7105442
Siglec-3/CD33 Antibody (WM53), Novus Biologicals™
Mouse Monoclonal Antibody has been used in 1 publication
Manufacturer: Fischer Scientific
The price for this product is unavailable. Please request a quote
Antigen
Siglec-3/CD33
Dilution
Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human
Gene Accession No.
P20138
Gene ID (Entrez)
945
Immunogen
Human AML cells
Primary or Secondary
Primary
Content And Storage
Store at 4C.
Molecular Weight of Antigen
67 kDa
Clone
WM53
Applications
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen)
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Immunology
Formulation
PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias
CD33 antigen, CD33 antigen (gp67), CD33 molecule, FLJ00391, myeloid cell surface antigen CD33, p67, sialic acid binding Ig-like lectin 3, Sialic acid-binding Ig-like lectin 3, SIGLEC-3, SIGLEC3gp67
Gene Symbols
CD33
Isotype
IgG1 κ
Purification Method
Protein A purified
Test Specificity
Recognizes a 67kDa glycoprotein, which is identified as CD33 (HLDA IV; WS Code M-505). CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immuno-receptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. CD33 is expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. It is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.
Related Products
Description
- Siglec-3/CD33 Monoclonal specifically detects Siglec-3/CD33 in Human samples
- It is validated for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Functional.
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Antigen: Siglec-3/CD33
Dilution: Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Accession No.: P20138
Gene ID (Entrez): 945
Immunogen: Human AML cells
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Molecular Weight of Antigen: 67 kDa
Clone: WM53
Applications: Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen)
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Immunology
Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias: CD33 antigen, CD33 antigen (gp67), CD33 molecule, FLJ00391, myeloid cell surface antigen CD33, p67, sialic acid binding Ig-like lectin 3, Sialic acid-binding Ig-like lectin 3, SIGLEC-3, SIGLEC3gp67
Gene Symbols: CD33
Isotype: IgG1 κ
Purification Method: Protein A purified
Test Specificity: Recognizes a 67kDa glycoprotein, which is identified as CD33 (HLDA IV; WS Code M-505). CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immuno-receptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. CD33 is expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. It is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.
Antigen:
Siglec-3/CD33
Dilution:
Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Accession No.:
P20138
Gene ID (Entrez):
945
Immunogen:
Human AML cells
Primary or Secondary:
Primary
Content And Storage:
Store at 4C.
Molecular Weight of Antigen:
67 kDa
Clone:
WM53
Applications:
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen)
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Immunology
Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias:
CD33 antigen, CD33 antigen (gp67), CD33 molecule, FLJ00391, myeloid cell surface antigen CD33, p67, sialic acid binding Ig-like lectin 3, Sialic acid-binding Ig-like lectin 3, SIGLEC-3, SIGLEC3gp67
Gene Symbols:
CD33
Isotype:
IgG1 κ
Purification Method:
Protein A purified
Test Specificity:
Recognizes a 67kDa glycoprotein, which is identified as CD33 (HLDA IV; WS Code M-505). CD33 is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immuno-receptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. CD33 is expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. It is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.
Antigen: CD34
Dilution: Western Blot 0.25-0.5ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Primate, Bovine (Negative), Canine (Negative), Rat (Negative), Sheep (Negative)
Gene Accession No.: P28906
Gene ID (Entrez): 947
Immunogen: Detergent solubilized vesicular suspension prepared from human term placenta
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Molecular Weight of Antigen: __
Clone: QBEnd/10
Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Adaptive Immunity, Breast Cancer, Cancer, Cell Biology, Cellular Markers, Endothelial Cell Markers, Hematopoietic Stem Cell Markers, Hypoxia, Immunology, Innate Immunity, Mast Cell Markers, Mesenchymal Stem Cell Markers, Myeloid Cell Markers, Neuronal Cell Markers, Neuroscience, Stem Cell Markers, Tumor Suppressors
Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias: CD34 antigenhematopoietic progenitor cell antigen CD34, CD34 molecule
Gene Symbols: CD34
Isotype: IgG1 κ
Purification Method: Protein A purified
Test Specificity: This MAb recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. On the basis of differential sensitivity to degradation by specific enzymes, epitopes of monoclonal antibodies to CD34 are classified into three main categories, class I, class II and class III. It is a class II antibody whose epitope is resistant to neuraminidase but sensitive to glycoprotease and chymopapain. CD34 expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, CD34 is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. Its expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells express this molecule more than the non-proliferating endothelial cells. A
Antigen:
CD34
Dilution:
Western Blot 0.25-0.5ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Primate, Bovine (Negative), Canine (Negative), Rat (Negative), Sheep (Negative)
Gene Accession No.:
P28906
Gene ID (Entrez):
947
Immunogen:
Detergent solubilized vesicular suspension prepared from human term placenta
Primary or Secondary:
Primary
Content And Storage:
Store at 4C.
