7090745
CD80 (B7-1) Monoclonal Antibody (2D10.4), PerCP-eFluor™ 710, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
CD80 (B7-1)
Concentration
5 μL/Test
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias
Activation B7-1 antigen; B7; B7 protein; B7.1; B71; B7-1; B7-1 protein; B7-1 protein precursor; BB1; B-lymphocyte activation antigen B7; Cd28l; CD28LG; CD28LG1; CD80; Cd80 a rat homolog of the human CD28/CTLA - 4 ligand (B7-1); CD80 anitgen; CD80 antigen; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); Cd80 molecule; CD80 protein precursor; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; LAB7; Ly53; Ly-53; MIC17; sCD 80; sCD80; secreted B7-1 protein precursor; soluble CD 80; soluble CD80; T-cell costimulatory molecule; T-cell co-stimulatory protein B7-1; T-lymphocyte activation antigen CD80; TS/A-1; TSA1
Gene Symbols
CD80
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
CD80
Clone
2D10.4
Applications
Flow Cytometry
Conjugate
PerCP-eFluor 710
Host Species
Mouse
Target Species
Human
Gene Accession No.
P33681
Gene ID (Entrez)
941
Isotype
IgG1 κ
Purification Method
Affinity chromatography
Product Type
Antibody
Related Products
Description
- Description: The 2D10.4 monoclonal antibody reacts with human CD80 (B7-1), a 60 kDa member of the Ig superfamily
- CD80 is expressed by activated B cells, macrophages and dendritic cells
- In addition, activated T cells express this antigen
- CD80 has high affinity for binding to two T cell surface antigens, CD28 and CD152 (CTLA-4)
- The interaction of CD28 and CD152 with CD80 is crucial in T-B cell communication leading to activation of T and B cells, respectively
- Applications Reported: This 2D10.4 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This 2D10.4 antibody has been pre-titrated and tested by flow cytometric analysis of Daudi cell line
- This can be used at 5 μL (0.5 μg) per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5
- We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative
- Please make sure that your instrument is capable of detecting this fluorochrome
- Fixation: Samples can be stored in IC Fixation Buffer (cat
- 00-8222) (100 μL cell sample + 100 μL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat
- CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152)
- Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process
- CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes
- The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells
- Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.
Compare Similar Items
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Antigen: CD80 (B7-1)
Concentration: 5 μL/Test
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias: Activation B7-1 antigen; B7; B7 protein; B7.1; B71; B7-1; B7-1 protein; B7-1 protein precursor; BB1; B-lymphocyte activation antigen B7; Cd28l; CD28LG; CD28LG1; CD80; Cd80 a rat homolog of the human CD28/CTLA - 4 ligand (B7-1); CD80 anitgen; CD80 antigen; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); Cd80 molecule; CD80 protein precursor; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; LAB7; Ly53; Ly-53; MIC17; sCD 80; sCD80; secreted B7-1 protein precursor; soluble CD 80; soluble CD80; T-cell costimulatory molecule; T-cell co-stimulatory protein B7-1; T-lymphocyte activation antigen CD80; TS/A-1; TSA1
Gene Symbols: CD80
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: CD80
Clone: 2D10.4
Applications: Flow Cytometry
Conjugate: PerCP-eFluor 710
Host Species: Mouse
Target Species: Human
Gene Accession No.: P33681
Gene ID (Entrez): 941
Isotype: IgG1 κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
CD80 (B7-1)
Concentration:
5 μL/Test
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias:
Activation B7-1 antigen; B7; B7 protein; B7.1; B71; B7-1; B7-1 protein; B7-1 protein precursor; BB1; B-lymphocyte activation antigen B7; Cd28l; CD28LG; CD28LG1; CD80; Cd80 a rat homolog of the human CD28/CTLA - 4 ligand (B7-1); CD80 anitgen; CD80 antigen; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); Cd80 molecule; CD80 protein precursor; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; LAB7; Ly53; Ly-53; MIC17; sCD 80; sCD80; secreted B7-1 protein precursor; soluble CD 80; soluble CD80; T-cell costimulatory molecule; T-cell co-stimulatory protein B7-1; T-lymphocyte activation antigen CD80; TS/A-1; TSA1
Gene Symbols:
CD80
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
CD80
Clone:
2D10.4
Applications:
Flow Cytometry
Conjugate:
PerCP-eFluor 710
Host Species:
Mouse
Target Species:
Human
Gene Accession No.:
P33681
Gene ID (Entrez):
941
Isotype:
IgG1 κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: CD90 (Thy-1)
Concentration: 5 μL/Test
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias: CD7; CD90; CDw90; FLJ33325; membrane glycoprotein; T25; Thy 1.2; THY1; Thy-1; Thy-1 antigen; Thy-1 cell surface antigen; thy-1 glycoprotein; thy-1 membrane glycoprotein; Thy-1 protein; Thy-1 T-cell antigen; Thy1.1; Thy1.2; Thy-1.2; thymus cell antigen 1, theta; Thymus cell surface antigen
Gene Symbols: Thy1
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: Thy1
Clone: eBio5E10 (5E10)
Applications: Flow Cytometry
