7105512
EGF R/ErbB1 Mouse, Clone: H9B4, Novus Biologicals™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
EGFR
Dilution
Western Blot 0.5-1.0ug/ml, Simple Western 10 ug/ml, Immunoprecipitation 1-2ug/500ug protein lysate
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human, Mouse (Negative), Rat (Negative)
Gene Accession No.
P00533
Gene ID (Entrez)
1956
Immunogen
Purified EGFR from A431 cells.
Primary or Secondary
Primary
Content And Storage
Store at 4C.
Molecular Weight of Antigen
170 kDa
Clone
H9B4
Applications
Western Blot, Immunoprecipitation
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Cancer, Cell Biology, Cell Cycle and Replication, Growth and Development, Hypoxia, Signal Transduction, Tumor Suppressors, Tyrosine Kinases
Formulation
PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, EC 2.7.10, EC 2.7.10.1, epidermal growth factor receptor, epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog), ERBB, ErbB1, ERBB1PIG61, HER1, mENA, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
Gene Symbols
EGFR
Isotype
IgG1
Purification Method
Protein G purified
Test Specificity
This MAb reacts with a cytoplasmic domain of EGFR. EGFR is a type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, beta cellulin & epiregulin.
Related Products
Description
- EGFR Monoclonal specifically detects EGFR in Human, Mouse (Negative), Rat (Negative) samples
- It is validated for Western Blot, Simple Western, Immunoprecipitation, Protein Array.
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Antigen: EGFR
Dilution: Western Blot 0.5-1.0ug/ml, Simple Western 10 ug/ml, Immunoprecipitation 1-2ug/500ug protein lysate
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Mouse (Negative), Rat (Negative)
Gene Accession No.: P00533
Gene ID (Entrez): 1956
Immunogen: Purified EGFR from A431 cells.
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Molecular Weight of Antigen: 170 kDa
Clone: H9B4
Applications: Western Blot, Immunoprecipitation
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cancer, Cell Biology, Cell Cycle and Replication, Growth and Development, Hypoxia, Signal Transduction, Tumor Suppressors, Tyrosine Kinases
Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias: avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, EC 2.7.10, EC 2.7.10.1, epidermal growth factor receptor, epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog), ERBB, ErbB1, ERBB1PIG61, HER1, mENA, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
Gene Symbols: EGFR
Isotype: IgG1
Purification Method: Protein G purified
Test Specificity: This MAb reacts with a cytoplasmic domain of EGFR. EGFR is a type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, beta cellulin & epiregulin.
Antigen:
EGFR
Dilution:
Western Blot 0.5-1.0ug/ml, Simple Western 10 ug/ml, Immunoprecipitation 1-2ug/500ug protein lysate
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Mouse (Negative), Rat (Negative)
Gene Accession No.:
P00533
Gene ID (Entrez):
1956
Immunogen:
Purified EGFR from A431 cells.
Primary or Secondary:
Primary
Content And Storage:
Store at 4C.
Molecular Weight of Antigen:
170 kDa
Clone:
H9B4
Applications:
Western Blot, Immunoprecipitation
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Cancer, Cell Biology, Cell Cycle and Replication, Growth and Development, Hypoxia, Signal Transduction, Tumor Suppressors, Tyrosine Kinases
Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias:
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog, cell growth inhibiting protein 40, cell proliferation-inducing protein 61, EC 2.7.10, EC 2.7.10.1, epidermal growth factor receptor, epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog), ERBB, ErbB1, ERBB1PIG61, HER1, mENA, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
Gene Symbols:
EGFR
Isotype:
IgG1
Purification Method:
Protein G purified
Test Specificity:
This MAb reacts with a cytoplasmic domain of EGFR. EGFR is a type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, beta cellulin & epiregulin.
Antigen: Ku70/XRCC6
Dilution: Immunocytochemistry/Immunofluorescence 0.5-1.0ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Primate, Chicken (Negative), Mouse (Negative), Rat (Negative)
Gene Accession No.: P12956
Gene ID (Entrez): 2547
Immunogen: Nuclear extract of human HL-60 cells
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Molecular Weight of Antigen: 75 kDa
Clone: KU729
Applications: Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Breast Cancer, Cancer, DNA Double Strand Break Repair, DNA Repair, Non homologous end joining
Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias: 5'-deoxyribose-5-phosphate lyase Ku70, 5'-dRP lyase Ku70, 70 kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, CTC box binding factor 75 kDa subunit, CTC box-binding factor 75 kDa subunit, CTC75, CTCBF, D22S731, EC 3.6.4.-, EC 4.2.99.-, G22P1Ku70, Ku autoantigen p70 subunit, Ku autoantigen, 70kDa, KU70, ML8,70 kDa subunit, Thyroid-lupus autoantigen, thyroid-lupus autoantigen p70, TLAAD22S671, X-ray repair complementing defective repair in Chinese hamster cells 6DNA repair protein XRCC6, X-ray repair cross-complementing protein 6
Gene Symbols: XRCC6
Isotype: IgG1 κ
Purification Method: Protein A purified
Test Specificity: Recognizes a dimer of two proteins of 70kDa and ∼80kDa, identified as two subunits of Ku. This MAb recognizes a conformational epitope of p70/p80 dimer, which is destroyed during Western blotting. The p70/p80 dimer is important for function of a 460kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
Antigen:
Ku70/XRCC6
Dilution:
Immunocytochemistry/Immunofluorescence 0.5-1.0ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Primate, Chicken (Negative), Mouse (Negative), Rat (Negative)
Gene Accession No.:
P12956
Gene ID (Entrez):
2547
Immunogen:
Nuclear extract of human HL-60 cells
Primary or Secondary:
Primary
Content And Storage:
Store at 4C.
