5545276
CDw60 Antibody (UM4D4), Novus Biologicals™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
CDw60
Concentration
0.2 mg/mL
Applications
Flow Cytometry, Immunohistochemistry (Frozen), Immunofluorescence
Conjugate
Unconjugated
Host Species
Mouse
Target Species
Human
Immunogen
Human rheumatoid synovial T cell line (ST-1)
Primary or Secondary
Primary
Content And Storage
Store at 4C.
Clone
UM4D4
Dilution
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Frozen 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Formulation
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Isotype
IgM
Purification Method
Protein A or G purified
Test Specificity
This MAb reacts with 9-O-acetylated disialosyl group linked to the glycoprotein CD60w. CDw60 is a carbohydrate antigen present on gangliosides, platelets and a subset of CD4+, CD8+peripheral T-cells, but not on B-cells, monocytes or granulocytes. CDw60 is involved in the co-stimulation of T-cells.
Related Products
Description
- Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Frozen), Immunofluorescence CDw60 Monoclonal specifically detects CDw60 in Human samples
- It is validated for ELISA.
Compare Similar Items
Show Difference
Antigen: CDw60
Concentration: 0.2 mg/mL
Applications: Flow Cytometry, Immunohistochemistry (Frozen), Immunofluorescence
Conjugate: Unconjugated
Host Species: Mouse
Target Species: Human
Immunogen: Human rheumatoid synovial T cell line (ST-1)
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Clone: UM4D4
Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Frozen 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Isotype: IgM
Purification Method: Protein A or G purified
Test Specificity: This MAb reacts with 9-O-acetylated disialosyl group linked to the glycoprotein CD60w. CDw60 is a carbohydrate antigen present on gangliosides, platelets and a subset of CD4+, CD8+peripheral T-cells, but not on B-cells, monocytes or granulocytes. CDw60 is involved in the co-stimulation of T-cells.
Antigen:
CDw60
Concentration:
0.2 mg/mL
Applications:
Flow Cytometry, Immunohistochemistry (Frozen), Immunofluorescence
Conjugate:
Unconjugated
Host Species:
Mouse
Target Species:
Human
Immunogen:
Human rheumatoid synovial T cell line (ST-1)
Primary or Secondary:
Primary
Content And Storage:
Store at 4C.
Clone:
UM4D4
Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Frozen 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Isotype:
IgM
Purification Method:
Protein A or G purified
Test Specificity:
This MAb reacts with 9-O-acetylated disialosyl group linked to the glycoprotein CD60w. CDw60 is a carbohydrate antigen present on gangliosides, platelets and a subset of CD4+, CD8+peripheral T-cells, but not on B-cells, monocytes or granulocytes. CDw60 is involved in the co-stimulation of T-cells.
Antigen: CD99
Concentration: 0.2mg/mL
Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
Conjugate: Unconjugated
Host Species: Mouse
Target Species: Human, Rat
Immunogen: Purified E-rosette forming cells from human peripheral blood lymphocytes
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Clone: SPM586
Dilution: Flow Cytometry 5 - 10ul/million cells in 0.1ml, Immunohistochemistry-Paraffin 1 - 2ug/ml, Immunofluorescence 0.5 - 1.0ug/ml, CyTOF-ready
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Formulation: 10mM PBS with 0.05% Sodium Azide
Isotype: IgM κ
Purification Method: Protein A or G purified
Test Specificity: Recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. This antigen is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing s sarcoma cells and primitive peripheral neuroectodermal tumors. This MAb shows a very similar reactivity to other CD99 MAbs (e.g. O13, 12E7, or HBA-71) and is excellent for immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
Antigen:
CD99
Concentration:
0.2mg/mL
Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, CyTOF
Conjugate:
Unconjugated
Host Species:
Mouse
Target Species:
Human, Rat
Immunogen:
Purified E-rosette forming cells from human peripheral blood lymphocytes
Primary or Secondary:
Primary
Content And Storage:
Store at 4C.
Clone:
SPM586
Dilution:
Flow Cytometry 5 - 10ul/million cells in 0.1ml, Immunohistochemistry-Paraffin 1 - 2ug/ml, Immunofluorescence 0.5 - 1.0ug/ml, CyTOF-ready
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Formulation:
10mM PBS with 0.05% Sodium Azide
Isotype:
IgM κ
Purification Method:
Protein A or G purified
Test Specificity:
Recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. This antigen is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing s sarcoma cells and primitive peripheral neuroectodermal tumors. This MAb shows a very similar reactivity to other CD99 MAbs (e.g. O13, 12E7, or HBA-71) and is excellent for immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
Antigen: CD43/Sialophorin
Concentration: 0.2mg/mL
Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Conjugate: Unconjugated
Host Species: Mouse
Target Species: Human
Immunogen: Stimulated human leukocytes
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Clone: 84-3C1
Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 1 - 2 ug/ml
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Isotype: IgG1 κ
Purification Method: Protein A or G purified
Test Specificity: It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43 (Workshop III). 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
Antigen:
CD43/Sialophorin
Concentration:
0.2mg/mL
Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Conjugate:
Unconjugated
Host Species:
Mouse
Target Species:
Human
Immunogen:
Stimulated human leukocytes
Primary or Secondary:
Primary
Content And Storage:
Store at 4C.
Clone:
84-3C1
Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 1 - 2 ug/ml
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Isotype:
IgG1 κ
Purification Method:
Protein A or G purified
Test Specificity:
It recognizes a cell surface glycoprotein of 95/115/135kDa (depending upon the extent of glycosylation), identified as CD43 (Workshop III). 70-90% of T-cell lymphomas and from 22-37% of B-cell lymphomas express CD43. No reactivity has been observed with reactive B-cells. So a B-lineage population that co-expresses CD43 is highly likely to be a malignant lymphoma, especially a low-grade lymphoma, rather than a reactive B-cell population. When CD43 antibody is used in combination with anti-CD20, effective immunophenotyping of the lymphomas in formalin-fixed tissues can be obtained. Co-staining of a lymphoid infiltrate with anti-CD20 and anti-CD43 argues against a reactive process and favors a diagnosis of lymphoma.
Antigen: CA19-9/Sialyl Lewisa
Concentration: 0.2mg/mL
Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Conjugate: Unconjugated
Host Species: Mouse
Target Species: Human
Immunogen: Precipitin lines obtained after immuno-diffusion using MAb 116-NS-19-9 and mucins isolated from an ovarian cyst of a Lewis A+B- patient (0Le).
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Clone: SPM588
Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Isotype: IgM κ
Purification Method: Protein A or G purified
Test Specificity: __
Antigen:
CA19-9/Sialyl Lewisa
Concentration:
0.2mg/mL
Applications:
Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Conjugate:
Unconjugated
Host Species:
Mouse
Target Species:
Human
Immunogen:
Precipitin lines obtained after immuno-diffusion using MAb 116-NS-19-9 and mucins isolated from an ovarian cyst of a Lewis A+B- patient (0Le).
Primary or Secondary:
Primary
Content And Storage:
Store at 4C.
Clone:
SPM588
Dilution:
Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Formulation:
10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Isotype:
IgM κ
Purification Method:
Protein A or G purified
Test Specificity:
__