7091645
IFN gamma Monoclonal Antibody (XMG1.2), PE-eFluor™ 610, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
IFN gamma
Concentration
0.2 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Alias
BoIFNG; cytokine; EGK_03901; gamma interferon; gamma interferon precursor; gamma-interferon; gamma-interferon precursor (AA -23 to 143); H-IFN-g; IFG; IFI; IFN gamma; IFN ?; Ifng; IFN-g; IFNG2; IFN-gamma; IFN-gamma precursor; IFN-y; IFN?; Immune interferon; interferon gamma; interferon gamma precursor; interferon gamma type 2; Interferon y; Interferon ?; interferon, gamma; interferon-gama; interferon-gamma; Interferon-gamma level; interferon-gamma precursor; Interferon?; M-IFN-g; R-IFN-g
Gene Symbols
IFNG
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
IFNG
Clone
XMG1.2
Applications
Flow Cytometry
Conjugate
PE-eFluor 610
Host Species
Rat
Target Species
Mouse
Gene Accession No.
P01580
Gene ID (Entrez)
15978
Isotype
IgG1 κ
Purification Method
Affinity chromatography
Product Type
Antibody
Related Products
Description
- Description: The XMG1.2 antibody reacts with mouse interferon (IFN) gamma
- The XMG1.2 antibody is a neutralizing antibody
- Mouse IFN gamma is a 20 kDa factor produced by activated T, B and NK cells, and is an anti-viral and anti-parasitic cytokine
- IFN gamma, in synergy with other cytokines such as TNF alpha, inhibits proliferation of normal and transformed cells
- Immunomodulatory effects of IFN gamma are exerted on a wide range of cell types expressing the high affinity receptors for IFN gamma
- Glycosylation of IFN gamma does not affect its biological activity
- Applications Reported: This XMG1.2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis
- Applications Tested: This XMG1.2 antibody has been tested by intracellular staining and flow cytometric analysis of stimulated mouse splenocytes using the Intracellular Fixation & Permeabilization Buffer Set (cat
- 88-8824) and protocol
- Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online
- This can be used at less than or equal to 0.25 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor, and immune interferon that is produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, Staphylococcus enterotoxin B, phytohemaglutanin and other cytokines
- IFN gamma is a dimeric protein consisting of two 146 amino acid subunits
- IFN gamma is a glycoprotein that exists, functionally, as a homodimer of approximately 45 kDa
- On SDS-PAGE, IFN gamma appears as a combination of 25, 20 and minor 15.5 kDa bands as a result of differential glycosylation
- The biological activity of the IFN gamma homodimer is highly species specific
- Human IFN gamma does not show cross-reactivity with mouse
- IFN gamma function includes the following: antiviral activity, tumor antiproliferative activity, induction of class I and II MHC, macrophage activation, and enhanced immunoglobulin secretion by B lymphocytes
- IFN gamma is involved in cytokine regulation and also acts synergistically with other cytokines
- Activation of IFN gamma takes place through binding of IFN gamma receptor I and II, and activating the JAK-STAT pathway
- IFN gamma does not show any homology with IFN alpha or IFN beta but human IFN gamma shows about 40% sequence homology with mouse IFN gamma
- IFN gamma is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN
- IFN gamma gene mutations are associated with aplastic anemia.
Compare Similar Items
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Antigen: IFN gamma
Concentration: 0.2 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: BoIFNG; cytokine; EGK_03901; gamma interferon; gamma interferon precursor; gamma-interferon; gamma-interferon precursor (AA -23 to 143); H-IFN-g; IFG; IFI; IFN gamma; IFN ?; Ifng; IFN-g; IFNG2; IFN-gamma; IFN-gamma precursor; IFN-y; IFN?; Immune interferon; interferon gamma; interferon gamma precursor; interferon gamma type 2; Interferon y; Interferon ?; interferon, gamma; interferon-gama; interferon-gamma; Interferon-gamma level; interferon-gamma precursor; Interferon?; M-IFN-g; R-IFN-g
Gene Symbols: IFNG
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: IFNG
Clone: XMG1.2
Applications: Flow Cytometry
Conjugate: PE-eFluor 610
Host Species: Rat
Target Species: Mouse
Gene Accession No.: P01580
Gene ID (Entrez): 15978
Isotype: IgG1 κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
IFN gamma
Concentration:
0.2 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