Molecular Weight of Antigen:
75 kDa
Clone:
KU729
Applications:
Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Breast Cancer, Cancer, DNA Double Strand Break Repair, DNA Repair, Non homologous end joining
Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias:
5'-deoxyribose-5-phosphate lyase Ku70, 5'-dRP lyase Ku70, 70 kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 1, ATP-dependent DNA helicase II 70 kDa subunit, CTC box binding factor 75 kDa subunit, CTC box-binding factor 75 kDa subunit, CTC75, CTCBF, D22S731, EC 3.6.4.-, EC 4.2.99.-, G22P1Ku70, Ku autoantigen p70 subunit, Ku autoantigen, 70kDa, KU70, ML8,70 kDa subunit, Thyroid-lupus autoantigen, thyroid-lupus autoantigen p70, TLAAD22S671, X-ray repair complementing defective repair in Chinese hamster cells 6DNA repair protein XRCC6, X-ray repair cross-complementing protein 6
Gene Symbols:
XRCC6
Isotype:
IgG1 κ
Purification Method:
Protein A purified
Test Specificity:
Recognizes a dimer of two proteins of 70kDa and ∼80kDa, identified as two subunits of Ku. This MAb recognizes a conformational epitope of p70/p80 dimer, which is destroyed during Western blotting. The p70/p80 dimer is important for function of a 460kDa DNA-dependent protein kinase. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
Antigen: IgG
Dilution: Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Accession No.: P01857
Gene ID (Entrez): 3500
Immunogen: Purified human Ig Gamma Chain
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Molecular Weight of Antigen: 75 kDa
Clone: ICO-97
Applications: Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen)
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Immunology
Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias: immunoglobulin heavy constant gamma 1 (G1m marker)
Gene Symbols: IGHG1
Isotype: IgG1 κ
Purification Method: Protein A purified
Test Specificity: Recognizes a protein of 75kDa, identified as gamma heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), u (IgM), Epsilon (IgE), or Delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Antigen:
IgG
Dilution:
Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Accession No.:
P01857
Gene ID (Entrez):
3500
Immunogen:
Purified human Ig Gamma Chain
Primary or Secondary:
Primary
Content And Storage:
Store at 4C.
Molecular Weight of Antigen:
75 kDa
Clone:
ICO-97
Applications:
Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Frozen)
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Immunology
Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias:
immunoglobulin heavy constant gamma 1 (G1m marker)
Gene Symbols:
IGHG1
Isotype:
IgG1 κ
Purification Method:
Protein A purified
Test Specificity:
Recognizes a protein of 75kDa, identified as gamma heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), u (IgM), Epsilon (IgE), or Delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Antigen: IgM
Dilution: Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml, SDS-Page
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Accession No.: P01871
Gene ID (Entrez): 3507
Immunogen: Recombinant heavy chain of human IgM
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Molecular Weight of Antigen: 75 kDa
Clone: IM260
Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Immunology
Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias: AGM1, DKFZp686I15196, DKFZp686I15212, FLJ00385, immunoglobulin heavy constant mu, MGC104996, MGC52291, MU, VH
Gene Symbols: IGHM
Isotype: IgG1 κ
Purification Method: Protein A purified
Test Specificity: Recognizes a protein of 75kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.
Antigen:
IgM
Dilution:
Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 0.5-1ug/ml, Immunoprecipitation 0.5-1ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/ml, SDS-Page
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Accession No.:
P01871
Gene ID (Entrez):
3507
Immunogen:
Recombinant heavy chain of human IgM
Primary or Secondary:
Primary
Content And Storage:
Store at 4C.
Molecular Weight of Antigen:
75 kDa
Clone:
IM260
Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Immunology
Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias:
AGM1, DKFZp686I15196, DKFZp686I15212, FLJ00385, immunoglobulin heavy constant mu, MGC104996, MGC52291, MU, VH
Gene Symbols:
IGHM
Isotype:
IgG1 κ
Purification Method:
Protein A purified
Test Specificity:
Recognizes a protein of 75kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This MAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.