7068207

Ki-67 Monoclonal Antibody (20Raj1), FITC, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

Ki-67

Applications

Flow Cytometry, Immunocytochemistry, Immunohistochemistry

Conjugate

FITC

Host Species

Mouse

Target Species

Canine, Human

Gene Alias

antigen identified by monoclonal antibody Ki 67; antigen identified by monoclonal antibody Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog; Antigen KI-67; Antigen KI-67 homolog; antigen KI-67; proliferation marker protein Ki-67; antigen KI-67-like; cb31; D630048A14Rik; I79_022666; Ki67; Ki-67; KIA; LOW QUALITY PROTEIN: proliferation marker protein Ki-67; marker of proliferation Ki-67; MIB-; MIB-1; Mki67; PPP1R105; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2; sb:cb31; si:ch211-250b22.7; unnamed protein product; wu:fa11g09; wu:fb57a07; wu:fi14e05

Gene Symbols

Mki67

Primary or Secondary

Primary

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Gene

Mki67

Clone

20Raj1

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Gene Accession No.

P46013

Gene ID (Entrez)

100686578, 4288

Isotype

IgG1 κ

Purification Method

Affinity chromatography

Product Type

Antibody

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Description

  • Description: The monoclonal antibody 20Raj1 recognizes the human Ki-67 protein
  • Two isoforms of Ki-67 exist, a 345 and 395 kDa form that are expressed in dividing cells
  • Ki-67 is expressed in all cell types and is detectable during active phases of the cell cycle (G1, S, G2, and mitosis) but is absent from resting cells (G0)
  • During interphase, Ki-67 expression is localized to the nucleus but redistributes to the chromosomes during mitosis and has specifically been found to associate with heterochromatin-bound proteins such as chromobox protein homolog 3 (CBX3)
  • In studies of tumor cells, Ki-67 expression has been used as a marker for determining the fraction of proliferating cells within a given population of tumor cells
  • This monoclonal antibody 20Raj1 recognizes canine Ki-67
  • Applications Reported: This 20Raj1 antibody has been reported for use in intracellular staining followed by flow cytometric analysis, immunohistochemical staining, and immunocytochemistry
  • Applications Tested: This 20Raj1 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (cat
  • 00-5523) and protocol
  • Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins)
  • This can be used at 5 μL (0.06 μg) per test
  • Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases
  • The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus
  • Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase
  • Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis
  • Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms
  • In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index
  • The correlation between low Ki-67 index and histologically low-grade tumors is strong
  • Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.

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7068207

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Antigen:
Ki-67

Applications:
Flow Cytometry, Immunocytochemistry, Immunohistochemistry

Conjugate:
FITC

Host Species:
Mouse

Target Species:
Canine, Human

Gene Alias:
antigen identified by monoclonal antibody Ki 67; antigen identified by monoclonal antibody Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog; Antigen KI-67; Antigen KI-67 homolog; antigen KI-67; proliferation marker protein Ki-67; antigen KI-67-like; cb31; D630048A14Rik; I79_022666; Ki67; Ki-67; KIA; LOW QUALITY PROTEIN: proliferation marker protein Ki-67; marker of proliferation Ki-67; MIB-; MIB-1; Mki67; PPP1R105; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2; sb:cb31; si:ch211-250b22.7; unnamed protein product; wu:fa11g09; wu:fb57a07; wu:fi14e05

Gene Symbols:
Mki67

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
Mki67

Clone:
20Raj1

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Gene Accession No.:
P46013

Gene ID (Entrez):
100686578, 4288

Isotype:
IgG1 κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

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Gene Symbols:
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Primary or Secondary:
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Gene:
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Clone:
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Form:
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Regulatory Status:
RUO

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Gene Symbols:
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Primary or Secondary:
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Content And Storage:
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Gene:
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Clone:
eBioGL3 (GL-3, GL3), GL3)

Classification:
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Form:
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110066, 110067

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Isotype:
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