ROR gamma (t) Monoclonal Antibody (B2D), PE-eFluor™ 610, eBioscience™, Invitrogen™

Description

• Description: The B2D monoclonal antibody reacts with the mouse ROR gamma (t) protein
• ROR gamma is a member of the retinoic acid-related orphan receptor (ROR) family, which also includes ROR alpha and ROR beta
• ROR family proteins are ligand-dependent transcription factors that play roles in multiple physiological processes
• ROR gamma is expressed in several tissues including liver, lung, muscle, heart and kidney
• Furthermore, it was discovered that alternative transcription results in the expression of an isoform, ROR gamma t, which is expressed exclusively in cells of the lymphoid compartment, namely CD4+CD8+ double-positive thymoyctes, Th17 cells of the periphery and lymphoid tissue inducer (Lti) cells of lymphoid organs
• The ROR gamma t isoform differs from ROR gamma by three unique amino acids at the amino terminus
• Therefore, the B2D antibody will react with both the ROR gamma and ROR gamma t isoforms
• Applications Reported: This B2D antibody has been reported for use in intracellular staining followed by flow cytometric analysis
• Applications Tested: This B2D antibody has been tested by intracellular staining and flow cytometric analysis of mouse splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (cat
• 00-5523) and protocol
• Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular protein under the Resources Tab online
• This can be used at less than or equal to 0.25 μg per test
• ROR gamma, a NR1 Thyroid Hormone-Like receptor, has been shown to affect thymopoiesis, bone metabolism, T-cell apoptosis, and lymphoid organogenesis
• ROR gamma has been shown to promote thymocyte survival by activating the expression of the antiapoptotic protein Bcl-x(L)
• ROR gamma is also required for the development of lymph nodes and Peyer's patches
• It has been shown that ROR gamma(t), a thymus-specific isoform of ROR gamma from mouse, inhibits Fas ligand expression and cytokine secretion in immature thymocytes
• ROR gamma binds as a monomer to response elements composed of a single core motif, GGTCA, preceded by a 6 bp AT-rich sequence
• ROR gamma expression has been documented in mouse thymus, adipose, bone, skeletal muscle, liver, and kidney, and in human skeletal muscle
• ESTs have been isolated from human tissue libraries, including cancerous head/neck, germ cell, and prostate, and normal colon, head/neck, lung, liver/spleen, and pancreas
• ROR gammat t (RORgt) is an isoform of RORC.