7091728

CD45 Monoclonal Antibody (30-F11), eFluor™ 506, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

CD45

Concentration

0.2 mg/mL

Classification

Monoclonal

Form

Liquid

Regulatory Status

RUO

Formulation

PBS with 0.09% sodium azide; pH 7.2

Gene Alias

B220; cd45; CD45 antigen; CD45 antigen isoform 1 precursor; CD45 antigen isoform 2 precursor; CD45 antigen isoform 3 precursor; CD45 antigen isoform 4 precursor; CD45 antigen isoform 5 precursor; CD45 antigen isoform 6 precursor; CD45R; GP180; Lca; L-CA; leucocyte common antigen; leukocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; leukocyte common antigen, CD45; loc; LOW QUALITY PROTEIN: receptor-type tyrosine-protein phosphatase C; LY5; Ly-5; lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; membrane tyrosine phosphatase; protein tyrosine phosphatase lambda; protein tyrosine phosphatase receptor type C; protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Protein tyrosine phosphatase, receptor-type, c polypeptide; protein tyrosine phosphatase; alternatively spliced; Ptprc; Receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220

Gene Symbols

PTPRC

Primary or Secondary

Primary

Content And Storage

4° C, store in dark, DO NOT FREEZE!

Gene

PTPRC

Clone

30-F11

Applications

Flow Cytometry

Conjugate

eFluor 506

Host Species

Rat

Target Species

Mouse

Gene Accession No.

P06800

Gene ID (Entrez)

19264

Isotype

IgG2b κ

Purification Method

Affinity chromatography

Product Type

Antibody

Related Products

Img

Fischer Scientific

7091536

Rat Monoclonal Antibody...

Img

Fischer Scientific

7091564

Mouse Monoclonal Antibody...

Img

Fischer Scientific

7092258

Mouse Monoclonal Antibody...

Img

Fischer Scientific

7092257

Rat Monoclonal Antibody...

Img

Fischer Scientific

7091538

Mouse Monoclonal Antibody...

Img

Fischer Scientific

7105446

Mouse Monoclonal Antibody...

Img

Fischer Scientific

7092287

Rat Monoclonal Antibody...

Img

Fischer Scientific

7091535

Rat Monoclonal Antibody...

Description

  • Description: The 30-F11 monoclonal antibody reacts with all isoforms of mouse CD45, also known as Leukocyte Common Antigen (LCA)
  • CD45 is expressed by all hematopoietic cells excluding mature erythrocytes and platelets
  • The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes
  • Applications Reported: This 30-F11 antibody has been reported for use in flow cytometric analysis
  • Applications Tested: This 30-F11 antibody has been tested by flow cytometric analysis of mouse bone marrow cells
  • This can be used at less than or equal to 0.5 μg per test
  • A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
  • Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
  • It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
  • eFluor™ 506 can be excited with the violet laser line (405 nm) and emits at 506 nm
  • We recommend using a 510/20 band pass filter, or equivalent
  • Please make sure that your instrument is capable of detecting this fluorochrome
  • Excitation: 405 nm; Emission: 506 nm; Laser: Violet Laser
  • Filtration: 0.2 μm post-manufacturing filtered
  • CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes
  • CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues
  • CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases
  • CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains
  • CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells
  • CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell
  • CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling
  • CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

Compare Similar Items

Show Difference

Img

Fischer Scientific

7091728

Rat Monoclonal Antibody...


Antigen:
CD45

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
B220; cd45; CD45 antigen; CD45 antigen isoform 1 precursor; CD45 antigen isoform 2 precursor; CD45 antigen isoform 3 precursor; CD45 antigen isoform 4 precursor; CD45 antigen isoform 5 precursor; CD45 antigen isoform 6 precursor; CD45R; GP180; Lca; L-CA; leucocyte common antigen; leukocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; leukocyte common antigen, CD45; loc; LOW QUALITY PROTEIN: receptor-type tyrosine-protein phosphatase C; LY5; Ly-5; lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; membrane tyrosine phosphatase; protein tyrosine phosphatase lambda; protein tyrosine phosphatase receptor type C; protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Protein tyrosine phosphatase, receptor-type, c polypeptide; protein tyrosine phosphatase; alternatively spliced; Ptprc; Receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220

Gene Symbols:
PTPRC

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
PTPRC

Clone:
30-F11

Applications:
Flow Cytometry

Conjugate:
eFluor 506

Host Species:
Rat

Target Species:
Mouse

Gene Accession No.:
P06800

Gene ID (Entrez):
19264

Isotype:
IgG2b κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

Img

Fischer Scientific

7091751

Mouse Monoclonal Antibody...


