CD28 Monoclonal Antibody (CD28.2), Super Bright™ 645, eBioscience™, Invitrogen™

Description

• Description: The TS2/16 monoclonal antibody reacts with human CD29, also known as integrin beta 1, an approximately 130 kDa single-pass transmembrane glycoprotein
• CD29 complexes with one of nine integrin alpha subunits to form the very late antigen (VLA) subfamily of adhesion molecules
• Integrin heterodimers containing CD29 are involved in cell-cell and cell-matrix adhesion
• CD29 is expressed broadly on lymphocytes and monocytes, with lower levels of expression on granulocytes
• The TS2/16 antibody has been found to possess activating activity for beta 1 integrins
• Applications Reported: This TS2/16 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This TS2/16 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells
• This may be used at 5 μL (0.125 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Super Bright 645 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 645 nm
• We recommend using a 660/20 bandpass filter
• Please make sure that your instrument is capable of detecting this fluorochrome
• When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product No
• CD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein
• CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes
• Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells
• In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death
• In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28
• In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR
• The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells
• Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.