S100A9 Antibody (S100A9/1011), Novus Biologicals™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
S100A9
Concentration
0.2 mg/mL
Applications
Flow Cytometry, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunofluorescence
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Cancer, Immunology, Innate Immunity, Signal Transduction, Tyrosine Kinases
Formulation
10mM PBS with 0.05% Sodium Azide
Gene ID (Entrez)
6280
Immunogen
Recombinant human S100A9 protein
Primary or Secondary
Primary
Content And Storage
Store at 4C.
Molecular Weight of Antigen
14 kDa
Clone
S100A9/1011
Dilution
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1 ug/ml, Immunohistochemistry-Frozen (Negative), Immunofluorescence 0.5 - 1.0 ug/ml
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human
Gene Alias
CAGBMigration inhibitory factor-related protein 14, calgranulin B, calgranulin-B, Calprotectin L1H subunit, CFAGMRP-14,60B8AG, CGLB, L1AG, LIAG, MAC387, MIF, MRP14Leukocyte L1 complex heavy chain, NIF, P14, protein S100-A9, S100 calcium binding protein A9, S100 calcium binding protein A9 (calgranulin B), S100 calcium-binding protein A9, S100 calcium-binding protein A9 (calgranulin B)
Gene Symbols
S100A9
Isotype
IgM κ
Purification Method
Protein A or G purified
Test Specificity
Recognizes a 14kDa protein, identified as S100A9 (also known as Calgranulin B or MRP-14); expressed by granulocytes, monocytes and by tissue macrophages.The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Altered expression of this protein is associated with the disease cystic fibrosis. This MAb reacts with neutrophils, monocytes and macrophages, and has been shown as an important marker for identifying macrophages in tissue sections. Among cells that are now recognized as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cel
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Description
- Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, Immunohistochemistry (Frozen) (Negative) S100A9 Monoclonal specifically detects S100A9 in Human samples
- It is validated for Immunohistochemistry, Immunohistochemistry-Paraffin.
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Antigen: S100A9
Concentration: 0.2 mg/mL
Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunofluorescence
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cancer, Immunology, Innate Immunity, Signal Transduction, Tyrosine Kinases
Formulation: 10mM PBS with 0.05% Sodium Azide
Gene ID (Entrez): 6280
Immunogen: Recombinant human S100A9 protein
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Molecular Weight of Antigen: 14 kDa
Clone: S100A9/1011
Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1 ug/ml, Immunohistochemistry-Frozen (Negative), Immunofluorescence 0.5 - 1.0 ug/ml
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: CAGBMigration inhibitory factor-related protein 14, calgranulin B, calgranulin-B, Calprotectin L1H subunit, CFAGMRP-14,60B8AG, CGLB, L1AG, LIAG, MAC387, MIF, MRP14Leukocyte L1 complex heavy chain, NIF, P14, protein S100-A9, S100 calcium binding protein A9, S100 calcium binding protein A9 (calgranulin B), S100 calcium-binding protein A9, S100 calcium-binding protein A9 (calgranulin B)
Gene Symbols: S100A9
Isotype: IgM κ
Purification Method: Protein A or G purified
Test Specificity: Recognizes a 14kDa protein, identified as S100A9 (also known as Calgranulin B or MRP-14); expressed by granulocytes, monocytes and by tissue macrophages.The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. Altered expression of this protein is associated with the disease cystic fibrosis. This MAb reacts with neutrophils, monocytes and macrophages, and has been shown as an important marker for identifying macrophages in tissue sections. Among cells that are now recognized as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells, and Langerhans cells (in normal tissues) and epithelioid cells and Langerhans-type and foreign-body-type multinucleated giant cel
Antigen: S100B
Concentration: 1.0 mg/mL
Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Alzheimers Research, Apoptosis, Biologically Active Proteins, Breast Cancer, Cancer, Cell Biology, Cellular Markers, Epigenetics, Hypoxia, Lipid and Metabolism, Neurodegeneration, Neuronal Cell Markers, Neuroscience, Peroxisome Markers, Signal Transduction, Stem Cells
Formulation: PBS with 0.02% Sodium Azide
Gene ID (Entrez): 6285
Immunogen: Partial recombinant human S100B protein (amino acids 2-92) [Uniprot: P04271].
Primary or Secondary: Primary
Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Molecular Weight of Antigen: __
Clone: 15F9NB
Dilution: Western Blot 2 ug/ml, Flow Cytometry 1:500, Immunohistochemistry 1:100 - 1:500, Immunocytochemistry/Immunofluorescence 1:50-1:200, Immunohistochemistry-Paraffin 1:100 - 1:500
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Alias: beta (neural), NEF, S100, S100 beta, S100 calcium binding protein B, S100 calcium-binding protein B, S100 calcium-binding protein, beta (neural), S-100 calcium-binding protein, beta chain, 10protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100beta
Gene Symbols: S100B
Isotype: IgG2b κ
Purification Method: Protein G purified
Test Specificity: __
Antigen: Semaphorin 4D/CD100
Concentration: 0.2 mg/mL
Applications: Flow Cytometry, Immunofluorescence
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Apoptosis, Immunology
Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene ID (Entrez): 10507
Immunogen: PHA stimulated human peripheral blood lymphocytes.
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Molecular Weight of Antigen: __
Clone: 133-1C6
Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunofluorescence 0.5 - 1.0 ug/ml
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Baboon, Equine (Negative)
Gene Alias: A8, CD100 antigen, CD100M-sema-G, coll-4, FLJ33485, FLJ34282, FLJ39737, FLJ46484, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, 4D, MGC169138, MGC169141, sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4D, semaphorin-4D
Gene Symbols: SEMA4D
Isotype: IgM κ
Purification Method: Protein A or G purified
Test Specificity: Recognizes a homodimeric protein comprised of 50kDa subunits, identified as CD100 (Workshop VI; Code N-L026). It is expressed on majority of haemopoietic cells (B, T, NK and myeloid cells) and is absent from bone marrow, erythrocytes, eosinophils and endothelial cells. Its expression is increased after PHA-activation. CD100 was shown to associate with different partner molecules in T cells such as CD45, a key molecule with protein tyrosine phosphatase activity involved in T-cell transduction, and a Serine kinase. It plays a role in homotypic cell adhesion and in T cell activation.