CD45 Monoclonal Antibody (30-F11), Alexa Fluor™ 700, eBioscience™, Invitrogen™

Description

• Description: The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45, also known as Leukocyte Common Antigen (LCA)
• CD45 is expressed by all hematopoietic cells excluding mature erythrocytes and platelets
• The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes
• Applications Reported: This 30-F11 antibody has been reported for use in flow cytometric analysis
• Applications Tested: This 30-F11 antibody has been tested by flow cytometric analysis of mouse splenocytes
• This can be used at less than or equal to 0.5 μg per test
• A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
• Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
• It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
• Alexa Fluor™ 700 emits at 723 nm and can be excited with the red laser (633 nm)
• Most instruments will require a 685 LP mirror and 710/20 filter
• Please make sure that your instrument is capable of detecting this fluorochrome
• Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser
• Filtration: 0.2 μm post-manufacturing filtered
• CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes
• CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues
• CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases
• CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains
• CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells
• CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell
• CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling
• CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.