5349385
MilliporeSigma™ anti-CD45R (B220) Red Fluor 710, Clone: RA3-6B2,
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD45R (B220)
Applications
Flow Cytometry
Conjugate
Red Fluor 710
Host Species
Rat
Research Discipline
Inflammation, Immunology
Formulation
Purified Rat monoclonal containing 10mM NaH2PO4, 150mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2.
Gene Symbols
Ptprc, Ly-5
Primary or Secondary
Primary
Content And Storage
2°C to 8°C undiluted for one year from date of shipment protected from light; Do not freeze
Clone
RA3-6B2
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human, Mouse
Gene Accession No.
P06800
Immunogen
Proprietary immunogen
Purification Method
Purified by size-exclusion chromatography
Description
- Specifically detects CD45R (B220) Clone: RA3-6B2 in Human, Mouse samples, and it is validated for Flow Cytometry The RA3-6B2 antibody reacts with the human and mouse CD45 isoform known as CD45R, or B220, a protein tyrosine phosphatase of 220kDa
- CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA)
- Various isoforms are generated and expressed in cell-specific patterns, all critical for leukocyte function
- In mouse, the CD45R/B220 isoform is predominantly found on B cells, at varying levels on all stages from pro-B cells to activated B cells, and may also be detected on certain T cell and NK cell subsets
- It is of note that B220 is not similarly expressed on human B cells, where it appears to be differentiation-specific and therefore expressed on only some B cell subsets
- Other forms of CD45 with restricted cellular expression include CD45RA, CD45RB, CD45RO and several others.The RA3-6B2 antibody is one of the most consistently used leukocyte markers for B cells, T cell subsets and NK cell subsets in human and mouse
- This antibody has also been reported as cross-reactive with feline CD45R/B220.
Compare Similar Items
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Antigen: CD45R (B220)
Applications: Flow Cytometry
Conjugate: Red Fluor 710
Host Species: Rat
Research Discipline: Inflammation, Immunology
Formulation: Purified Rat monoclonal containing 10mM NaH2PO4, 150mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2.
Gene Symbols: Ptprc, Ly-5
Primary or Secondary: Primary
Content And Storage: 2°C to 8°C undiluted for one year from date of shipment protected from light; Do not freeze
Clone: RA3-6B2
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Mouse
Gene Accession No.: P06800
Immunogen: Proprietary immunogen
Purification Method: Purified by size-exclusion chromatography
Antigen:
CD45R (B220)
Applications:
Flow Cytometry
Conjugate:
Red Fluor 710
Host Species:
Rat
Research Discipline:
Inflammation, Immunology
Formulation:
Purified Rat monoclonal containing 10mM NaH2PO4, 150mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2.
Gene Symbols:
Ptprc, Ly-5
Primary or Secondary:
Primary
Content And Storage:
2°C to 8°C undiluted for one year from date of shipment protected from light; Do not freeze
Clone:
RA3-6B2
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Mouse
Gene Accession No.:
P06800
Immunogen:
Proprietary immunogen
Purification Method:
Purified by size-exclusion chromatography
Antigen: CD127 (IL-7Ra)
Applications: Flow Cytometry
Conjugate: Violet Fluor 450 (V450)
Host Species: Rat
Research Discipline: Inflammation, Immunology
Formulation: Purified Rat monoclonal containing 10mM NaH2PO4, 150mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2.
Gene Symbols: Il7r
Primary or Secondary: Primary
Content And Storage: 2°C to 8°C undiluted for one year from date of shipment protected from light; Do not freeze
Clone: A7R34
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Mouse
Gene Accession No.: P16872
Immunogen: Proprietary immunogen
Purification Method: Purified by size-exclusion chromatography
Antigen:
CD127 (IL-7Ra)
Applications:
Flow Cytometry
Conjugate:
Violet Fluor 450 (V450)
Host Species:
Rat
Research Discipline:
Inflammation, Immunology
Formulation:
Purified Rat monoclonal containing 10mM NaH2PO4, 150mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2.
Gene Symbols:
Il7r
Primary or Secondary:
Primary
Content And Storage:
2°C to 8°C undiluted for one year from date of shipment protected from light; Do not freeze
Clone:
A7R34
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Mouse
Gene Accession No.:
P16872
Immunogen:
Proprietary immunogen
Purification Method:
Purified by size-exclusion chromatography
Antigen: CD127 (IL-7Ra)
Applications: Flow Cytometry
Conjugate: Violet Fluor 450 (V450)
Host Species: Rat
Research Discipline: Inflammation, Immunology
Formulation: Purified Rat monoclonal containing 10mM NaH2PO4, 150mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2.
Gene Symbols: Il7r
Primary or Secondary: Primary
Content And Storage: 2°C to 8°C undiluted for one year from date of shipment protected from light; Do not freeze
Clone: A7R34
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Mouse
Gene Accession No.: P16872
Immunogen: Proprietary immunogen
Purification Method: Purified by size-exclusion chromatography
Antigen:
CD127 (IL-7Ra)
Applications:
Flow Cytometry
Conjugate:
Violet Fluor 450 (V450)
Host Species:
Rat
Research Discipline:
Inflammation, Immunology
Formulation:
Purified Rat monoclonal containing 10mM NaH2PO4, 150mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2.
Gene Symbols:
Il7r
Primary or Secondary:
Primary
Content And Storage:
2°C to 8°C undiluted for one year from date of shipment protected from light; Do not freeze
Clone:
A7R34
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Mouse
Gene Accession No.:
P16872
Immunogen:
Proprietary immunogen
Purification Method:
Purified by size-exclusion chromatography
Antigen: CD161 (NK1.1)
Applications: Flow Cytometry
Conjugate: FITC
Host Species: Mouse
Research Discipline: Inflammation, Immunology
Formulation: Purified Mouse monoclonal containing 10mM NaH2PO4, 150mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2.
Gene Symbols: __
Primary or Secondary: Primary
Content And Storage: 2°C to 8°C undiluted for one year from date of shipment protected from light; Do not freeze
Clone: PK136
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Mouse
Gene Accession No.: __
Immunogen: Proprietary immunogen
Purification Method: Purified by size-exclusion chromatography
Antigen:
CD161 (NK1.1)
Applications:
Flow Cytometry
Conjugate:
FITC
Host Species:
Mouse
Research Discipline:
Inflammation, Immunology
Formulation:
Purified Mouse monoclonal containing 10mM NaH2PO4, 150mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2.
Gene Symbols:
__
Primary or Secondary:
Primary
Content And Storage:
2°C to 8°C undiluted for one year from date of shipment protected from light; Do not freeze
Clone:
PK136
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Mouse
Gene Accession No.:
__
Immunogen:
Proprietary immunogen
Purification Method:
Purified by size-exclusion chromatography