Antigen:
FOXP3

Concentration:
5 μL/Test

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Alias:
AIID; DIETER; forkhead box P3; forkhead box protein P3; Forkhead box protein P3 41 kDa form; Forkhead box protein P3, C-terminally processed; forkhead/winged helix transcription factor 3; Foxp3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; IPEX; JM2; MGC141961; MGC141963; PIDX; regulatory protein Foxp3; RGD1562112; RP23-54C14.1; scurfin; scurfy; sf; XPID

Gene Symbols:
Foxp3

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
Foxp3

Clone:
150D/E4

Applications:
Flow Cytometry

Conjugate:
PE

Host Species:
Mouse

Target Species:
Human, Mouse, Rat

Gene Accession No.:
Q99JB6, Q9BZS1

Gene ID (Entrez):
20371, 317382, 50943

Isotype:
IgG1 κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

Img

Fischer Scientific

7091751

Mouse Monoclonal Antibody...


Antigen:
FOXP3

Concentration:
5 μL/Test

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2

Gene Alias:
AIID; DIETER; forkhead box P3; forkhead box protein P3; Forkhead box protein P3 41 kDa form; Forkhead box protein P3, C-terminally processed; forkhead/winged helix transcription factor 3; Foxp3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; IPEX; JM2; MGC141961; MGC141963; PIDX; regulatory protein Foxp3; RGD1562112; RP23-54C14.1; scurfin; scurfy; sf; XPID

Gene Symbols:
Foxp3

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
Foxp3

Clone:
150D/E4

Applications:
Flow Cytometry

Conjugate:
PE

Host Species:
Mouse

Target Species:
Human, Mouse, Rat

Gene Accession No.:
Q99JB6, Q9BZS1

Gene ID (Entrez):
20371, 317382, 50943

Isotype:
IgG1 κ

Purification Method:
Affinity chromatography

Product Type:
Antibody

Img

Fischer Scientific

7091756

Armenian Hamster Monoclonal Antibod...


Antigen:
CD80 (B7-1)

Concentration:
0.2 mg/mL

Classification:
Monoclonal

Form:
Liquid

Regulatory Status:
RUO

Formulation:
PBS with 0.09% sodium azide; pH 7.2

Gene Alias:
Activation B7-1 antigen; B7; B7 protein; B7.1; B71; B7-1; B7-1 protein; B7-1 protein precursor; BB1; B-lymphocyte activation antigen B7; Cd28l; CD28LG; CD28LG1; CD80; Cd80 a rat homolog of the human CD28/CTLA - 4 ligand (B7-1); CD80 anitgen; CD80 antigen; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); Cd80 molecule; CD80 protein precursor; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CTLA-4 counter-receptor B7.1; LAB7; Ly53; Ly-53; MIC17; sCD 80; sCD80; secreted B7-1 protein precursor; soluble CD 80; soluble CD80; T-cell costimulatory molecule; T-cell co-stimulatory protein B7-1; T-lymphocyte activation antigen CD80; TS/A-1; TSA1

Gene Symbols:
CD80

Primary or Secondary:
Primary

Content And Storage:
4° C, store in dark, DO NOT FREEZE!

Gene:
CD80

Clone:
16-10A1

Applications:
Flow Cytometry

Conjugate:
PerCP-eFluor 710

Host Species:
Armenian Hamster

Target Species:
Canine, Mouse, Porcine

Gene Accession No.:
Q00609

Gene ID (Entrez):
12519, 397161, 403765

Isotype:
IgG

Purification Method:
Affinity chromatography

Product Type:
Antibody