7105403

Myeloid Cell Marker Antibody (SPM298), Novus Biologicals™

Mouse Monoclonal Antibody

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

Myeloid Cell Marker

Dilution

Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Formulation

PBS with 0.05% BSA. with 0.05% Sodium Azide

Immunogen

Human peripheral blood mononuclear cells

Primary or Secondary

Primary

Content And Storage

Store at 4C.

Molecular Weight of Antigen

183 kDa

Clone

SPM298

Applications

Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)

Conjugate

Unconjugated

Host Species

Mouse

Target Species

Human

Gene Accession No.

Unkonwn

Isotype

IgG1

Purification Method

Protein A purified

Test Specificity

Recognizes 183kDa protein with DNA-binding characteristics, which is identified as a myeloid specific antigen. BM-1 reacts with myeloid precursor cells and granulocytes in bone marrow. Its antigen appears to be restricted to M2 and M3 acute myelogenous leukemia (AML) subtypes. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. BM-1 and BM-2 antibodies react with early precursor and mature forms of human myeloid cells. BM-1 MAb is useful in the identification of myelogenous leukemias, distinguishing granulocytic sarcomas from lymphoid malignancies and also in the study of differentiation and transformation of human myeloid cells. The biological function of this antigen is not clear, although it has been proposed that BM-1 may play a role in the differentiation of myeloid cells.

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Description

  • Myeloid Cell Marker Monoclonal specifically detects Myeloid Cell Marker in Human samples
  • It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

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Img

Fischer Scientific

7105403

Mouse Monoclonal Antibody...


Antigen:
Myeloid Cell Marker

Dilution:
Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide

Immunogen:
Human peripheral blood mononuclear cells

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
183 kDa

Clone:
SPM298

Applications:
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)

Conjugate:
Unconjugated

Host Species:
Mouse

Target Species:
Human

Gene Accession No.:
Unkonwn

Isotype:
IgG1

Purification Method:
Protein A purified

Test Specificity:
Recognizes 183kDa protein with DNA-binding characteristics, which is identified as a myeloid specific antigen. BM-1 reacts with myeloid precursor cells and granulocytes in bone marrow. Its antigen appears to be restricted to M2 and M3 acute myelogenous leukemia (AML) subtypes. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. BM-1 and BM-2 antibodies react with early precursor and mature forms of human myeloid cells. BM-1 MAb is useful in the identification of myelogenous leukemias, distinguishing granulocytic sarcomas from lymphoid malignancies and also in the study of differentiation and transformation of human myeloid cells. The biological function of this antigen is not clear, although it has been proposed that BM-1 may play a role in the differentiation of myeloid cells.

Img

Fischer Scientific

7105404

Mouse Monoclonal Antibody has been ...


Antigen:
MyoD

Dilution:
Western Blot 0.25-0.5ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide

Immunogen:
Recombinant mouse MyoD1 protein

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
45 kDa

Clone:
SPM427

Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Host Species:
Mouse

Target Species:
Human, Mouse, Rat, Chicken

Gene Accession No.:
P15172

Isotype:
IgG1 κ

Purification Method:
Protein A purified

Test Specificity:
Recognizes a phosphor-protein of 45kDa, identified as MyoD1. The epitope of this MAb maps between amino acid 180-189 in the C-terminal of mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilms tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewings sarcomas and alveolar soft part sarcomas.

Img

Fischer Scientific

7105405

Mouse Monoclonal Antibody has been ...


Antigen:
MyoD

Dilution:
Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunohistochemistry-Paraffin 0.5-1.0ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide

Immunogen:
Recombinant human MyoD1 protein

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
45 kDa

Clone:
MYD712

Applications:
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Host Species:
Mouse

Target Species:
Human

Gene Accession No.:
P15172

Isotype:
IgG1 κ

Purification Method:
Protein A purified

Test Specificity:
Recognizes a phosphor-protein of 45kDa, identified as MyoD1. This MAb does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm s tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing s sarcomas and alveolar soft part sarcomas.

Img

Fischer Scientific

7105405

Mouse Monoclonal Antibody has been ...


Antigen:
MyoD

Dilution:
Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunohistochemistry-Paraffin 0.5-1.0ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide

Immunogen:
Recombinant human MyoD1 protein

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
45 kDa

Clone:
MYD712

Applications:
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Host Species:
Mouse

Target Species:
Human

Gene Accession No.:
P15172

Isotype:
IgG1 κ

Purification Method:
Protein A purified

Test Specificity:
Recognizes a phosphor-protein of 45kDa, identified as MyoD1. This MAb does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm s tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing s sarcomas and alveolar soft part sarcomas.