7105591
Cytokeratin 8/18 Antibody (K8.8 + DC10), Novus Biologicals™
Mouse Monoclonal Antibody
Manufacturer: Fischer Scientific
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Antigen
Cytokeratin 8/18
Dilution
Western Blot 0.5-1.0ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/mlimmunohistochemistry-Paraffin 0.5-1ug/ml, SDS-Page
Classification
Monoclonal
Form
Purified
Regulatory Status
RUO
Target Species
Human
Gene Accession No.
P05787
Gene ID (Entrez)
3856
Immunogen
Keratin preparation from a human carcinoma (K8.8); PMC-42 human breast carcinoma cells (DC10)
Primary or Secondary
Primary
Content And Storage
Store at 4C.
Clone
K8.8 + DC10
Applications
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Conjugate
Unconjugated
Host Species
Mouse
Research Discipline
Cancer, Cytoskeleton Markers
Formulation
PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias
CARD2, CK8, CK-8, CYK8cytokeratin 8, Cytokeratin-8, K2C8, K8cytokeratin-8, keratin 8, keratin, type II cytoskeletal 8, keratin-8, KO, Type-II keratin Kb8
Gene Symbols
KRT8
Isotype
IgG
Purification Method
Protein A purified
Test Specificity
Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.Immunohistochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3.
Related Products
Description
- Cytokeratin 8/18 Monoclonal specifically detects Cytokeratin 8/18 in Human samples
- It is validated for Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Flow (Intracellular).
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Antigen: Cytokeratin 8/18
Dilution: Western Blot 0.5-1.0ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/mlimmunohistochemistry-Paraffin 0.5-1ug/ml, SDS-Page
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Accession No.: P05787
Gene ID (Entrez): 3856
Immunogen: Keratin preparation from a human carcinoma (K8.8); PMC-42 human breast carcinoma cells (DC10)
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Clone: K8.8 + DC10
Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cancer, Cytoskeleton Markers
Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias: CARD2, CK8, CK-8, CYK8cytokeratin 8, Cytokeratin-8, K2C8, K8cytokeratin-8, keratin 8, keratin, type II cytoskeletal 8, keratin-8, KO, Type-II keratin Kb8
Gene Symbols: KRT8
Isotype: IgG
Purification Method: Protein A purified
Test Specificity: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.Immunohistochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3.
Antigen:
Cytokeratin 8/18
Dilution:
Western Blot 0.5-1.0ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug protein lysate, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1ug/mlimmunohistochemistry-Paraffin 0.5-1ug/ml, SDS-Page
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Accession No.:
P05787
Gene ID (Entrez):
3856
Immunogen:
Keratin preparation from a human carcinoma (K8.8); PMC-42 human breast carcinoma cells (DC10)
Primary or Secondary:
Primary
Content And Storage:
Store at 4C.
Clone:
K8.8 + DC10
Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Cancer, Cytoskeleton Markers
Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias:
CARD2, CK8, CK-8, CYK8cytokeratin 8, Cytokeratin-8, K2C8, K8cytokeratin-8, keratin 8, keratin, type II cytoskeletal 8, keratin-8, KO, Type-II keratin Kb8
Gene Symbols:
KRT8
Isotype:
IgG
Purification Method:
Protein A purified
Test Specificity:
Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.Immunohistochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3.
Antigen: Chorionic Gonadotropin beta Chain (HCG beta)
Dilution: Western Blot 0.5-1ug/ml, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human
Gene Accession No.: P01233
Gene ID (Entrez): 1082
Immunogen: Recombinant hCG beta protein
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Clone: SPM105
Applications: Western Blot, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cancer
Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias: CGB3choriogonadotropin subunit beta, CGB5, CGB7, CGB8, CG-beta, Chorionic gonadotrophin chain beta, chorionic gonadotropin beta 3 subunit, chorionic gonadotropin beta chain, chorionic gonadotropin beta subunit, chorionic gonadotropin, beta polypeptide, hCGB
Gene Symbols: CGB
Isotype: IgG1 κ
Purification Method: Protein A purified
Test Specificity: This MAb reacts with a protein of 22kDa, identified as beta sub-unit of HCG. It does not cross react with the alpha sub-unit.
Antigen:
Chorionic Gonadotropin beta Chain (HCG beta)
Dilution:
Western Blot 0.5-1ug/ml, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human
Gene Accession No.:
P01233
Gene ID (Entrez):
1082
Immunogen:
Recombinant hCG beta protein
Primary or Secondary:
Primary
Content And Storage:
Store at 4C.
