7092270
CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH5), PE-Cyanine7, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
CD274 (PD-L1, B7-H1)
Concentration
0.2 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Alias
A530045L16Rik; B7 homolog 1; B7-H; B7h1; B7-H1; Cd274; CD274 antigen; CD274 molecule; PDCD1 ligand 1; Pdcd1l1; Pdcd1lg1; Pdl1; PD-L1; pdl-1; Programmed cell death 1 ligand 1; programmed death ligand 1; RGD1566211; sCD274; soluble CD274; soluble PD L1; sPD L1
Gene Symbols
Cd274
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
Cd274
Clone
MIH5
Applications
Flow Cytometry
Conjugate
PE-Cyanine7
Host Species
Rat
Target Species
Mouse
Gene Accession No.
Q9EP73
Gene ID (Entrez)
60533
Isotype
IgG2a λ
Purification Method
Affinity chromatography
Product Type
Antibody
Related Products
Description
- Description: The MIH5 monoclonal antibody reacts with mouse B7-H1, also known as PD-L1
- B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily
- B7-H1 is expressed on a majority of leukocytes including T, B, NK and DC
- B7-H1 is a ligand for PD-1
- Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses
- MIH5 is reported to be a blocking antibody
- Applications Reported: This MIH5 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This MIH5 antibody has been tested by flow cytometric analysis of mouse splenocytes
- This can be used at less than or equal to 0.125 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- Light sensitivity: This tandem dye is sensitive to photo-induced oxidation
- Please protect this vial and stained samples from light
- Fixation: Samples can be stored in IC Fixation Buffer (cat
- 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat
- Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a recently described B7 family member
- To date, one specific receptor has been identified that can be ligated by PD-L1
- This receptor, programmed death receptor 1 (PD-1), has been shown to negatively regulate T-cell receptor (TCR) signaling
- Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production
- PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation
- Thus, PD-L1 might play an important role in tumor immune evasion.
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Antigen: CD274 (PD-L1, B7-H1)
Concentration: 0.2 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: A530045L16Rik; B7 homolog 1; B7-H; B7h1; B7-H1; Cd274; CD274 antigen; CD274 molecule; PDCD1 ligand 1; Pdcd1l1; Pdcd1lg1; Pdl1; PD-L1; pdl-1; Programmed cell death 1 ligand 1; programmed death ligand 1; RGD1566211; sCD274; soluble CD274; soluble PD L1; sPD L1
Gene Symbols: Cd274
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: Cd274
Clone: MIH5
Applications: Flow Cytometry
Conjugate: PE-Cyanine7
Host Species: Rat
Target Species: Mouse
Gene Accession No.: Q9EP73
Gene ID (Entrez): 60533
Isotype: IgG2a λ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
CD274 (PD-L1, B7-H1)
Concentration:
0.2 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
A530045L16Rik; B7 homolog 1; B7-H; B7h1; B7-H1; Cd274; CD274 antigen; CD274 molecule; PDCD1 ligand 1; Pdcd1l1; Pdcd1lg1; Pdl1; PD-L1; pdl-1; Programmed cell death 1 ligand 1; programmed death ligand 1; RGD1566211; sCD274; soluble CD274; soluble PD L1; sPD L1
Gene Symbols:
Cd274
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
Cd274
Clone:
MIH5
Applications:
Flow Cytometry
Conjugate:
PE-Cyanine7
Host Species:
Rat
Target Species:
Mouse
Gene Accession No.:
Q9EP73
Gene ID (Entrez):
60533
Isotype:
IgG2a λ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: Phospho-STAT5 (Tyr694)
Concentration: 5 μL/Test
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias: AA959963; mammary gland factor; mammary gland factor STAT5A; mammary gland factor; transcription factor; Mgf; Mpf; signal transducer and activator of transcription 5; signal transducer and activator of transcription 5a; signal transducer and activator of transcription factor 5A; STAT; STAT5; STAT5A; STAT5A, Mammary Gland Factor; STAT5A/MGF; STAT5B; STATA5
