7105409
p27/Kip1 Mouse, Clone: SX53G8, Novus Biologicals™
Mouse Monoclonal Antibody has been used in 1 publication
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
p27/Kip1
Dilution
Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunohistochemistry-Paraffin 0.5-1.0ug/ml
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human, Mouse, Rat, Primate
Gene Accession No.
P46527
Gene ID (Entrez)
1027
Immunogen
Purified GST-p27 fusion protein of human origin
Primary or Secondary
Primary
Content And Storage
Store at 4C.
Clone
SX53G8
Applications
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Breast Cancer, Cancer, Cell Cycle and Replication, DNA Repair, Phospho Specific, Prostate Cancer, Tumor Suppressors
Formulation
PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias
CDKN4, cyclin-dependent kinase inhibitor 1B, cyclin-dependent kinase inhibitor 1B (p27, Kip1), Cyclin-dependent kinase inhibitor p27, KIP1P27KIP1, MEN1B, MEN4, p27Kip1
Gene Symbols
CDKN1B
Isotype
IgG1 κ
Purification Method
Protein A purified
Test Specificity
This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1. It functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix.
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Description
- Description p27/Kip1 Monoclonal specifically detects p27/Kip1 in Human, Mouse, Rat, Monkey samples
- It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin.
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Antigen: p27/Kip1
Dilution: Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunohistochemistry-Paraffin 0.5-1.0ug/ml
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Mouse, Rat, Primate
Gene Accession No.: P46527
Gene ID (Entrez): 1027
Immunogen: Purified GST-p27 fusion protein of human origin
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Clone: SX53G8
Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Breast Cancer, Cancer, Cell Cycle and Replication, DNA Repair, Phospho Specific, Prostate Cancer, Tumor Suppressors
Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias: CDKN4, cyclin-dependent kinase inhibitor 1B, cyclin-dependent kinase inhibitor 1B (p27, Kip1), Cyclin-dependent kinase inhibitor p27, KIP1P27KIP1, MEN1B, MEN4, p27Kip1
Gene Symbols: CDKN1B
Isotype: IgG1 κ
Purification Method: Protein A purified
Test Specificity: This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1. It functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix.
Antigen:
p27/Kip1
Dilution:
Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunohistochemistry-Paraffin 0.5-1.0ug/ml
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Mouse, Rat, Primate
Gene Accession No.:
P46527
Gene ID (Entrez):
1027
Immunogen:
Purified GST-p27 fusion protein of human origin
Primary or Secondary:
Primary
Content And Storage:
Store at 4C.
Clone:
SX53G8
Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Breast Cancer, Cancer, Cell Cycle and Replication, DNA Repair, Phospho Specific, Prostate Cancer, Tumor Suppressors
Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias:
CDKN4, cyclin-dependent kinase inhibitor 1B, cyclin-dependent kinase inhibitor 1B (p27, Kip1), Cyclin-dependent kinase inhibitor p27, KIP1P27KIP1, MEN1B, MEN4, p27Kip1
Gene Symbols:
CDKN1B
Isotype:
IgG1 κ
Purification Method:
Protein A purified
Test Specificity:
This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1. It functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix.
Antigen: p27/Kip1
Dilution: Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunohistochemistry-Paraffin 0.5-1.0ug/ml
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Mouse, Rat, Primate
Gene Accession No.: P46527
Gene ID (Entrez): 1027
Immunogen: Purified GST-p27 fusion protein of human origin
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Clone: SPM348
Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Breast Cancer, Cancer, Cell Cycle and Replication, DNA Repair, Phospho Specific, Prostate Cancer, Tumor Suppressors
Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias: CDKN4, cyclin-dependent kinase inhibitor 1B, cyclin-dependent kinase inhibitor 1B (p27, Kip1), Cyclin-dependent kinase inhibitor p27, KIP1P27KIP1, MEN1B, MEN4, p27Kip1
Gene Symbols: CDKN1B
Isotype: IgG1 κ
Purification Method: Protein A purified
Test Specificity: This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This MAb is excellent for staining of formalin-fixed tissues.
Antigen:
p27/Kip1
Dilution:
Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunohistochemistry-Paraffin 0.5-1.0ug/ml
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Mouse, Rat, Primate
Gene Accession No.:
P46527
Gene ID (Entrez):
1027
Immunogen:
Purified GST-p27 fusion protein of human origin
Primary or Secondary:
Primary
Content And Storage:
Store at 4C.
Clone:
SPM348
Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Breast Cancer, Cancer, Cell Cycle and Replication, DNA Repair, Phospho Specific, Prostate Cancer, Tumor Suppressors
Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias:
CDKN4, cyclin-dependent kinase inhibitor 1B, cyclin-dependent kinase inhibitor 1B (p27, Kip1), Cyclin-dependent kinase inhibitor p27, KIP1P27KIP1, MEN1B, MEN4, p27Kip1
Gene Symbols:
CDKN1B
Isotype:
IgG1 κ
Purification Method:
Protein A purified
Test Specificity:
This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. This MAb is excellent for staining of formalin-fixed tissues.