Clone:
SPM105
Applications:
Western Blot, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen)
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Cancer
Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias:
CGB3choriogonadotropin subunit beta, CGB5, CGB7, CGB8, CG-beta, Chorionic gonadotrophin chain beta, chorionic gonadotropin beta 3 subunit, chorionic gonadotropin beta chain, chorionic gonadotropin beta subunit, chorionic gonadotropin, beta polypeptide, hCGB
Gene Symbols:
CGB
Isotype:
IgG1 κ
Purification Method:
Protein A purified
Test Specificity:
This MAb reacts with a protein of 22kDa, identified as beta sub-unit of HCG. It does not cross react with the alpha sub-unit.
Antigen: GFAP
Dilution: Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Mouse, Rat, Porcine, Bovine, Chicken, Rabbit
Gene Accession No.: P14136
Gene ID (Entrez): 2670
Immunogen: GFAP isolated from pig spinal cord
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Clone: SPM248
Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Astrocyte Markers, Cancer, Cellular Markers, Cytoskeleton Markers, Neuronal Stem Cell Markers, Neuroscience, Stem Cell Markers
Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias: FLJ45472, GFAP astrocytes, glial fibrillary acidic protein
Gene Symbols: GFAP
Isotype: IgG1
Purification Method: Protein G purified
Test Specificity: This MAb recognizes a protein of ∼50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
Antigen:
GFAP
Dilution:
Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Mouse, Rat, Porcine, Bovine, Chicken, Rabbit
Gene Accession No.:
P14136
Gene ID (Entrez):
2670
Immunogen:
GFAP isolated from pig spinal cord
Primary or Secondary:
Primary
Content And Storage:
Store at 4C.
Clone:
SPM248
Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Astrocyte Markers, Cancer, Cellular Markers, Cytoskeleton Markers, Neuronal Stem Cell Markers, Neuroscience, Stem Cell Markers
Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias:
FLJ45472, GFAP astrocytes, glial fibrillary acidic protein
Gene Symbols:
GFAP
Isotype:
IgG1
Purification Method:
Protein G purified
Test Specificity:
This MAb recognizes a protein of ∼50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
Antigen: Cytokeratin 18
Dilution: Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml
Classification: Monoclonal
Form: Purified
Regulatory Status: RUO
Target Species: Human, Bovine (Negative), Canine (Negative), Hamster (Negative), Mouse (Negative), Porcine (Negative), Rat (Negative)
Gene Accession No.: P05783
Gene ID (Entrez): 3875
Immunogen: PMC-42 human breast carcinoma cells
Primary or Secondary: Primary
Content And Storage: Store at 4C.
Clone: SPM265
Applications: Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Conjugate: Unconjugated
Host Species: Mouse
Research Discipline: Cancer, Cell Biology, Cellular Markers, Cytoskeleton Markers
Formulation: PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias: Cell proliferation-inducing gene 46 protein, cell proliferation-inducing protein 46, CK-18, CYK18, cytokeratin 18, cytokeratin-18, K18, keratin 18, keratin, type I cytoskeletal 18, keratin-18
Gene Symbols: KRT18
Isotype: IgG1
Purification Method: Protein G purified
Test Specificity: This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
Antigen:
Cytokeratin 18
Dilution:
Western Blot 0.5-1ug/ml, Flow Cytometry 0.5-1ug/million cells, Immunocytochemistry/Immunofluorescence 1-2ug/ml, Immunoprecipitation 1-2ug/500ug, Immunohistochemistry-Paraffin 0.5-1ug/ml, Immunohistochemistry-Frozen 0.5-1.0ug/ml
Classification:
Monoclonal
Form:
Purified
Regulatory Status:
RUO
Target Species:
Human, Bovine (Negative), Canine (Negative), Hamster (Negative), Mouse (Negative), Porcine (Negative), Rat (Negative)
Gene Accession No.:
P05783
Gene ID (Entrez):
3875
Immunogen:
PMC-42 human breast carcinoma cells
Primary or Secondary:
Primary
Content And Storage:
Store at 4C.
Clone:
SPM265
Applications:
Western Blot, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin)
Conjugate:
Unconjugated
Host Species:
Mouse
Research Discipline:
Cancer, Cell Biology, Cellular Markers, Cytoskeleton Markers
Formulation:
PBS with 0.05% BSA. with 0.05% Sodium Azide
Gene Alias:
Cell proliferation-inducing gene 46 protein, cell proliferation-inducing protein 46, CK-18, CYK18, cytokeratin 18, cytokeratin-18, K18, keratin 18, keratin, type I cytoskeletal 18, keratin-18
Gene Symbols:
KRT18
Isotype:
IgG1
Purification Method:
Protein G purified
Test Specificity:
This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.