Smooth Muscle Myosin Monoclonal Antibody (SMMS-1), Alexa Fluor™ 488, eBioscience™, Invitrogen™

Description

• Description: This SMMS-1 monoclonal antibody reacts with both isoforms of the human smooth muscle myosin heavy chain (SM1 and SM2) structural proteins, which are components of the contractile apparatus in smooth muscle cells
• Smooth muscle myosin heavy chains are expressed by myoepithelial cells and also serve as the terminal differentiation marker for smooth muscle cells
• In normal breast tissue, myoepithelial cells compose the outer layer of the lumen lining
• In benign and in situ breast lesions, the intact myoepithelial layer stains with SMMS-1 and the loss of this staining indicates invasive cancer
• This SMMS-1 antibody has also been reported to cross-react with bovine, canine, feline, and rat smooth muscle myosin heavy chains
• Applications Reported: This SMMS-1 antibody has been reported for use in microscopy, immunocytochemistry, immunohistochemical staining of frozen tissue sections, and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections
• Applications Tested: This SMMS-1 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retrieval and can be used at less than or equal to 30 μg/mL
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser
• Filtration: 0.2 μm post-manufacturing filtered
• SM-MHC is a cytoplasmic structural protein, which is a major component of the contractile apparatus in smooth muscle cells
• Expression of smooth muscle myosin is developmently regulated, appearing early in smooth muscle development, and is specific for smooth muscle development
• Two isoforms of smooth muscle myosin heavy chain have been identified, designated MHC-1 and MHC-2.