CD204 Monoclonal Antibody (M204PA), Alexa Fluor™ 488, eBioscience™, Invitrogen™

Description

• Description: This M204PA antibody recognizes mouse CD204 also known as Macrophage Scavenger Receptor 1 (MSR 1), Scavenger Receptor A (SR-A) or SCARA1
• CD204 belongs to the class A family of scavenger receptors that are primarily responsible for binding a wide variety of macromolecules and are involved in many aspects of macrophage activity
• Other members of this family include: MARCO (SCARA2), CSR (SCARA3), SRCL (SCARA4) and SCARA5
• All class A scavenger receptors are trans-membrane proteins with extracellular C-termini and homotrimeric structures
• CD204 exists as 2 splice variants: the more ubiquitous full length isoform (type I) and a shorter isoform (type II) that lacks the C-terminal cysteine-rich domain
• Both types are functional and bind modified LDL with similar affinity
• In addition to lipid metabolism, CD204 plays an important role suppressing inflammation and macrophage activation
• CD204 is considered one of the markers of M2 and Tumor-Associated Macrophages
• Interestingly, this molecule is responsible for adherence of RAW264.7 cells to tissue culture-treated plastic in the absence of Ca and Mg cations
• This M204PA antibody binds to CD204 of multiple mouse strains, including C57Bl/6, Balb/c and Swiss-Webster (outbred)
• This antibody works on fixed cells and can be used to stain CD204 intracellularly
• The M204PA antibody does not cross-react with human or rat
• This antibody is not suitable for IHC staining
• CD204 gene encodes the class A macrophage scavenger receptors, which include three different types (1, 2, 3) generated by alternative splicing of this gene
• These receptors or isoforms are macrophage-specific trimeric integral membrane glycoproteins and have been implicated in many macrophage-associated physiological and pathological processes including atherosclerosis, Alzheimer's disease, and host defense
• The isoforms type 1 and type 2 are functional receptors and are able to mediate the endocytosis of modified low density lipoproteins (LDLs)
• The isoform type 3 does not internalize modified LDL (acetyl-LDL) despite having the domain shown to mediate this function in the types 1 and 2 isoforms
• It has an altered intracellular processing and is trapped within the endoplasmic reticulum, making it unable to perform endocytosis
• The isoform type 3 can inhibit the function of isoforms type 1 and type 2 when co-expressed, indicating a dominant negative effect and suggesting a mechanism for regulation of scavenger receptor activity in macrophages
• Diseases associated with MSR1 include Barrett Esophagus and Prostate Cancer.