Phospho-S6 (Ser235, Ser236) Monoclonal Antibody (cupk43k), APC, eBioscience™, Invitrogen™

Description

• Description: This cupk43k monoclonal antibody recognizes human and mouse ribosomal protein S6 (also known as 40S ribosomal protein S6, phosphoprotein NP33, RPS6, RS6, S6) when phosphorylated on serine 235 (S235, human) and serine 236 (S236, mouse)
• Ribosomal protein S6 is a component of the 40S subunit of the ribosome and is phosphorylated at multiple sites following stimulation of cells by growth factors, tumor promoting agents, or mitogens
• Phosphorylation of ribosomal protein S6 by p70S6K and PKDCD results in upregulation of the translation of RNA coding for proteins involved in cell cycle entry
• Ribosomal protein S6 is dephosphorylated upon growth arrest
• The specificity of the cupk43k monoclonal antibody was determined by western blotting
• Applications Reported: This cupk43k antibody has been reported for use in intracellular staining followed by flow cytometric analysis
• Applications Tested: This cupk43k antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated normal human peripheral blood cells
• This can be used at 5 μL (0.06 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Phospho-S6 (Ser235, Ser236) Monoclonal antibody specifically detects Phospho-S6 (Ser235, Ser236) in Human, Mouse samples
• It is validated for Flow Cytometry.