, MAD homolog 3, mad protein homolog, MAD, mothers against decapentaplegic homolog 3, MAD, mothers against decapentaplegic homolog 3 (Drosophila), mad3, MADH3mad homolog JV15-2, mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA- and MAD-related protein 3, SMAD 3, SMAD family member 3HSPC193, SMAD, mothers against DPP homolog 3, SMAD, mothers against DPP homolog 3 (Drosophila), Smad3
Gene Symbols: SMAD3
Isotype: IgG
Purification Method: Protein G purified
Test Specificity: Cross reacts with Bovine, Dog, Human, Mouse, Pig, Rat and Rhesus Monkey. Predicted to react with Zebrafish, Xenopus and Chicken. The amino acid sequence used as immunogen is 100% homologous in human, mouse, rat, dog, pig, bovine and rhesus monkey, and 90% homologous in chicken, xenopus and zebrafish.
Antigen:
Smad3
Dilution:
Western Blot 0.5-2 ug/ml, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin 5 ug/ml
Classification:
Polyclonal
Form:
Purified
Regulatory Status:
RUO
Formulation:
PBS with 0.05% Sodium Azide
Gene ID (Entrez):
4088
Immunogen:
A portion of amino acids 100-150 of human SMAD3 was used as the immunogen.
Primary or Secondary:
Primary
Content And Storage:
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration:
1.0 mg/mL
Applications:
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Host Species:
Rabbit
Target Species:
Human, Mouse, Rat, Porcine, Bovine, Canine, Primate
Gene Alias:
DKFZp586N0721, DKFZp686J10186, hMAD-3, hSMAD3, HsT17436, JV15-2MGC60396, MAD homolog 3, mad protein homolog, MAD, mothers against decapentaplegic homolog 3, MAD, mothers against decapentaplegic homolog 3 (Drosophila), mad3, MADH3mad homolog JV15-2, mothers against decapentaplegic homolog 3, Mothers against DPP homolog 3, SMA- and MAD-related protein 3, SMAD 3, SMAD family member 3HSPC193, SMAD, mothers against DPP homolog 3, SMAD, mothers against DPP homolog 3 (Drosophila), Smad3
Gene Symbols:
SMAD3
Isotype:
IgG
Purification Method:
Protein G purified
Test Specificity:
Cross reacts with Bovine, Dog, Human, Mouse, Pig, Rat and Rhesus Monkey. Predicted to react with Zebrafish, Xenopus and Chicken. The amino acid sequence used as immunogen is 100% homologous in human, mouse, rat, dog, pig, bovine and rhesus monkey, and 90% homologous in chicken, xenopus and zebrafish.