7092279
CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH5), PerCP-eFluor™ 710, eBioscience™, Invitrogen™
Rat Monoclonal Antibody
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
CD274 (PD-L1, B7-H1)
Concentration
0.2 mg/mL
Classification
Monoclonal
Form
Liquid
Regulatory Status
RUO
Formulation
PBS with 0.09% sodium azide; pH 7.2
Gene Alias
A530045L16Rik; B7 homolog 1; B7-H; B7h1; B7-H1; Cd274; CD274 antigen; CD274 molecule; PDCD1 ligand 1; Pdcd1l1; Pdcd1lg1; Pdl1; PD-L1; pdl-1; Programmed cell death 1 ligand 1; programmed death ligand 1; RGD1566211; sCD274; soluble CD274; soluble PD L1; sPD L1
Gene Symbols
Cd274
Primary or Secondary
Primary
Content And Storage
4° C, store in dark, DO NOT FREEZE!
Gene
Cd274
Clone
MIH5
Applications
Flow Cytometry
Conjugate
PerCP-eFluor 710
Host Species
Rat
Target Species
Mouse
Gene Accession No.
Q9EP73
Gene ID (Entrez)
60533
Isotype
IgG2a λ
Purification Method
Affinity chromatography
Product Type
Antibody
Related Products
Description
- Description: The MIH5 monoclonal antibody reacts with mouse B7-H1, also known as PD-L1
- B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily
- B7-H1 is expressed on a majority of leukocytes including T, B, NK and DC
- B7-H1 is a ligand for PD-1
- Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses
- MIH5 is reported to be a blocking antibody
- Applications Reported: This MIH5 antibody has been reported for use in flow cytometric analysis
- Applications Tested: This MIH5 antibody has been tested by flow cytometric analysis of mouse splenocytes
- This can be used at less than or equal to 0.125 μg per test
- A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL
- Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test
- It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest
- PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5
- We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative
- Please make sure that your instrument is capable of detecting this fluorochrome
- Fixation: Samples can be stored in IC Fixation Buffer (cat
- Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a recently described B7 family member
- To date, one specific receptor has been identified that can be ligated by PD-L1
- This receptor, programmed death receptor 1 (PD-1), has been shown to negatively regulate T-cell receptor (TCR) signaling
- Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production
- PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation
- Thus, PD-L1 might play an important role in tumor immune evasion.
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Antigen: CD274 (PD-L1, B7-H1)
Concentration: 0.2 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: A530045L16Rik; B7 homolog 1; B7-H; B7h1; B7-H1; Cd274; CD274 antigen; CD274 molecule; PDCD1 ligand 1; Pdcd1l1; Pdcd1lg1; Pdl1; PD-L1; pdl-1; Programmed cell death 1 ligand 1; programmed death ligand 1; RGD1566211; sCD274; soluble CD274; soluble PD L1; sPD L1
Gene Symbols: Cd274
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: Cd274
Clone: MIH5
Applications: Flow Cytometry
Conjugate: PerCP-eFluor 710
Host Species: Rat
Target Species: Mouse
Gene Accession No.: Q9EP73
Gene ID (Entrez): 60533
Isotype: IgG2a λ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
CD274 (PD-L1, B7-H1)
Concentration:
0.2 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
