FR4 Monoclonal Antibody (eBio12A5), PE-Cyanine7, eBioscience™, Invitrogen™

Description

• Description: The monoclonal antibody eBio12A5 recognizes FR4, also known as Folate receptor 4, FR delta and FBP3 (folate binding protein3)
• FR4 is a heavily glycosylated, 35 kD receptor for folic acid and the physiologic circulating form of the vitamin, N5-methyltetrahydrofolate
• Natural Treg have high levels of FR4 and together with CD25 can be used to distinguish natural Treg, effector T cells, memory-like T cells and naive T cells
• FR4 high CD25 high cells are natural Treg with high levels of Foxp3
• FR4 high CD25 low cells are identified as central memory T cells, which upon stimulation, can proliferate and produce large amounts of IL-2
• In contrast, FR4 low CD25 high cells secrete proinflammatory cytokines such as IFN gamma and IL-17 and have been characterized as effector memory T cells
• Based on co-staining studies, the epitopes recognized by eBioTH6 and eBio12A5 are different, thereby allowing functional studies with eBioTH6 to be evaluated with eBio12A5
• Applications Reported: This eBio12A5 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This eBio12A5 antibody has been tested by flow cytyometric analysis of mouse splenocytes
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• FR4 is a heavily glycosylated 35 kD receptor for folic acid and the physiologic circulating form of the vitamin, N5-methyltetrahydrofolate
• Natural T regs have high levels of FR4 and together with CD25 can be used to distinguish natural Tregs, effector T cells, memory-like T cells and naive T cells
• In vivo studies with eBioTH6 show a decrease in the number of CD4+CD25+ cells and an enhanced tumor response leading to the elimination of advanced stages of tumors.