BoIFNG; cytokine; EGK_03901; gamma interferon; gamma interferon precursor; gamma-interferon; gamma-interferon precursor (AA -23 to 143); H-IFN-g; IFG; IFI; IFN gamma; IFN ?; Ifng; IFN-g; IFNG2; IFN-gamma; IFN-gamma precursor; IFN-y; IFN?; Immune interferon; interferon gamma; interferon gamma precursor; interferon gamma type 2; Interferon y; Interferon ?; interferon, gamma; interferon-gama; interferon-gamma; Interferon-gamma level; interferon-gamma precursor; Interferon?; M-IFN-g; R-IFN-g
Gene Symbols:
IFNG
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
IFNG
Clone:
XMG1.2
Applications:
Flow Cytometry
Conjugate:
PE-eFluor 610
Host Species:
Rat
Target Species:
Mouse
Gene Accession No.:
P01580
Gene ID (Entrez):
15978
Isotype:
IgG1 κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: Ly-6G
Concentration: 0.2 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: Gr1; Gr-1; Ly6g; Ly-6G; ly-6G.1; lymphocyte antigen 6 complex, locus G; lymphocyte antigen 6G
Gene Symbols: Ly6g
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: Ly6g
Clone: 1A8-Ly6g
Applications: Flow Cytometry
Conjugate: PE-eFluor 610
Host Species: Rat
Target Species: Mouse
Gene Accession No.: P35461
Gene ID (Entrez): 546644
Isotype: IgG2a κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
Ly-6G
Concentration:
0.2 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
Gr1; Gr-1; Ly6g; Ly-6G; ly-6G.1; lymphocyte antigen 6 complex, locus G; lymphocyte antigen 6G
Gene Symbols:
Ly6g
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
Ly6g
Clone:
1A8-Ly6g
Applications:
Flow Cytometry
Conjugate:
PE-eFluor 610
Host Species:
Rat
Target Species:
Mouse
Gene Accession No.:
P35461
Gene ID (Entrez):
546644
Isotype:
IgG2a κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: HELIOS
Concentration: 5 μL/Test
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias: A730095J18Rik; ANF1A2; Helios; IKAROS family zinc finger 2; IKAROS family zinc finger 2 (Helios); ikaros family zinc finger protein 2; Ikzf2; Zfpn1a2; zinc finger DNA binding protein Helios; zinc finger protein Helios; zinc finger protein, subfamily 1A, 2 (Helios); ZNF1A2; Znfn1a2
Gene Symbols: IKZF2
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: IKZF2
Clone: 22F6
Applications: Flow Cytometry
Conjugate: PE-eFluor 610
Host Species: Armenian Hamster
Target Species: Canine, Human, Mouse
Gene Accession No.: P81183, Q9UKS7
Gene ID (Entrez): 22779, 22807, 488510
Isotype: IgG
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
HELIOS
Concentration:
5 μL/Test
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias:
A730095J18Rik; ANF1A2; Helios; IKAROS family zinc finger 2; IKAROS family zinc finger 2 (Helios); ikaros family zinc finger protein 2; Ikzf2; Zfpn1a2; zinc finger DNA binding protein Helios; zinc finger protein Helios; zinc finger protein, subfamily 1A, 2 (Helios); ZNF1A2; Znfn1a2
Gene Symbols:
IKZF2
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
IKZF2
Clone:
22F6
Applications:
Flow Cytometry
Conjugate:
PE-eFluor 610
Host Species:
Armenian Hamster
Target Species:
Canine, Human, Mouse
Gene Accession No.:
P81183, Q9UKS7
Gene ID (Entrez):
22779, 22807, 488510
Isotype:
IgG
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: CD279 (PD-1)
Concentration: 0.2 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: CD279; EGK_05005; hPD1; hPD-1; hPD-l; hSLE1; Ly101; mPD-1; PD1; PD-1; Pdc1; Pdcd1; programmed cell death 1; programmed cell death 1 protein; programmed cell death protein 1; programmed cell death protein 1-like; programmed death 1; Protein PD1; protein PD-1; sCD279; SLEB2; soluble CD279; systemic lupus erythematosus susceptibility 2
Gene Symbols: Pdcd1
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: Pdcd1
Clone: J43
Applications: Flow Cytometry
Conjugate: PE-eFluor 610
Host Species: Armenian Hamster
Target Species: Mouse
Gene Accession No.: Q02242
Gene ID (Entrez): 18566
Isotype: IgG
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
CD279 (PD-1)
Concentration:
0.2 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
CD279; EGK_05005; hPD1; hPD-1; hPD-l; hSLE1; Ly101; mPD-1; PD1; PD-1; Pdc1; Pdcd1; programmed cell death 1; programmed cell death 1 protein; programmed cell death protein 1; programmed cell death protein 1-like; programmed death 1; Protein PD1; protein PD-1; sCD279; SLEB2; soluble CD279; systemic lupus erythematosus susceptibility 2
Gene Symbols:
Pdcd1
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
Pdcd1
Clone:
J43
Applications:
Flow Cytometry
Conjugate:
PE-eFluor 610
Host Species:
Armenian Hamster
Target Species:
Mouse
Gene Accession No.:
Q02242
Gene ID (Entrez):
18566
Isotype:
IgG
Purification Method:
Affinity chromatography
Product Type:
Antibody