Phospho-STAT6 (Tyr641) Monoclonal Antibody (CHI2S4N), APC, eBioscience™, Invitrogen™

Description

• Description: This CHI2S4N monoclonal antibody recognizes human and mouse signal transducer and activator of transcription 6 (STAT6) when phosphorylated on tyrosine 641
• Following their phosphorylation by JAKs, STAT proteins translocate to the nucleus where they bind to DNA and regulate transcription of specific genes in a cell type- and cytokine-specific manner
• In response to IL-4, STAT6 is phosphorylated on tyrosine 641 by JAK1 and JAK3
• STAT6 signaling downstream of the IL-4 receptor promotes T cell growth and B cell production of IgE
• Specificity of this CHI2S4N clone was determined by ELISA and flow cytometry
• Applications Reported: This CHI2S4N antibody has been reported for use in intracellular staining followed by flow cytometric analysis
• Applications Tested: This CHI2S4N antibody has been pre-titrated and tested by intracellular staining followed by flow cytometric analysis of stimulated normal human peripheral blood cells
• This can be used at 5 μL (0.06 μg) per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• Staining Protocol: We recommend using Protocol C: Two-step protocol: Fixation/Methanol
• Protocol A: Two-step protocol: intracellular (cytoplasmic) proteins and Protocol B: One-step protocol: intracellular (nuclear) proteins cannot be used
• STAT6 protein is a transcription factor activated by cytokines, particularly interleukin-4 and IL13
• (Stat6-/-) were found to be deficient in IL-4-mediated functions including Th2 helper T-cell differentiation, expression of cell surface markers, T-cell proliferation, immunoglobulin class switching to IgE, and partial loss of IL-4 mediated proliferation
• STAT6 mRNA has been detected in peripheral blood lymphocytes, colon, intestine, ovary, prostate, thymus, spleen, kidney, liver, lung and placenta
• STAT6 is critically involved in Th2 immune response
• STAT6 is crucial for IL-4-mediated biological responses, and naturally in two isoforms: STAT6a and STAT6c.