A530045L16Rik; B7 homolog 1; B7-H; B7h1; B7-H1; Cd274; CD274 antigen; CD274 molecule; PDCD1 ligand 1; Pdcd1l1; Pdcd1lg1; Pdl1; PD-L1; pdl-1; Programmed cell death 1 ligand 1; programmed death ligand 1; RGD1566211; sCD274; soluble CD274; soluble PD L1; sPD L1
Gene Symbols:
Cd274
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
Cd274
Clone:
MIH5
Applications:
Flow Cytometry
Conjugate:
PerCP-eFluor 710
Host Species:
Rat
Target Species:
Mouse
Gene Accession No.:
Q9EP73
Gene ID (Entrez):
60533
Isotype:
IgG2a λ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: Phospho-ERK1/2 (Thr202, Tyr204)
Concentration: 5 μL/Test
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias: 12559; 9030612K14Rik; AA407128; AU018647; BcDNA:RE08694; C78273; CG12559; CG12559-PA; CG12559-PC; CG12559-PD; CG12559-PE; CG12559-PG; CG12559-PH; CG12559-PI; CG18732; CT34260; CT39192; DERK; D-ERK; DERK-A; Diphospho-ERK; dm-dpERK; Dmel\CG12559; Dmel_CG12559; DmErk; DmERKA; DmERK-A; DmMAPK; dpERK; dp-ERK; dpERK1; dpMAPK; drosophila ERK; Dsor2; E(sina)7; EC2-1; EK2-1; enhancer of seven in absentia 7; ERK; Erk MAP kinase; Erk/Map kinase; ERK/rolled; Erk1; Erk-1; erk1 erk2; Erk1/2; ERK1b; ERK2; ERK-2; ERKA; ERK-A; ERT1; ERT2; Esrk1; extracellular signal-regulated kinase; extracellular signal-regulated kinase (ERK2); extracellular signal-regulated kinase 1; Extracellular signal-regulated kinase 2; extracellular signal-regulated kinase A; extracellular signal-regulated kinase-1; extracellular signal-regulated kinase-2; extracellular signal-related kinase 1; Extracellular-regulated kinase A; extracellular-signal-regulated kinase 2; Extracellular-signal-related kinase A; EY2-2; fi06b09; GroupII; HS44KDAP; HUMKER1A; I79_009500; I79_018350; insulin-stimulated MAP2 kinase; l(2)41Ac; l(2R)EMS45-39; M phase MAP kinase; MAP kinase; MAP kinase 1; MAP kinase 2; MAP kinase 3; MAP kinase isoform p42; MAP kinase isoform p44; MapK; MAP-k; MAPK 1; MAPK 2; MAPK 3; MAPK1; mapk1.S; mapk1a; mapk1-a; mapk1-b; mapk2; mapk3; MAP-kinase; MBP kinase; Microtubule-associated protein 2 kinase; mitogen activated protein kinase; mitogen activated protein kinase 1; mitogen activated protein kinase 3; mitogen-activated 3; mitogen-activated protein kinase; mitogen-activated protein kinase 1; mitogen-activated protein kinase 1 S homeolog; mitogen-activated protein kinase 1a; mitogen-activated protein kinase 2; Mitogen-activated protein kinase 3; Mitogen-activated protein kinase ERK-A; MNK1; mpk1; Mtap2k; Myelin basic protein kinase; Myelin xP42 protein kinase; p38; p40; p41; p41mapk; p42; p42 MAP Kinase; P42MAPK; p42-MAPK; p44; p44 MAP kinase; p44erk1; p44-ERK1; p44mapk; p44-MAPK; pERK; p-ERK; phospho-ERK; pMapK; pp42/MAP kinase; Prkm1; PRKM2; Prkm3; protein kinase; Protein rolled; protein tyrosine kinase ERK2; RE08694p; RL; rl/ERK; rl/MAPK; rll; rl-PA; rl-PC; rl-PD; rl-PE; rl-PG; rl-PH; rl-PI; roll; rolled; sem; sevenmaker; SR2-1; Su(Raf)2B; wu:fi06b09; XELAEV_18010534mg; xp42; zERK1; zERK2
Gene Symbols: MAPK1, MAPK3
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: __
Clone: MILAN8R
Applications: Flow Cytometry
Conjugate: PerCP-eFluor 710
Host Species: Mouse
Target Species: Human, Mouse
Gene Accession No.: P27361, P28482, P63085, Q63844
Gene ID (Entrez): 26413, 26417, 5594, 5595
Isotype: IgG1 κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
Phospho-ERK1/2 (Thr202, Tyr204)
Concentration:
5 μL/Test
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Gene Alias:
