IL-5 Monoclonal Antibody (TRFK5), PE, eBioscience™, Invitrogen™

Description

• Description: The TRFK5 antibody reacts with mouse and human interleukin-5 (IL-5)
• The TRFK5 antibody is a neutralizing antibody
• Mouse IL-5 is a disulfide-linked homodimer, containing two 113 amino acid peptides; these resolve as 32-34 kDa bands in SDS-PAGE
• IL-5 is produced by T cells and has been known as eosinophil-differentiating factor (EDF), B cell growth factor II (BCGFII), and T cell-replacing factor (TRF)
• IL-5 induces eosinophil differentiation and promotes eosinophil survival and activation
• In mice, IL-5 has been shown to stimulate B cell proliferation and antibody production
• Applications Reported: This TRFK5 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
• Applications Tested: This TRFK5 antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (cat
• 88-8824) and protocol
• Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online
• This can be used at less than or equal to 0.25 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• IL-5 is a cytokine that acts as a growth and differentiation factor for both B cells and eosinophils
• IL-5 cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation
• The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes
• The receptor of IL-5 cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (IL3) and colony stimulating factor 2 (CSF2/GM-CSF)
• IL-5, together with those for interleukin 4 (IL4), interleukin 13 (IL13), and CSF2, form a cytokine gene cluster on chromosome 5
• IL-5, IL4, and IL13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31.