Conjugate: PerCP-eFluor 710
Host Species: Mouse
Target Species: Human
Gene Accession No.: P04216
Gene ID (Entrez): 7070
Isotype: IgG1 κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
CD90 (Thy-1)
Concentration:
5 μL/Test
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias:
CD7; CD90; CDw90; FLJ33325; membrane glycoprotein; T25; Thy 1.2; THY1; Thy-1; Thy-1 antigen; Thy-1 cell surface antigen; thy-1 glycoprotein; thy-1 membrane glycoprotein; Thy-1 protein; Thy-1 T-cell antigen; Thy1.1; Thy1.2; Thy-1.2; thymus cell antigen 1, theta; Thymus cell surface antigen
Gene Symbols:
Thy1
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
Thy1
Clone:
eBio5E10 (5E10)
Applications:
Flow Cytometry
Conjugate:
PerCP-eFluor 710
Host Species:
Mouse
Target Species:
Human
Gene Accession No.:
P04216
Gene ID (Entrez):
7070
Isotype:
IgG1 κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: CD103 (Integrin alpha E)
Concentration: 0.2 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: A530055J10; alpha-E1; alpha-M290; aM290; antigen CD103; antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide; CD103; DC; DCs; HML-1 antigen; human mucosal lymphocyte antigen 1, alpha polypeptide; HUMINAE; integrin alpha E; integrin alpha E, epithelial-associated; integrin alpha E1, epithelial-associated; integrin alpha M290; Integrin alpha-E; Integrin alpha-E heavy chain; Integrin alpha-E light chain; Integrin alpha-IEL; integrin subunit alpha E; integrin, alpha E; integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide); integrin, alpha E, epithelial-associated; Intregrin alpha E2; Itgae; MGC141996; Mucosal lymphocyte 1 antigen
Gene Symbols: Itgae
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: Itgae
Clone: 2E7
Applications: Flow Cytometry
Conjugate: PerCP-eFluor 710
Host Species: Armenian Hamster
Target Species: Mouse
Gene Accession No.: Q60677
Gene ID (Entrez): 16407
Isotype: IgG
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
CD103 (Integrin alpha E)
Concentration:
0.2 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
A530055J10; alpha-E1; alpha-M290; aM290; antigen CD103; antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide; CD103; DC; DCs; HML-1 antigen; human mucosal lymphocyte antigen 1, alpha polypeptide; HUMINAE; integrin alpha E; integrin alpha E, epithelial-associated; integrin alpha E1, epithelial-associated; integrin alpha M290; Integrin alpha-E; Integrin alpha-E heavy chain; Integrin alpha-E light chain; Integrin alpha-IEL; integrin subunit alpha E; integrin, alpha E; integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide); integrin, alpha E, epithelial-associated; Intregrin alpha E2; Itgae; MGC141996; Mucosal lymphocyte 1 antigen
Gene Symbols:
Itgae
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
Itgae
Clone:
2E7
Applications:
Flow Cytometry
Conjugate:
PerCP-eFluor 710
Host Species:
Armenian Hamster
Target Species:
Mouse
Gene Accession No.:
Q60677
Gene ID (Entrez):
16407
Isotype:
IgG
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: CD103 (Integrin alpha E)
Concentration: 5 μL/Test
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias: A530055J10; alpha-E1; alpha-M290; aM290; antigen CD103; antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide; CD103; DC; DCs; HML-1 antigen; human mucosal lymphocyte antigen 1, alpha polypeptide; HUMINAE; integrin alpha E; integrin alpha E, epithelial-associated; integrin alpha E1, epithelial-associated; integrin alpha M290; Integrin alpha-E; Integrin alpha-E heavy chain; Integrin alpha-E light chain; Integrin alpha-IEL; integrin subunit alpha E; integrin, alpha E; integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide); integrin, alpha E, epithelial-associated; Intregrin alpha E2; Itgae; MGC141996; Mucosal lymphocyte 1 antigen
Gene Symbols: Itgae
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: Itgae
Clone: Ber-ACT8
Applications: Flow Cytometry
Conjugate: PerCP-eFluor 710
Host Species: Mouse
Target Species: Human
Gene Accession No.: P38570
Gene ID (Entrez): 3682
Isotype: IgG1 κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
CD103 (Integrin alpha E)
Concentration:
5 μL/Test
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias:
A530055J10; alpha-E1; alpha-M290; aM290; antigen CD103; antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide; CD103; DC; DCs; HML-1 antigen; human mucosal lymphocyte antigen 1, alpha polypeptide; HUMINAE; integrin alpha E; integrin alpha E, epithelial-associated; integrin alpha E1, epithelial-associated; integrin alpha M290; Integrin alpha-E; Integrin alpha-E heavy chain; Integrin alpha-E light chain; Integrin alpha-IEL; integrin subunit alpha E; integrin, alpha E; integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide); integrin, alpha E, epithelial-associated; Intregrin alpha E2; Itgae; MGC141996; Mucosal lymphocyte 1 antigen
Gene Symbols:
Itgae
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
Itgae
Clone:
Ber-ACT8
Applications:
Flow Cytometry
Conjugate:
PerCP-eFluor 710
Host Species:
Mouse
Target Species:
Human
Gene Accession No.:
P38570
Gene ID (Entrez):
3682
Isotype:
IgG1 κ
Purification Method:
Affinity chromatography
Product Type:
Antibody