12559; 9030612K14Rik; AA407128; AU018647; BcDNA:RE08694; C78273; CG12559; CG12559-PA; CG12559-PC; CG12559-PD; CG12559-PE; CG12559-PG; CG12559-PH; CG12559-PI; CG18732; CT34260; CT39192; DERK; D-ERK; DERK-A; Diphospho-ERK; dm-dpERK; Dmel\CG12559; Dmel_CG12559; DmErk; DmERKA; DmERK-A; DmMAPK; dpERK; dp-ERK; dpERK1; dpMAPK; drosophila ERK; Dsor2; E(sina)7; EC2-1; EK2-1; enhancer of seven in absentia 7; ERK; Erk MAP kinase; Erk/Map kinase; ERK/rolled; Erk1; Erk-1; erk1 erk2; Erk1/2; ERK1b; ERK2; ERK-2; ERKA; ERK-A; ERT1; ERT2; Esrk1; extracellular signal-regulated kinase; extracellular signal-regulated kinase (ERK2); extracellular signal-regulated kinase 1; Extracellular signal-regulated kinase 2; extracellular signal-regulated kinase A; extracellular signal-regulated kinase-1; extracellular signal-regulated kinase-2; extracellular signal-related kinase 1; Extracellular-regulated kinase A; extracellular-signal-regulated kinase 2; Extracellular-signal-related kinase A; EY2-2; fi06b09; GroupII; HS44KDAP; HUMKER1A; I79_009500; I79_018350; insulin-stimulated MAP2 kinase; l(2)41Ac; l(2R)EMS45-39; M phase MAP kinase; MAP kinase; MAP kinase 1; MAP kinase 2; MAP kinase 3; MAP kinase isoform p42; MAP kinase isoform p44; MapK; MAP-k; MAPK 1; MAPK 2; MAPK 3; MAPK1; mapk1.S; mapk1a; mapk1-a; mapk1-b; mapk2; mapk3; MAP-kinase; MBP kinase; Microtubule-associated protein 2 kinase; mitogen activated protein kinase; mitogen activated protein kinase 1; mitogen activated protein kinase 3; mitogen-activated 3; mitogen-activated protein kinase; mitogen-activated protein kinase 1; mitogen-activated protein kinase 1 S homeolog; mitogen-activated protein kinase 1a; mitogen-activated protein kinase 2; Mitogen-activated protein kinase 3; Mitogen-activated protein kinase ERK-A; MNK1; mpk1; Mtap2k; Myelin basic protein kinase; Myelin xP42 protein kinase; p38; p40; p41; p41mapk; p42; p42 MAP Kinase; P42MAPK; p42-MAPK; p44; p44 MAP kinase; p44erk1; p44-ERK1; p44mapk; p44-MAPK; pERK; p-ERK; phospho-ERK; pMapK; pp42/MAP kinase; Prkm1; PRKM2; Prkm3; protein kinase; Protein rolled; protein tyrosine kinase ERK2; RE08694p; RL; rl/ERK; rl/MAPK; rll; rl-PA; rl-PC; rl-PD; rl-PE; rl-PG; rl-PH; rl-PI; roll; rolled; sem; sevenmaker; SR2-1; Su(Raf)2B; wu:fi06b09; XELAEV_18010534mg; xp42; zERK1; zERK2
Gene Symbols:
MAPK1, MAPK3
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
__
Clone:
MILAN8R
Applications:
Flow Cytometry
Conjugate:
PerCP-eFluor 710
Host Species:
Mouse
Target Species:
Human, Mouse
Gene Accession No.:
P27361, P28482, P63085, Q63844
Gene ID (Entrez):
26413, 26417, 5594, 5595
Isotype:
IgG1 κ
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: CD279 (PD-1)
Concentration: 0.2 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: CD279; EGK_05005; hPD1; hPD-1; hPD-l; hSLE1; Ly101; mPD-1; PD1; PD-1; Pdc1; Pdcd1; programmed cell death 1; programmed cell death 1 protein; programmed cell death protein 1; programmed cell death protein 1-like; programmed death 1; Protein PD1; protein PD-1; sCD279; SLEB2; soluble CD279; systemic lupus erythematosus susceptibility 2
Gene Symbols: Pdcd1
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: Pdcd1
Clone: J43
Applications: Flow Cytometry
Conjugate: PerCP-eFluor 710
Host Species: Armenian Hamster
Target Species: Mouse
Gene Accession No.: Q02242
Gene ID (Entrez): 18566
Isotype: IgG
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
CD279 (PD-1)
Concentration:
0.2 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
