CD40 Monoclonal Antibody (5C3), Super Bright™ 645, eBioscience™, Invitrogen™

Description

• Description: The eBioMWReg30 monoclonal antibody reacts with mouse CD41 (fibrinogen receptor, gpIIb, integrin alpha IIb)
• While initially thought to be expressed exclusively on the surface of platelets and megakaryocytes, it has been demonstrated that CD41 is also expressed on hematopoietic progenitors in the embryo, fetus and adult
• CD41 associates with CD61 (gpIIIa, integrin beta III) to form a receptor which plays a major role in platelet function, including binding of several adhesion molecules such as fibrinogen, fibronectin and vitronectin
• Recently, the SLAM-family markers, CD48 and CD150 have been used to reliably identify hematopoietic stem cells (HSC)
• Specifically, it was found that CD150+CD48- bone marrow cells were highly efficient in their ability to confer long-term multi-lineage reconstitution in irradiated mice
• Furthermore, the efficiency of reconstitution was enhanced when HSCs were further enriched through the exclusion of CD41+ cells
• Thus, the use of CD150+CD48-CD41- as an expression profile efficiently identifies hematopoietic stem cells
• Applications Reported: This eBioMWReg30 (MWReg30) antibody has been reported for use in flow cytometric analysis
• Applications Tested: This eBioMWReg30 (MWReg30) antibody has been tested by flow cytometric analysis of stimulated mouse platelets
• This may be used at less than or equal to 1 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• The CD40 antigen is a single chain glycoprotein that is known to be a member of the tumor necrosis factor/nerve growth factor superfamily and shows a significant homology to the Hodgkin's disease associated antigen, CD30
• CD40 is present on all B cells except plasma cells and is also found on some epithelial cells, carcinomas and lymphoid dendritic cells
• CD40 has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation
• AT-hook transcription factor AKNA is reported to coordinately regulate the expression of CD40, which may be important for homotypic cell interactions
• Adaptor protein TNFR2 interacts with CD40 and serves as a mediator of the signal transduction
• The interaction of CD40 and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis
• Two alternatively spliced transcript variants of CD40 encoding distinct isoforms have been reported
• Diseases associated with CD40 dysfunction include Type 3 Hyper-Igm immunodeficiency and CD40 ligand deficiency.