ZO-1 Monoclonal Antibody (R26.4C), eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody R26.4C recognizes the tight junction protein 1, ZO-1 (zona occludens) of rat, mouse, canine, and porcine
• ZO-1 is a member of the MAGUK family of tight junction associated scaffolding proteins, each containing a PDZ, SH3 and guanylate kinase-like (GUK) domain
• ZO-1 is an integral member of the tight junction complex which forms a barrier to paracellular movement of substances, separating apical and basolateral fluids in relation to the epithelial cell layer
• ZO-1 forms complexes with family members ZO-2, ZO-3, as well as occludin, cingulin, claudin-1-8, actin, alpha-catenin, the Ras-effector molecule AF-6, ZONAB, Apg-2, and the junctional adhesion molecules (JAM)-A-C
• ZO-1 is thought to anchor the actin cytoskeleton to the tight junction
• ZO-1 and ZO-2 have been shown to regulate cell cycle and proliferation with recent evidence highlighting the down-regulation of ZO-1 and ZO-2 with cancer progression
• Applications Reported: This R26.4C antibody has been reported for use in western blotting, immunohistochemical staining of frozen tissue sections, microscopy, and immunocytochemistry
• Applications Tested: This R26.4C antibody has been tested by immunocytochemistry of formaldehyde-fixed and permeabilized cells and by immunohistochemistry of frozen mouse tissue and can be used at less than or equal to 10 μg/mL
• It is recommended that the antibody be carefully titrated for optimal performance in the assay interest
• ZO-1 forms complexes with either ZO-2 or ZO-3
• In addition, these proteins can also associate with claudin, occludin and F-actin, at tight junction stands, where they provide a linkage between the actin cytoskeleton and the tight junction
• ZO-1 expression is significantly reduced in many breast cancer lines
• ZO-2 and ZO-3 are ubiquitously expressed within epithelial tight junctions, and unlike ZO-1, which is also expressed at cell junctions of cardiac myocytes, ZO-2 is not expressed in nonepithelial tissue
• ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions
• ZO-1 may be involved in signal transduction at cell-cell junctions
• Two transcript variants encoding distinct isoforms have been identified for the ZO-1 gene
• Diseases associated with ZO-1 dysfunction include Celiac Disease and Congenital Nephrotic Syndrome.