Antigen: p53
Dilution: Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunohistochemistry-Paraffin 0.5-1.0ug/ml
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Mouse (Negative), Rat (Negative)
Gene Accession No.: P04637
Gene ID (Entrez): 7157
Immunogen: Recombinant human wild-type p53 protein
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Clone: SPM589
Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Apoptosis, Cancer, Cell Cycle and Replication, Cellular Markers, Checkpoint signaling, Core ESC Like Genes, DNA Double Strand Break Repair, DNA Repair, HIF Target Genes, Hypoxia, Neuroscience, Neurotransmission, p53 Pathway, Phospho Specific, Stem Cell Markers, Transcription Factors and Regulators, Tumor Suppressors
Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias: Antigen NY-CO-13, FLJ92943, LFS1TRP53, p53, p53 tumor suppressor, P53cellular tumor antigen p53, Phosphoprotein p53, transformation-related protein 53, tumor protein p53, Tumor suppressor p53
Gene Symbols: TP53
Isotype: IgG2a
Purification Method: Protein A purified
Test Specificity: Recognizes a 53kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53 under denaturing and non-denaturing conditions. Its epitope maps within the N-terminus (aa 20-25) of p53 oncoprotein. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.
Antigen:
p53
Dilution:
Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunohistochemistry-Paraffin 0.5-1.0ug/ml
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Mouse (Negative), Rat (Negative)
Gene Accession No.:
P04637
Gene ID (Entrez):
7157
Immunogen:
Recombinant human wild-type p53 protein
Primary or Secondary:
Primary
Content And Storage:
Store at 4C.
Clone:
SPM589
Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Apoptosis, Cancer, Cell Cycle and Replication, Cellular Markers, Checkpoint signaling, Core ESC Like Genes, DNA Double Strand Break Repair, DNA Repair, HIF Target Genes, Hypoxia, Neuroscience, Neurotransmission, p53 Pathway, Phospho Specific, Stem Cell Markers, Transcription Factors and Regulators, Tumor Suppressors
Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias:
Antigen NY-CO-13, FLJ92943, LFS1TRP53, p53, p53 tumor suppressor, P53cellular tumor antigen p53, Phosphoprotein p53, transformation-related protein 53, tumor protein p53, Tumor suppressor p53
Gene Symbols:
TP53
Isotype:
IgG2a
Purification Method:
Protein A purified
Test Specificity:
Recognizes a 53kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53 under denaturing and non-denaturing conditions. Its epitope maps within the N-terminus (aa 20-25) of p53 oncoprotein. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.
Antigen: Pax7
Dilution: Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Mouse, Rat, Chicken, Zebrafish
Gene Accession No.: P23759
Gene ID (Entrez): 5081
Immunogen: Recombinant fragment (aa300-600) of human PAX7 protein
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Clone: PAX7/497
Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Apoptosis, Mesenchymal Stem Cell Markers, Stem Cell Markers
Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias: FLJ37460, HuP1, paired box 7, paired box gene 7, paired box homeotic gene 7, paired box protein Pax-7, paired domain gene 7, PAX7 transcriptional factor, PAX7B, RMS2
Gene Symbols: PAX7
Isotype: IgG1 κ
Purification Method: Protein A purified
Test Specificity: The Pax gene family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. In addition, the family is involved in a variety of signal transduction pathways in the adult organism. Mutations in the Pax family of proteins have been linked to disease and cancer in humans. Pax-7 is a protein specifically expressed in cultured satellite cell-derived myoblasts. In situ hybridization reveals that Pax-7 is also expressed in satellite cells residing in adult muscle. A chromosomal aberration in the gene encoding Pax-7 causes rhabdomyosarcoma 2 (RMS2) (also called alveolar rhabdomyosarcoma).
Antigen:
Pax7
Dilution:
Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Mouse, Rat, Chicken, Zebrafish
Gene Accession No.:
P23759
Gene ID (Entrez):
5081
Immunogen:
Recombinant fragment (aa300-600) of human PAX7 protein
Primary or Secondary:
Primary
Content And Storage:
Store at 4C.
Clone:
PAX7/497
Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Apoptosis, Mesenchymal Stem Cell Markers, Stem Cell Markers
Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias:
FLJ37460, HuP1, paired box 7, paired box gene 7, paired box homeotic gene 7, paired box protein Pax-7, paired domain gene 7, PAX7 transcriptional factor, PAX7B, RMS2
Gene Symbols:
PAX7
Isotype:
IgG1 κ
Purification Method:
Protein A purified
Test Specificity:
The Pax gene family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. In addition, the family is involved in a variety of signal transduction pathways in the adult organism. Mutations in the Pax family of proteins have been linked to disease and cancer in humans. Pax-7 is a protein specifically expressed in cultured satellite cell-derived myoblasts. In situ hybridization reveals that Pax-7 is also expressed in satellite cells residing in adult muscle. A chromosomal aberration in the gene encoding Pax-7 causes rhabdomyosarcoma 2 (RMS2) (also called alveolar rhabdomyosarcoma).