Phospho-STAT4 (Tyr693) Monoclonal Antibody (4LURPIE), APC, eBioscience™, Invitrogen™

Description

• Description: This 4LURPIE monoclonal antibody recognizes human and mouse signal transducer and activator of transcription 4 (STAT4) when phosphorylated on tyrosine 693 (Y693)
• STAT proteins are activated by ligand binding to cytokine receptors that associate with Janus kinase (JAK) family members
• Following their phosphorylation by JAK proteins, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine-specific manner
• STAT4 is activated by IL-12 or type 1 IFN (IFN alpha, IFN beta)
• STAT4 is required for optimal Th1 differentiation in vivo
• Interestingly, although IFN alpha can lead to phosphorylation of STAT4 in mouse T cells, the phosphorylation is much weaker than that induced by IL-12 and is transient
• Thus, IFN alpha phosphorylation of STAT4 does not contribute to STAT4-mediated Th1 development or IFN gamma production by Th1 cells in mice
• In contrast, IFN alpha does promote Th1 development and function of human CD4+ T cells
• Specificity of this 4LURPIE clone was determined by ELISA and flow cytometry
• Applications Reported: This 4LURPIE antibody has been reported for use in intracellular staining followed by flow cytometric analysis
• Applications Tested: This 4LURPIE antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells
• This can be used at 5 μL (0.25 μg) per test
• Phospho-STAT4 (Tyr693) Monoclonal antibody specifically detects Phospho-STAT4 (Tyr693) in Human, Mouse samples
• It is validated for Flow Cytometry.