Gene Symbols: STAT5A
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: STAT5A
Clone: SRBCZX
Applications: Flow Cytometry
Conjugate: PE-Cyanine7
Host Species: Mouse
Target Species: Human, Mouse
Gene Accession No.: P42229, P42230
Gene ID (Entrez): 20850, 6776
Isotype: IgG1 κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
Phospho-STAT5 (Tyr694)
Concentration:
5 μL/Test
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias:
AA959963; mammary gland factor; mammary gland factor STAT5A; mammary gland factor; transcription factor; Mgf; Mpf; signal transducer and activator of transcription 5; signal transducer and activator of transcription 5a; signal transducer and activator of transcription factor 5A; STAT; STAT5; STAT5A; STAT5A, Mammary Gland Factor; STAT5A/MGF; STAT5B; STATA5
Gene Symbols:
STAT5A
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
STAT5A
Clone:
SRBCZX
Applications:
Flow Cytometry
Conjugate:
PE-Cyanine7
Host Species:
Mouse
Target Species:
Human, Mouse
Gene Accession No.:
P42229, P42230
Gene ID (Entrez):
20850, 6776
Isotype:
IgG1 κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: TIGIT
Concentration: 5 μL/Test
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias: ENSMUSG00000071552; T cell immunoreceptor with Ig and ITIM domains; T-cell immunoreceptor with Ig and ITIM domains; TIGIT; V-set and immunoglobulin domain containing 9; V-set and immunoglobulin domain-containing protein 9; V-set and transmembrane domain containing 3; V-set and transmembrane domain-containing protein 3; VSIG9; VSTM3; Washington University cell adhesion molecule; WUCAM
Gene Symbols: TIGIT
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: TIGIT
Clone: MBSA43
Applications: Flow Cytometry
Conjugate: PE-Cyanine7
Host Species: Mouse
Target Species: Human
Gene Accession No.: Q495A1
Gene ID (Entrez): 201633
Isotype: IgG1 κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
TIGIT
Concentration:
5 μL/Test
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias:
ENSMUSG00000071552; T cell immunoreceptor with Ig and ITIM domains; T-cell immunoreceptor with Ig and ITIM domains; TIGIT; V-set and immunoglobulin domain containing 9; V-set and immunoglobulin domain-containing protein 9; V-set and transmembrane domain containing 3; V-set and transmembrane domain-containing protein 3; VSIG9; VSTM3; Washington University cell adhesion molecule; WUCAM
Gene Symbols:
TIGIT
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
TIGIT
Clone:
MBSA43
Applications:
Flow Cytometry
Conjugate:
PE-Cyanine7
Host Species:
Mouse
Target Species:
Human
Gene Accession No.:
Q495A1
Gene ID (Entrez):
201633
Isotype:
IgG1 κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: CD274 (PD-L1, B7-H1)
Concentration: 0.2 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: A530045L16Rik; B7 homolog 1; B7-H; B7h1; B7-H1; Cd274; CD274 antigen; CD274 molecule; PDCD1 ligand 1; Pdcd1l1; Pdcd1lg1; Pdl1; PD-L1; pdl-1; Programmed cell death 1 ligand 1; programmed death ligand 1; RGD1566211; sCD274; soluble CD274; soluble PD L1; sPD L1
Gene Symbols: Cd274
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: Cd274
Clone: MIH5
Applications: Flow Cytometry
Conjugate: PerCP-eFluor 710
Host Species: Rat
Target Species: Mouse
Gene Accession No.: Q9EP73
Gene ID (Entrez): 60533
Isotype: IgG2a λ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
CD274 (PD-L1, B7-H1)
Concentration:
0.2 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
A530045L16Rik; B7 homolog 1; B7-H; B7h1; B7-H1; Cd274; CD274 antigen; CD274 molecule; PDCD1 ligand 1; Pdcd1l1; Pdcd1lg1; Pdl1; PD-L1; pdl-1; Programmed cell death 1 ligand 1; programmed death ligand 1; RGD1566211; sCD274; soluble CD274; soluble PD L1; sPD L1
Gene Symbols:
Cd274
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
Cd274
Clone:
MIH5
Applications:
Flow Cytometry
Conjugate:
PerCP-eFluor 710
Host Species:
Rat
Target Species:
Mouse
Gene Accession No.:
Q9EP73
Gene ID (Entrez):
60533
Isotype:
IgG2a λ
Purification Method:
Affinity chromatography
Product Type:
Antibody