Molecular Weight of Antigen:
__
Clone:
QBEnd/10
Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Adaptive Immunity, Breast Cancer, Cancer, Cell Biology, Cellular Markers, Endothelial Cell Markers, Hematopoietic Stem Cell Markers, Hypoxia, Immunology, Innate Immunity, Mast Cell Markers, Mesenchymal Stem Cell Markers, Myeloid Cell Markers, Neuronal Cell Markers, Neuroscience, Stem Cell Markers, Tumor Suppressors
Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias:
CD34 antigenhematopoietic progenitor cell antigen CD34, CD34 molecule
Gene Symbols:
CD34
Isotype:
IgG1 κ
Purification Method:
Protein A purified
Test Specificity:
This MAb recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. On the basis of differential sensitivity to degradation by specific enzymes, epitopes of monoclonal antibodies to CD34 are classified into three main categories, class I, class II and class III. It is a class II antibody whose epitope is resistant to neuraminidase but sensitive to glycoprotease and chymopapain. CD34 expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, CD34 is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. Its expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells express this molecule more than the non-proliferating endothelial cells. A
Antigen: CD34
Dilution: Western Blot 0.25-0.5ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Primate, Bovine (Negative), Canine (Negative), Rat (Negative), Sheep (Negative)
Gene Accession No.: P28906
Gene ID (Entrez): 947
Immunogen: Detergent solubilized vesicular suspension prepared from human term placenta
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Molecular Weight of Antigen: __
Clone: QBEnd/10
Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Adaptive Immunity, Breast Cancer, Cancer, Cell Biology, Cellular Markers, Endothelial Cell Markers, Hematopoietic Stem Cell Markers, Hypoxia, Immunology, Innate Immunity, Mast Cell Markers, Mesenchymal Stem Cell Markers, Myeloid Cell Markers, Neuronal Cell Markers, Neuroscience, Stem Cell Markers, Tumor Suppressors
Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias: CD34 antigenhematopoietic progenitor cell antigen CD34, CD34 molecule
Gene Symbols: CD34
Isotype: IgG1 κ
Purification Method: Protein A purified
Test Specificity: This MAb recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. On the basis of differential sensitivity to degradation by specific enzymes, epitopes of monoclonal antibodies to CD34 are classified into three main categories, class I, class II and class III. It is a class II antibody whose epitope is resistant to neuraminidase but sensitive to glycoprotease and chymopapain. CD34 expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, CD34 is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. Its expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells express this molecule more than the non-proliferating endothelial cells. A
Antigen:
CD34
Dilution:
Western Blot 0.25-0.5ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Primate, Bovine (Negative), Canine (Negative), Rat (Negative), Sheep (Negative)
Gene Accession No.:
P28906
Gene ID (Entrez):
947
Immunogen:
Detergent solubilized vesicular suspension prepared from human term placenta
Primary or Secondary:
Primary
Content And Storage:
Store at 4C.
Molecular Weight of Antigen:
__
Clone:
QBEnd/10
Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Adaptive Immunity, Breast Cancer, Cancer, Cell Biology, Cellular Markers, Endothelial Cell Markers, Hematopoietic Stem Cell Markers, Hypoxia, Immunology, Innate Immunity, Mast Cell Markers, Mesenchymal Stem Cell Markers, Myeloid Cell Markers, Neuronal Cell Markers, Neuroscience, Stem Cell Markers, Tumor Suppressors
Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias:
CD34 antigenhematopoietic progenitor cell antigen CD34, CD34 molecule
Gene Symbols:
CD34
Isotype:
IgG1 κ
Purification Method:
Protein A purified
Test Specificity:
This MAb recognizes a single chain, transmembrane, heavily glycosylated protein of 90-120kDa, which is identified as CD34. On the basis of differential sensitivity to degradation by specific enzymes, epitopes of monoclonal antibodies to CD34 are classified into three main categories, class I, class II and class III. It is a class II antibody whose epitope is resistant to neuraminidase but sensitive to glycoprotease and chymopapain. CD34 expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, CD34 is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. Its expression is also found in vascular endothelium. Additionally, it appears that proliferating endothelial cells express this molecule more than the non-proliferating endothelial cells. A
Antigen: CD34
Dilution: Western Blot 0.25-0.5ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Primate, Bovine (Negative), Canine (Negative), Rat (Negative), Sheep (Negative)
Gene Accession No.: P28906
Gene ID (Entrez): 947
Immunogen: Detergent solubilized vesicular suspension prepared from human term placenta
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Molecular Weight of Antigen: __
Clone: SPM123
Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Adaptive Immunity, Breast Cancer, Cancer, Cell Biology, Cellular Markers, Endothelial Cell Markers, Hematopoietic Stem Cell Markers, Hypoxia, Immunology, Innate Immunity, Mast Cell Markers, Mesenchymal Stem Cell Markers, Myeloid Cell Markers, Neuronal Cell Markers, Neuroscience, Stem Cell Markers, Tumor Suppressors
Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias: CD34 antigenhematopoietic progenitor cell antigen CD34, CD34 molecule
Gene Symbols: CD34
Isotype: IgG1 κ
Purification Method: Protein A purified
Test Specificity: __
Antigen:
CD34
Dilution:
Western Blot 0.25-0.5ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1.0ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Primate, Bovine (Negative), Canine (Negative), Rat (Negative), Sheep (Negative)
Gene Accession No.:
P28906
Gene ID (Entrez):
947
Immunogen:
Detergent solubilized vesicular suspension prepared from human term placenta
Primary or Secondary:
Primary
Content And Storage:
Store at 4C.
Molecular Weight of Antigen:
__
Clone:
SPM123
Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Adaptive Immunity, Breast Cancer, Cancer, Cell Biology, Cellular Markers, Endothelial Cell Markers, Hematopoietic Stem Cell Markers, Hypoxia, Immunology, Innate Immunity, Mast Cell Markers, Mesenchymal Stem Cell Markers, Myeloid Cell Markers, Neuronal Cell Markers, Neuroscience, Stem Cell Markers, Tumor Suppressors
Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias:
CD34 antigenhematopoietic progenitor cell antigen CD34, CD34 molecule
Gene Symbols:
CD34
Isotype:
IgG1 κ
Purification Method:
Protein A purified
Test Specificity:
__