HLA-A2 Monoclonal Antibody (BB7.2), PE-eFluor™ 610, eBioscience™, Invitrogen™

Description

• Description: This BB7.2 monoclonal antibody recognizes the human major histocompatibility complex (MHC) class I molecule HLA-A2, which is a member of the HLA-A family
• MHC class I exists as a single alpha chain composed of three domains
• This molecule associates with beta 2-microglobulin and is expressed on all nucleated cells
• The BB7.2 monoclonal antibody has been reported to exhibit blocking activity, as well as recognize HLA-A28
• Applications Reported: This BB7.2 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This BB7.2 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
• This may be used at 5 μL (0.25 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• PE-eFluor 610 can be excited with laser lines from 488-561 nm and emits at 607 nm
• We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red)
•  Please make sure that your instrument is capable of detecting this fluorochrome
• Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
• Please protect this vial and stained samples from light
• Fixation: Samples can be stored in IC Fixation Buffer (Product No
• 00-8222-49) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No
• HLA-A belongs to the HLA class I heavy chain paralogues
• This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin)
• The heavy chain is anchored in the membrane
• Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen
• They are expressed in nearly all cells
• The heavy chain is approximately 45 kDa and its gene contains 8 exons
• Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail
• Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule
• Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation
• Hundreds of HLA-A alleles have been described
• HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.