Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Phospho-Tyrosine
0.2 mg/mL
Flow Cytometry, Immunohistochemistry (Paraffin), SDS-Page, Immunofluorescence
Unconjugated
Mouse
Human, All species
2 amino 3(4 hydroxyphenyl) propanoic acid, 4 hydroxyphenylalanine, phosphotyrosine, pTyrosine, Tyrosine
IgG2b
Protein A or G purified
Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This MAb shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.
PY793
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 1 - 2 ug/ml, SDS-Page, Immunofluorescence 1 - 2 ug/ml
Monoclonal
Purified
RUO
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Phosphotyrosine conjugated to BSA
Primary
Store at 4C.