7105595

GFAP Mouse, Clone: SPM248, Novus Biologicals™

Mouse Monoclonal Antibody has been used in 2 publications

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Antigen

GFAP

Dilution

Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml

Classification

Monoclonal

Form

Purified

Regulatory Status

RUO

Target Species

Human, Mouse, Rat, Porcine, Bovine, Chicken, Rabbit

Gene Accession No.

P14136

Gene ID (Entrez)

2670

Immunogen

GFAP isolated from pig spinal cord

Primary or Secondary

Primary

Content And Storage

Store at 4C.

Molecular Weight of Antigen

50 kDa

Clone

SPM248

Applications

Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Conjugate

Unconjugated

Host Species

Mouse

Research Discipline

Astrocyte Markers, Cancer, Cellular Markers, Cytoskeleton Markers, Neuronal Stem Cell Markers, Neuroscience, Stem Cell Markers

Formulation

PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias

FLJ45472, GFAP astrocytes, glial fibrillary acidic protein

Gene Symbols

GFAP

Isotype

IgG1

Purification Method

Protein G purified

Test Specificity

This MAb recognizes a protein of ∼50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

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Description

  • GFAP Monoclonal specifically detects GFAP in Human, Mouse, Rat, Porcine, Bovine, Chicken, Rabbit samples
  • It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.

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7105595

Mouse Monoclonal Antibody has been ...


Antigen:
GFAP

Dilution:
Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human, Mouse, Rat, Porcine, Bovine, Chicken, Rabbit

Gene Accession No.:
P14136

Gene ID (Entrez):
2670

Immunogen:
GFAP isolated from pig spinal cord

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
50 kDa

Clone:
SPM248

Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Astrocyte Markers, Cancer, Cellular Markers, Cytoskeleton Markers, Neuronal Stem Cell Markers, Neuroscience, Stem Cell Markers

Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias:
FLJ45472, GFAP astrocytes, glial fibrillary acidic protein

Gene Symbols:
GFAP

Isotype:
IgG1

Purification Method:
Protein G purified

Test Specificity:
This MAb recognizes a protein of ∼50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

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Antigen:
Cytokeratin 18

Dilution:
Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human, Bovine (Negative), Canine (Negative), Hamster (Negative), Mouse (Negative), Porcine (Negative), Rat (Negative)

Gene Accession No.:
P05783

Gene ID (Entrez):
3875

Immunogen:
PMC-42 human breast carcinoma cells

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
45 kDa

Clone:
SPM265

Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Cancer, Cell Biology, Cellular Markers, Cytoskeleton Markers

Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias:
Cell proliferation-inducing gene 46 protein, cell proliferation-inducing protein 46, CK-18, CYK18, cytokeratin 18, cytokeratin-18, K18, keratin 18, keratin, type I cytoskeletal 18, keratin-18

Gene Symbols:
KRT18

Isotype:
IgG1

Purification Method:
Protein G purified

Test Specificity:
This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.

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Antigen:
GFAP

Dilution:
Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human, Mouse, Rat, Porcine, Bovine, Chicken, Rabbit

Gene Accession No.:
P14136

Gene ID (Entrez):
2670

Immunogen:
GFAP isolated from pig spinal cord

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
50 kDa

Clone:
SPM507

Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Astrocyte Markers, Cancer, Cellular Markers, Cytoskeleton Markers, Neuronal Stem Cell Markers, Neuroscience, Stem Cell Markers

Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias:
FLJ45472, GFAP astrocytes, glial fibrillary acidic protein

Gene Symbols:
GFAP

Isotype:
IgG1

Purification Method:
Protein G purified

Test Specificity:
This MAb recognizes a protein of ∼50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

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Antigen:
Bcl-10

Dilution:
Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml

Classification:
Monoclonal

Form:
Purified

Regulatory Status:
RUO

Target Species:
Human

Gene Accession No.:
O95999

Gene ID (Entrez):
8915

Immunogen:
Human BCL10 recombinant protein (epitope aa122-168)

Primary or Secondary:
Primary

Content And Storage:
Store at 4C.

Molecular Weight of Antigen:
33 kDa

Clone:
SPM520

Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)

Conjugate:
Unconjugated

Host Species:
Mouse

Research Discipline:
Apoptosis, Cancer

Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide

Gene Alias:
B-cell CLL/lymphoma 10CIPERCLAPB-cell lymphoma/leukemia 10, Bcl-10, CARD-containing apoptotic signaling protein, CARD-containing molecule enhancing NF-kappa-B, CARD-containing proapoptotic protein, CARD-like apoptotic protein, CARMEN, caspase-recruiting domain-containing protein, cCARMEN, c-E10bcl-10, CED-3/ICH-1 prodomain homologous E10-like regulator, Cellular homolog of vCARMEN, cellular-E10, hCLAP, Mammalian CARD-containing adapter molecule E10, mE10CARD containing molecule enhancing NF-kB

Gene Symbols:
BCL10

Isotype:
IgG1 κ

Purification Method:
Protein A purified

Test Specificity:
This MAb labels subpopulations of normal B and T cells and is a useful tool for the sub-classification of lymphomas.