CD279; EGK_05005; hPD1; hPD-1; hPD-l; hSLE1; Ly101; mPD-1; PD1; PD-1; Pdc1; Pdcd1; programmed cell death 1; programmed cell death 1 protein; programmed cell death protein 1; programmed cell death protein 1-like; programmed death 1; Protein PD1; protein PD-1; sCD279; SLEB2; soluble CD279; systemic lupus erythematosus susceptibility 2
Gene Symbols:
Pdcd1
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
Pdcd1
Clone:
J43
Applications:
Flow Cytometry
Conjugate:
PerCP-eFluor 710
Host Species:
Armenian Hamster
Target Species:
Mouse
Gene Accession No.:
Q02242
Gene ID (Entrez):
18566
Isotype:
IgG
Purification Method:
Affinity chromatography
Product Type:
Antibody
Antigen: CD117 (c-Kit)
Concentration: 0.2 mg/mL
Classification: Monoclonal
Form: Liquid
Regulatory Status: RUO
Formulation: PBS with 0.09% sodium azide; pH 7.2
Gene Alias: belly-spot; Bs; CD117; ckit; C-Kit; c-KIT gene1; c-kit protein; c-kit proto-oncogene protein; c-kit receptor; c-kit receptor tyrosine kinase; dominant spotting; Dominant white spotting; Fdc; Gsfsco1; Gsfsco5; Gsfsow3; KIT; kit oncogene; KIT proto-oncogene receptor tyrosine kinase; KIT proto-oncogene, receptor tyrosine kinase; mast cell growth factor receptor; mast/stem cell growth factor receptor; mast/stem cell growth factor receptor Kit; MGF; p145 c-kit; PBT; Piebald trait protein; protein kinase; proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; receptor tyrosine kinase c-kit; receptor tyrosine kinase type III; SCFR; SCO1; SCO5; Sl; soluble KIT variant 1; SOW3; spotted sterile male; Ssm; Steel Factor Receptor; Tr-kit; tyrosine kinase receptor; tyrosine kinase receptor c-KIT; tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein; W
Gene Symbols: KIT
Primary or Secondary: Primary
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Gene: KIT
Clone: 2B8
Applications: Flow Cytometry
Conjugate: APC-eFluor 780
Host Species: Rat
Target Species: Mouse, Porcine
Gene Accession No.: P05532, Q2HWD6
Gene ID (Entrez): 16590, 396810
Isotype: IgG2b κ
Purification Method: Affinity chromatography
Product Type: Antibody
Antigen:
CD117 (c-Kit)
Concentration:
0.2 mg/mL
Classification:
Monoclonal
Form:
Liquid
Regulatory Status:
RUO
Formulation:
PBS with 0.09% sodium azide; pH 7.2
Gene Alias:
belly-spot; Bs; CD117; ckit; C-Kit; c-KIT gene1; c-kit protein; c-kit proto-oncogene protein; c-kit receptor; c-kit receptor tyrosine kinase; dominant spotting; Dominant white spotting; Fdc; Gsfsco1; Gsfsco5; Gsfsow3; KIT; kit oncogene; KIT proto-oncogene receptor tyrosine kinase; KIT proto-oncogene, receptor tyrosine kinase; mast cell growth factor receptor; mast/stem cell growth factor receptor; mast/stem cell growth factor receptor Kit; MGF; p145 c-kit; PBT; Piebald trait protein; protein kinase; proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; receptor tyrosine kinase c-kit; receptor tyrosine kinase type III; SCFR; SCO1; SCO5; Sl; soluble KIT variant 1; SOW3; spotted sterile male; Ssm; Steel Factor Receptor; Tr-kit; tyrosine kinase receptor; tyrosine kinase receptor c-KIT; tyrosine-protein kinase Kit; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein; W
Gene Symbols:
KIT
Primary or Secondary:
Primary
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Gene:
KIT
Clone:
2B8
Applications:
Flow Cytometry
Conjugate:
APC-eFluor 780
Host Species:
Rat
Target Species:
Mouse, Porcine
Gene Accession No.:
P05532, Q2HWD6
Gene ID (Entrez):
16590, 396810
Isotype:
IgG2b κ
Purification Method:
Affinity chromatography
Product Type:
Antibody